breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 308,283 C→A K350N (AAG→AAT ecpC ← putative fimbrial usher protein EcpC
RA 754,798 T→C intergenic (‑17/‑379) ybgU ← / → sdhC protein YbgU/succinate:quinone oxidoreductase, membrane protein SdhC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,048,835 C→A Q166H (CAG→CAT gfcB ← lipoprotein GfcB
RA 1,066,333 G→A W250* (TGG→TAG)  agp → glucose‑1‑phosphatase
JC JC 1,412,941 +G :: IS5 (–) +3 bp coding (293‑295/1236 nt) intR ← Rac prophage; putative integrase
RA 1,544,405 G→T pseudogene (315/336 nt) yddJ ← uncharacterized protein YddJ
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,839,353 G→T intergenic (‑89/‑171) ascG ← / → ascF DNA‑binding transcriptional repressor AscG/beta‑glucoside specific PTS enzyme IIBC component
RA 3,123,635 G→T R57R (CGC→CGA glcB ← malate synthase G
RA 3,327,503 C→A T54T (ACG→ACT rlmE ← 23S rRNA 2'‑O‑ribose U2552 methyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,781,948 G→T G205G (GGC→GGA cysE ← serine acetyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,388,984 C→A E129D (GAG→GAT mscM ← miniconductance mechanosensitive channel MscM

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102973 3104077 1105 28 [25] [27] 28 mutY mutY
* * ÷ NC_000913 3423768–3424233 3424530–3424239 7–763 29 [27] [27] 29 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =73 (0.670)129 (1.330) 76/244 0.0 64.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 75 (0.770)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780573 (0.670)59 (0.610) 39/244 1.0 45.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 75 (0.770)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)145 (1.360) 75/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =160 (1.460)176 (1.620) 94/274 0.0 68.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)122 (1.110) 73/276 0.1 96.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA