breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 230,984 C→A intergenic (‑103/‑138) yafC ← / → yafD putative LysR family transcriptional regulator YafC/endonuclease/exonuclease/phosphatase domain‑containing protein YafD
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 757,112 C→A G402G (GGC→GGA sdhA → succinate:quinone oxidoreductase, FAD binding protein
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 947,146 T→C intergenic (+127/‑83) ycaD → / → ycaM putative transporter YcaD/putative transporter YcaM
RA 990,178 G→T A60E (GCG→GAG)  pncB ← nicotinate phosphoribosyltransferase
RA 1,572,262 T→C intergenic (‑217/+145) gadB ← / ← pqqL glutamate decarboxylase B/putative zinc peptidase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,789,666 G→T K18N (AAG→AAT ydiE → PF10636 family protein YdiE
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,039,387 G→T intergenic (+18/‑91) yedX → / → msrP hydroxyisourate hydrolase/transthyretin‑related protein/periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,198,532 G→T intergenic (+1234/‑1747) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,282,209 G→T P526P (CCG→CCT radD → putative DNA repair helicase RadD
RA 2,291,850 C→A L187F (TTG→TTT ccmH ← holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
RA 3,007,402 G→T G381C (GGT→TGT)  ygeW → putative carbamoyltransferase YgeW
RA 3,238,222 C→T R304R (CGC→CGT ygjR → putative oxidoreductase YgjR
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,771,593 C→A S51I (AGC→ATC)  yibI ← DUF3302 domain‑containing protein YibI
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,868,172 C→A E380* (GAA→TAA)  yidR ← DUF3748 domain‑containing galacturonate catabolism protein YidR
RA 4,227,781 G→T G17G (GGG→GGT yjbB → putative inorganic phosphate export protein
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,304,229 G→T R59R (CGA→AGA)  ytcA ← putative lipoprotein YtcA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1287146–1287147 1287457 311–312 64 [63] [63] 69 [rttR]–tyrV [rttR],[tpr],tyrV
* * ÷ NC_000913 3102973 3104054 1082 64 [61] [61] 64 [mutY] [mutY]
* * ÷ NC_000913 3423752–3424234 3424546–3424238 5–795 66 [63] [63] 65 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =46 (0.210)327 (1.700) 109/244 0.0 88.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 43 (0.220)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780546 (0.210)167 (0.870) 76/244 0.4 80.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 43 (0.220)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)339 (1.600) 116/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =359 (1.650)418 (1.930) 126/274 0.0 69.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 6 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)235 (1.080) 101/276 0.2 95.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 11 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA