Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,496,597	C→A	intergenic (+32/‑324)	lpxP → / → ypdK	palmitoleoyl acyltransferase/putative membrane protein YpdK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,496,597	0	C	A	100.0%	241.7 / NA	68	intergenic (+32/‑324)	lpxP/ypdK	palmitoleoyl acyltransferase/putative membrane protein YpdK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/32);  total (36/32)

AACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGTAAATAAGAAAA  >  NC_000913/2496461‑2496733                                                                                                                                         |                                                                                                                                         aaCAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGc                                                                                                                                       <  1:896301/140‑1 (MQ=255)   cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAgg                                                                                                                                        >  1:20438/1‑137 (MQ=255)   cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCa                                                                                                                                     >  2:871528/1‑140 (MQ=255)   cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCa                                                                                                                                     >  1:684713/1‑140 (MQ=255)   cAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCa                                                                                                                                     >  2:1405735/1‑140 (MQ=255)           tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAAcc                                                                                                                             >  2:515840/1‑140 (MQ=255)           tCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAAcc                                                                                                                             >  1:66238/1‑140 (MQ=255)                aaaGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATg                                                                                                                        >  2:1184954/1‑140 (MQ=255)                           gcgcgcACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATg                                                                                                                        >  1:145213/1‑129 (MQ=255)                           gcgcgcACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATg                                                                                                                        <  2:145213/129‑1 (MQ=255)                              cgcACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCttt                                                                                                          >  2:1332032/1‑140 (MQ=255)                              cgcACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCttt                                                                                                          >  1:881754/1‑140 (MQ=255)                              cgcACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCttt                                                                                                          >  1:974517/1‑140 (MQ=255)                                cACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCttttt                                                                                                        >  2:331048/1‑140 (MQ=255)                                    ggAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCtt                                                                                                    <  1:331048/140‑1 (MQ=255)                                    ggAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCtt                                                                                                    <  1:482425/140‑1 (MQ=255)                                    ggAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCtt                                                                                                    <  1:1332032/140‑1 (MQ=255)                                     gAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTa                                                                                                   <  2:1052336/140‑1 (MQ=255)                                           tACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGttt                                                                                             >  2:1172530/1‑140 (MQ=255)                                             ccTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGttttt                                                                                           >  1:578227/1‑140 (MQ=255)                                             ccTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGttttt                                                                                           >  2:723000/1‑140 (MQ=255)                                                ctGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTcc                                                                                        >  1:471564/1‑140 (MQ=255)                                                  ggATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCtt                                                                                      >  2:471400/1‑140 (MQ=255)                                                    aTCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCg                                                                                    <  2:1166708/140‑1 (MQ=255)                                                     tCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGc                                                                                   >  2:305293/1‑140 (MQ=255)                                                      ccACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCt                                                                                  <  2:974517/140‑1 (MQ=255)                                                      ccACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCt                                                                                  <  2:578227/140‑1 (MQ=255)                                                      ccACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCt                                                                                  <  2:881754/140‑1 (MQ=255)                                                        aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                                                                                <  1:871528/140‑1 (MQ=255)                                                        aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                                                                                >  2:47975/1‑140 (MQ=255)                                                        aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                                                                                <  1:1405735/140‑1 (MQ=255)                                                        aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                                                                                >  1:853629/1‑140 (MQ=255)                                                        aCCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGa                                                                                >  1:169039/1‑140 (MQ=255)                                                          cGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAat                                                                              <  1:1194156/140‑1 (MQ=255)                                                          cGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAat                                                                              <  1:515840/140‑1 (MQ=255)                                                           gTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAata                                                                             >  1:1057055/1‑140 (MQ=255)                                                            tCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAatat                                                                            <  2:684713/140‑1 (MQ=255)                                                                      aCGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAAttatta                                                                  <  1:1172530/140‑1 (MQ=255)                                                                          gcCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGt                                                              >  1:388855/1‑140 (MQ=255)                                                                           cccGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTc                                                             <  1:928218/140‑1 (MQ=255)                                                                           cccGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTc                                                             <  1:305293/140‑1 (MQ=255)                                                                                    agaATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTaa                                                    >  2:947521/1‑140 (MQ=255)                                                                                      aaTCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAAc                                                  >  2:639774/1‑140 (MQ=255)                                                                                        tcgtcgTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTc                                                             >  2:1344163/1‑127 (MQ=255)                                                                                        tcgtcgTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTc                                                             <  1:1344163/127‑1 (MQ=255)                                                                                        tcgtcgTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTg                                                >  2:1257486/1‑140 (MQ=255)                                                                                        tcgtcgTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTg                                                >  2:1140286/1‑140 (MQ=255)                                                                                            cgtTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGc                                            <  1:1184954/140‑1 (MQ=255)                                                                                            cgtTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGc                                            <  2:388855/140‑1 (MQ=255)                                                                                             gtTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGcc                                           <  2:66238/140‑1 (MQ=255)                                                                                                  aCATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGc                                            >  1:951764/1‑134 (MQ=255)                                                                                                  aCATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGc                                            <  2:951764/134‑1 (MQ=255)                                                                                                     tttAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTCTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATgag                                   <  2:1152899/140‑1 (MQ=255)                                                                                                      ttAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATgaga                                  <  1:1257486/140‑1 (MQ=255)                                                                                                      ttAACAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATgaga                                  <  2:1057055/140‑1 (MQ=255)                                                                                                        aaCAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATgaga                                  <  1:904735/138‑1 (MQ=255)                                                                                                        aaCAAACTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATgaga                                  >  2:904735/1‑138 (MQ=255)                                                                                                             aCTGCTCTTACTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGt                           >  2:554083/1‑140 (MQ=255)                                                                                                                      aCTCTTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTa                  >  1:273859/1‑140 (MQ=255)                                                                                                                       ctctTTCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTaa                 <  1:1140286/140‑1 (MQ=255)                                                                                                                           ttCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGt                           <  2:875477/126‑1 (MQ=255)                                                                                                                           ttCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGt                           >  1:875477/1‑126 (MQ=255)                                                                                                                           ttCCCTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGt             <  1:471400/140‑1 (MQ=255)                                                                                                                             cccTCCTCAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGTaa           >  2:260483/1‑140 (MQ=255)                                                                                                                                  ctcAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGTAAATAAg      >  2:13412/1‑140 (MQ=255)                                                                                                                                    cAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTaaaa               >  1:1372520/1‑129 (MQ=255)                                                                                                                                    cAAATAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTaaaa               <  2:1372520/129‑1 (MQ=255)                                                                                                                                      aaTAGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGTAAATAAGaaaa  <  1:639774/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AACAAGATTATCGAGAAAGAGATCATGCGCGCACCGGAGCAGTACCTCTGGATCCACCGTCGCTTTAAAACGCGCCCGGTGGGAGAATCGTCGTTGTACATTTAACAAACTGCTCTTACTCTTTCCCTCCTCAAATCGGCCATAATAACCTCATGGTTTTTATGGCTTTTTTCTTAAGGTTTTTTCCTTCGCTGAATATCTAATTATTACCGTCAGAAATTAAACTGTCGCCGATATGAGACACTTGTAAGTTACTAAAAGTAAATAAGAAAA  >  NC_000913/2496461‑2496733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑