breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 268,468 C→A G165* (GGA→TGA)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein YkfA
RA 425,745 G→T A245A (GCG→GCT queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,837,896 C→A Q229K (CAG→AAG)  ynjC → putative ABC transporter membrane subunit YnjC
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,290,897 G→T R133L (CGC→CTC)  narP → DNA‑binding transcriptional dual regulator NarP
RA 2,465,546 C→A V82V (GTC→GTA yfdC → inner membrane protein YfdC
RA 2,972,744 C→A V373V (GTG→GTT lplT ← lysophospholipid transporter
RA 2,984,980 G→T Q205K (CAA→AAA)  yqeF ← putative acyltransferase
RA 3,075,256 G→T G309G (GGC→GGA yggF ← fructose 1,6‑bisphosphatase YggF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,356,973 G→T S750I (AGC→ATC)  gltB → glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,919,690 C→A P56P (CCG→CCT atpA ← ATP synthase F1 complex subunit alpha
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,328,651 T→C F506S (TTT→TCT)  crfC → clamp‑binding sister replication fork colocalization protein
RA 4,438,942 C→A P107P (CCG→CCT ytfJ ← protein YtfJ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104070 1101 48 [46] [46] 47 mutY mutY
* * ÷ NC_000913 3423760–3424234 3424540–3424239 6–781 48 [46] [45] 47 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =210 (1.140)66 (0.400) 40/244 1.8 28.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 152 (0.930)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)339 (1.890) 121/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =368 (1.990)337 (1.840) 105/274 0.1 64.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)257 (1.390) 101/276 0.1 96.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA