breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 365,942 C→A G122C (GGT→TGT)  lacZ ← beta‑galactosidase
RA 498,722 G→T G223V (GGG→GTG)  hemH → ferrochelatase
RA 597,568 G→T P30P (CCG→CCT cusB → copper/silver export system membrane fusion protein
RA 873,703 A→G E242G (GAA→GGA)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,185,878 T→A V12E (GTG→GAG)  pepT → peptidase T
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,039,591 G→T L38F (TTG→TTT msrP → periplasmic protein‑L‑methionine sulfoxide reductase catalytic subunit
RA 2,117,717 C→A V230F (GTT→TTT)  wcaK ← putative colanic acid biosynthesis pyruvyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,365,994 G→T V29F (GTT→TTT)  arnB → UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
RA 2,747,354 G→T L148I (CTT→ATT)  ffh ← signal recognition particle protein component
RA 3,057,232 C→A A393A (GCG→GCT serA ← phosphoglycerate dehydrogenase
RA 3,127,980 G→T P14P (CCC→CCA glcD ← glycolate dehydrogenase, putative FAD‑linked subunit
RA 3,303,260 G→T D258Y (GAC→TAC)  yhbV → putative peptidase YhbV
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
JC JC 3,465,750 IS2 (+) +5 bp coding (208‑212/678 nt) gspO → Type II secretion system prepilin peptidase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,391,069 G→T A147A (GCC→GCA rsgA ← ribosome small subunit‑dependent GTPase A
RA 4,458,057 G→T S33S (TCG→TCT pmbA → metalloprotease PmbA
RA 4,458,689 C→A A244E (GCG→GAG)  pmbA → metalloprotease PmbA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102986 3104083 1098 13 [12] [12] 13 mutY mutY
* * ÷ NC_000913 3423780–3424233 3424545–3424242 10–766 13 [12] [11] 13 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)9 (0.220) 8/190 2.5 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1207790 =72 (1.200)19 (0.360) 16/244 1.7 27.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 36 (0.680)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)81 (1.390) 51/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =110 (1.840)71 (1.190) 46/274 0.2 55.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.080)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)80 (1.340) 52/276 0.1 96.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA