breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 18,802 C→A L30I (CTT→ATT)  nhaR → DNA‑binding transcriptional activator NhaR
RA 73,943 C→A R193R (CGG→CGT thiP ← thiamine ABC transporter membrane subunit
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 457,461 G→T L12F (TTG→TTT clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,477,061 C→A R640S (CGT→AGT)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,610,150 G→C G177G (GGC→GGG uxaB ← tagaturonate reductase
JC JC 1,652,297 IS2 (–) +5 bp pseudogene (786‑790/1197 nt) intQ → Qin prophage; putative defective integrase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,230,190 G→T R66S (CGC→AGC)  dusC ← tRNA‑dihydrouridine(16) synthase
RA 2,299,100 A→T I160N (ATC→AAC)  napH ← ferredoxin‑type protein NapH
RA 3,145,897 G→T P122Q (CCA→CAA)  hybO ← hydrogenase 2 small subunit
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,370,163 G→T S63* (TCG→TAG)  nanK ← N‑acetylmannosamine kinase
RA 3,414,536 C→A L225M (CTG→ATG)  acrE → multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,542,507 C→A L701M (CTG→ATG)  feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,966,245 G→T Q5H (CAG→CAT rhoL → rho operon leader peptide
RA 4,085,782 C→A G14V (GGC→GTC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,248,690 C→A F240L (TTC→TTA lamB → maltose outer membrane channel/phage lambda receptor protein
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102985 3104055 1071 22 [21] [21] 22 [mutY] [mutY]
* * ÷ NC_000913 3423768–3424234 3424538–3424238 5–771 22 [20] [21] 22 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =61 (0.740)29 (0.400) 25/246 1.5 29.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 88 (1.200)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780561 (0.740)18 (0.250) 15/246 2.9 20.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 88 (1.200)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)110 (1.370) 68/270 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =152 (1.840)131 (1.600) 59/276 0.1 63.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)107 (1.290) 61/278 0.1 93.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA