Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,274	G→T	S353R (AGC→AGA)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,274	0	G	T	100.0%	318.3 / NA	92	S353R (AGC→AGA)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/45);  total (47/45)

CCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGGCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTC  >  NC_000913/308140‑308406                                                                                                                                       |                                                                                                                                     ccGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTaaa                                                                                                                                 >  2:595406/1‑140 (MQ=255)     aGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGc                                                                                                                             >  1:52480/1‑140 (MQ=255)     aGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGc                                                                                                                             >  2:477727/1‑140 (MQ=255)           aGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTg                                                                                                                       >  1:898955/1‑140 (MQ=255)               aCCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGAccc                                                                                                                   >  2:1314132/1‑140 (MQ=255)                 cTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCAACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGc                                                                                                                 >  2:498584/1‑140 (MQ=255)                     tAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTggg                                                                                                             >  1:645746/1‑140 (MQ=255)                       gCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTg                                                                                                           >  1:187026/1‑140 (MQ=255)                       gCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTg                                                                                                           >  1:1636681/1‑140 (MQ=255)                           aCTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGAccc                                                                                                                   >  1:975080/1‑128 (MQ=255)                           aCTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGAccc                                                                                                                   <  2:975080/128‑1 (MQ=255)                           aCTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCg                                                                                                                  <  1:1051081/129‑1 (MQ=255)                           aCTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCg                                                                                                                  >  2:1051081/1‑129 (MQ=255)                              ctctTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGc                                                                                                    >  1:1541779/1‑140 (MQ=255)                              ctctTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGc                                                                                                    >  1:1011538/1‑140 (MQ=255)                                 tttAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGa                                                                                                 >  2:702844/1‑140 (MQ=255)                                 tttAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGa                                                                                                 >  2:1931581/1‑140 (MQ=255)                                      cTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcg                                                                                            <  2:1753735/140‑1 (MQ=255)                                      cTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcg                                                                                            <  2:898955/140‑1 (MQ=255)                                      cTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcg                                                                                            <  1:1622894/140‑1 (MQ=255)                                       tGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgc                                                                                           >  1:1468190/1‑140 (MQ=255)                                        ggTGGCGTCTTTTTCCCGTTTACGAACCAGGGTTCCATATGAAGGTTACCGCCCCATCCCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgcg                                                                                          <  2:978071/140‑1 (MQ=255)                                        ggTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATCCCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgcg                                                                                          <  2:1630296/140‑1 (MQ=255)                                        ggTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgcg                                                                                          >  2:1065945/1‑140 (MQ=255)                                        ggTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgcg                                                                                          <  2:799474/140‑1 (MQ=255)                                        gctCGCGTTTTTTTCCCGTTTTCCGCCAAGCGACCCATATAAAAGCTCCCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCAcgcg                                                                                          <  2:489022/138‑1 (MQ=255)                                         gttgcgTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGa                                                                                         >  1:303809/4‑140 (MQ=255)                                         gTCGCGTCTTTTCCCCGTTTCCCGCCCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGa                                                                                         <  2:1616810/140‑1 (MQ=255)                                                    ttCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGAt                                                                              <  2:1554440/140‑1 (MQ=255)                                                     tCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATc                                                                             <  1:481687/140‑1 (MQ=255)                                                      cccGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCa                                                                            >  1:1110466/1‑140 (MQ=255)                                                        cGTTTCCCGCCCAGCGATCCATATGAAAGCTCCCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCAcc                                                                          <  2:523509/140‑1 (MQ=255)                                                          ttttCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTc                                                                        >  2:1441467/1‑140 (MQ=255)                                                          ttttCCAACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTc                                                                        <  1:498584/140‑1 (MQ=255)                                                                  ccAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCca                                                                <  2:1541779/140‑1 (MQ=255)                                                                   cAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCcac                                                               <  1:330969/140‑1 (MQ=255)                                                                   cAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCcac                                                               <  1:595406/140‑1 (MQ=255)                                                                   cAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCcac                                                               <  2:645746/140‑1 (MQ=255)                                                                     gCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACAt                                                             >  1:149886/1‑140 (MQ=255)                                                                      cGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATc                                                            >  1:1561104/1‑140 (MQ=255)                                                                           cATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTaaa                                                       <  1:1931581/140‑1 (MQ=255)                                                                           cATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTaaa                                                       <  1:702844/140‑1 (MQ=255)                                                                             aataaaaGTTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATc                                                     <  2:1468190/136‑1 (MQ=255)                                                                                gAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCg                                                  <  1:414011/140‑1 (MQ=255)                                                                                 aaaGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGt                                                 <  1:728309/140‑1 (MQ=255)                                                                                   aGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTAc                                               >  1:1076363/1‑140 (MQ=255)                                                                                   aGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTAc                                               >  1:689689/1‑140 (MQ=255)                                                                                   aGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTAc                                               >  2:503760/1‑140 (MQ=255)                                                                                   aGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTAc                                               >  2:1349485/1‑140 (MQ=255)                                                                                     cTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATc                                                     <  1:1485244/132‑1 (MQ=255)                                                                                     cTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATc                                                     >  2:1485244/1‑132 (MQ=255)                                                                                     cTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACgg                                             <  1:1349485/140‑1 (MQ=255)                                                                                     cTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACgg                                             >  2:337503/1‑140 (MQ=255)                                                                                     cTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACgg                                             <  1:503760/140‑1 (MQ=255)                                                                                        ccGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAg                                          >  1:538389/1‑140 (MQ=255)                                                                                            cccATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGAccc                                      <  2:149886/140‑1 (MQ=255)                                                                                                tACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCggg                                  >  1:1213042/1‑140 (MQ=255)                                                                                                  ccTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTa                                <  2:1076363/140‑1 (MQ=255)                                                                                                  ccTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTa                                <  1:1669009/140‑1 (MQ=255)                                                                                                   cTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTAt                               <  2:852259/140‑1 (MQ=255)                                                                                                   cTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTAt                               <  1:1491449/140‑1 (MQ=255)                                                                                                    tGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  2:689689/140‑1 (MQ=255)                                                                                                    tGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  1:1314132/140‑1 (MQ=255)                                                                                                    tGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  2:1110466/140‑1 (MQ=255)                                                                                                    cgCCACGCCAGTTGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCACCTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTaac                              <  2:169386/139‑3 (MQ=255)                                                                                                     gCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTa                                >  1:568125/1‑137 (MQ=255)                                                                                                     gCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTa                                <  2:568125/137‑1 (MQ=255)                                                                                                     gCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              >  1:118804/1‑139 (MQ=255)                                                                                                     gCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  2:118804/139‑1 (MQ=255)                                                                                                      ccACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              >  1:1889986/1‑138 (MQ=255)                                                                                                      ccACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  2:1889986/138‑1 (MQ=255)                                                                                                      ccACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCa                            >  1:1471870/1‑140 (MQ=255)                                                                                                      ccACGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCa                            >  1:1399523/1‑140 (MQ=255)                                                                                                        aCGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACt                          >  1:1833909/1‑140 (MQ=255)                                                                                                        aCGCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACt                          >  1:1461367/1‑140 (MQ=255)                                                                                                          gCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGAccc                                      >  2:715163/1‑126 (MQ=255)                                                                                                          gCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGAccc                                      <  1:715163/126‑1 (MQ=255)                                                                                                          gCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTc                        >  2:970535/1‑140 (MQ=255)                                                                                                          gCCAGTGGTGCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTc                        >  1:537256/1‑140 (MQ=255)                                                                                                              gtggtgCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGa                    <  2:1213042/140‑1 (MQ=255)                                                                                                               tggtgCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGAt                   >  2:1924132/1‑140 (MQ=255)                                                                                                               tggtgCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGAt                   >  2:1150733/1‑140 (MQ=255)                                                                                                                  tgCACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGc                <  2:52480/140‑1 (MQ=255)                                                                                                                    cACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              >  2:987786/1‑124 (MQ=255)                                                                                                                    cACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATc                              <  1:987786/124‑1 (MQ=255)                                                                                                                    cACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGccc              >  2:1919238/1‑140 (MQ=255)                                                                                                                    cACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGccc              >  2:1212787/1‑140 (MQ=255)                                                                                                                    cACCGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGccc              >  1:171170/1‑140 (MQ=255)                                                                                                                       cGACGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATa           <  2:1011538/140‑1 (MQ=255)                                                                                                                         aCGCCGCGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGt         >  1:217672/1‑140 (MQ=255)                                                                                                                          cgccgcGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTg        <  1:477727/140‑1 (MQ=255)                                                                                                                            ccgcGCCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGaa      <  2:1555519/140‑1 (MQ=255)                                                                                                                                gcCCCCGTCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTc  <  2:1461367/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CCGGAGGTCGAGGCACCTGCTAGCCAACTCTCTTTAGCTGGTCGCGTCTTTTTCCCGTTTTCCGACCAGCGATCCATATGAAAGCTACCGCCCCATACCTGCCACGCCAGTGGTGCACCGACGCCGCGCCCCCGGCTAAACAGCTTATTGACCCGCTGGGTGCGTTTGCTGATCACGCGACCGTTAACGATCACCTCAACTTCCACATCGTAAATCCCGTACGGTAGACCCCGGGTATCCACTTCATGATTGCCCATAGTGAAGTTC  >  NC_000913/308140‑308406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑