Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,386	C→A	L403F (TTG→TTT)	nagC ←	DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,386	0	C	A	100.0%	324.0 / NA	91	L403F (TTG→TTT)	nagC	DNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/39);  total (52/39)

CCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATG  >  NC_000913/700251‑700521                                                                                                                                        |                                                                                                                                        ccGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAAt                                                                                                                                     >  1:161970/1‑140 (MQ=255) ccGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAAt                                                                                                                                     >  1:388264/1‑140 (MQ=255)   gCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGc                                                                                                                                   <  1:1469393/140‑1 (MQ=255)    cTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGc                                                                                                                                   >  1:1445042/1‑139 (MQ=255)    cTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGc                                                                                                                                   <  2:1445042/139‑1 (MQ=255)    cTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCt                                                                                                                                  >  2:1701316/1‑140 (MQ=255)     tGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTg                                                                                                                                 <  2:700541/140‑1 (MQ=255)               aCGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATa                                                                                                                       <  2:1683751/140‑1 (MQ=255)                   cGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGt                                                                                                                   >  2:1184986/1‑140 (MQ=255)                    gACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGtt                                                                                                                  >  2:488087/1‑140 (MQ=255)                      cGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACCATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGa                                                                                                                >  1:45294/1‑140 (MQ=255)                       gTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAg                                                                                                               <  2:396140/140‑1 (MQ=255)                        ttATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGc                                                                                                              >  1:1335551/1‑140 (MQ=255)                            cGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATgg                                                                                                          <  1:187917/140‑1 (MQ=255)                                 aTCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGt                                                                                                     >  1:1808122/1‑140 (MQ=255)                                      cACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTAc                                                                                                <  1:298069/140‑1 (MQ=255)                                        cGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCGAAATACCGTTGAGCATgg                                                                                                          >  2:1758104/1‑128 (MQ=255)                                        cGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCGAAATACCGTTGAGCATgg                                                                                                          <  1:1758104/128‑1 (MQ=255)                                           cGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAgcg                                                                                           >  2:1131270/1‑140 (MQ=255)                                                      cAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCg                                                                                >  1:1764792/1‑140 (MQ=255)                                                           tACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGc                                                                           >  2:908936/1‑140 (MQ=255)                                                            aCATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGcc                                                                          <  2:1124235/140‑1 (MQ=255)                                                             cATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCg                                                                         <  1:215702/140‑1 (MQ=255)                                                             cATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCg                                                                         <  1:1917177/140‑1 (MQ=255)                                                                ttttAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGc                                                                      <  1:488087/140‑1 (MQ=255)                                                                    aaTGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTg                                                                  >  1:1644679/1‑140 (MQ=255)                                                                       ggTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                               <  1:1648608/140‑1 (MQ=255)                                                                       ggTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATc                                                               <  2:921398/140‑1 (MQ=255)                                                                        gTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATcc                                                              >  2:1722427/1‑140 (MQ=255)                                                                         tCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCa                                                             <  2:463382/140‑1 (MQ=255)                                                                          cATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAg                                                            >  2:523653/1‑140 (MQ=255)                                                                                  aCCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAAc                                                    >  2:924468/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  2:1763950/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:744358/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:940913/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:1100150/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:1317054/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:143023/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  1:1733151/1‑140 (MQ=255)                                                                                   cccAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACg                                                   >  2:688029/1‑140 (MQ=255)                                                                                     cAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTa                                                 >  1:1558189/1‑140 (MQ=255)                                                                                        aaTATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTacca                                              >  1:1296041/1‑140 (MQ=255)                                                                                        aaTATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTacca                                              >  1:571/1‑140 (MQ=255)                                                                                         atatTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTaccac                                             <  2:1364219/140‑1 (MQ=255)                                                                                             tcacaacaATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGATGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                         >  2:1447096/3‑140 (MQ=255)                                                                                             tGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCt                                                          >  1:1404723/1‑123 (MQ=255)                                                                                             tGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCt                                                          <  2:1404723/123‑1 (MQ=255)                                                                                             tGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                         >  1:629899/1‑140 (MQ=255)                                                                                             tGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGc                                         >  2:255553/1‑140 (MQ=255)                                                                                              gACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCa                                        >  1:761493/1‑140 (MQ=255)                                                                                              gACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCa                                        >  1:343067/1‑140 (MQ=255)                                                                                                 aacaaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAt                                     <  2:571/140‑1 (MQ=255)                                                                                                  acaaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAtt                                    >  1:225528/1‑140 (MQ=255)                                                                                                  acaaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGAtt                                    >  2:898304/1‑140 (MQ=255)                                                                                                    aaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAg                                  >  1:872995/1‑140 (MQ=255)                                                                                                    aaTAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAg                                  >  1:1334266/1‑140 (MQ=255)                                                                                                      tAAGCGCCACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTg                                >  1:1912365/1‑140 (MQ=255)                                                                                                             cACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACg                         >  1:1908671/1‑140 (MQ=255)                                                                                                             cACTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACg                         <  1:688029/140‑1 (MQ=255)                                                                                                              aCTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGc                        >  2:558075/1‑140 (MQ=255)                                                                                                              aCTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGc                        >  1:1174651/1‑140 (MQ=255)                                                                                                              aCTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGc                        >  2:2283/1‑140 (MQ=255)                                                                                                              aCTATAAAAGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGc                        >  2:1079381/1‑140 (MQ=255)                                                                                                                   aaaaGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCa                   <  2:744358/140‑1 (MQ=255)                                                                                                                    aaaGCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAg                  <  2:872995/140‑1 (MQ=255)                                                                                                                       gCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAgcgc               <  1:924468/140‑1 (MQ=255)                                                                                                                       gCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAgcgc               <  2:1803094/140‑1 (MQ=255)                                                                                                                       gCACATTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAgcgc               <  2:143023/140‑1 (MQ=255)                                                                                                                        cacaTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGcc              <  2:1362566/140‑1 (MQ=255)                                                                                                                        cacaTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGcc              <  2:985220/140‑1 (MQ=255)                                                                                                                         acaTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCt             <  2:1808122/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  2:241993/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  1:130061/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  1:1269252/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  1:1503482/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  1:1701316/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  2:982854/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  1:523653/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  2:1334266/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  1:678609/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  1:718742/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  2:1699454/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            >  2:101430/1‑140 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  2:1296041/140‑1 (MQ=255)                                                                                                                          caTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTg            <  2:1317054/140‑1 (MQ=255)                                                                                                                           aTTAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTgg           >  1:1530529/1‑140 (MQ=255)                                                                                                                            ttAATTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTggg          >  1:1882977/1‑140 (MQ=255)                                                                                                                               aTTTTCCAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTAt       <  1:101430/140‑1 (MQ=255)                                                                                                                                    ccAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg  <  1:558075/140‑1 (MQ=255)                                                                                                                                    ccAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg  <  2:1530529/140‑1 (MQ=255)                                                                                                                                    ccAGAAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATg  <  2:1335551/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCACGCCGTCGATATCGCAAATTACATTTTTAATGGTCATGGACTACCCAGAATATTGACAACAATAAGCGCCACTATAAAAGCACATTAATTTTCCAGCAAATGCTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATG  >  NC_000913/700251‑700521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑