Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,942,516	C→A	A18S (GCA→TCA)	gcvA ←	DNA‑binding transcriptional dual regulator GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,942,516	0	C	A	100.0%	249.6 / NA	71	A18S (GCA→TCA)	gcvA	DNA‑binding transcriptional dual regulator GcvA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/43);  total (28/43)

GTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCA  >  NC_000913/2942380‑2942646                                                                                                                                         |                                                                                                                                   gTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGtgatgc                                                                                                                                 >  1:996852/1‑140 (MQ=255) gTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGACTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGtgatgc                                                                                                                                 <  2:820611/140‑1 (MQ=255)   cagcagGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCgg                                                                                                                               <  1:558654/140‑1 (MQ=255)      cagGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCAt                                                                                                                            >  1:564223/1‑140 (MQ=255)      cagGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCAt                                                                                                                            <  1:1164876/140‑1 (MQ=255)           gTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCaaaa                                                                                                                       <  1:23447/140‑1 (MQ=255)            tGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCaaaaa                                                                                                                      >  1:824625/1‑140 (MQ=255)            tGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCaaaaa                                                                                                                      >  2:1295370/1‑140 (MQ=255)              aaCGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACt                                                                                                                    >  1:542389/1‑140 (MQ=255)              aaCGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACt                                                                                                                    >  2:345052/1‑140 (MQ=255)              aaCGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACt                                                                                                                    >  2:1230025/1‑140 (MQ=255)                  aTTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTa                                                                                                                <  2:1328192/140‑1 (MQ=255)                  aTTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTa                                                                                                                <  1:770918/140‑1 (MQ=255)                  aTTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTa                                                                                                                <  2:1210752/140‑1 (MQ=255)                  aTTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTa                                                                                                                <  1:345052/140‑1 (MQ=255)                        gcggcggAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCAt                                                                                                          >  1:1131909/1‑140 (MQ=255)                           gcggAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTTATTTGATGACTTACTACGGCTTGGGTCACAAAAAGCGCTTCTGCTGCGCGAGTGCAACTTAAATGGCGGGATGCGGCATCAAAAA‑TCTTTATGCATTTat                                                                                                      >  2:1401908/1‑139 (MQ=255)                             ggAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGc                                                                                                     <  2:564223/140‑1 (MQ=255)                                   gTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCggtggt                                                                                               >  1:227162/1‑140 (MQ=255)                                    ttttAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTa                                                                                              <  2:928144/140‑1 (MQ=255)                                    ttttAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTa                                                                                              <  2:597101/140‑1 (MQ=255)                                    ttttAGCCCCAAAAAATCCTCAAGAGACTTGATTTGAAGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTa                                                                                              <  2:368136/140‑1 (MQ=255)                                         gCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGc                                                                                                     >  1:842809/1‑128 (MQ=255)                                         gCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGc                                                                                                     <  2:842809/128‑1 (MQ=255)                                         gCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGt                                                                                         >  1:1015381/1‑140 (MQ=255)                                         gCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGt                                                                                         >  1:1284254/1‑140 (MQ=255)                                            ccAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTaa                                                                                             >  2:1293632/1‑133 (MQ=255)                                            ccAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTaa                                                                                             <  1:1293632/133‑1 (MQ=255)                                              aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGa                                                                                    >  2:1155802/1‑140 (MQ=255)                                              aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGa                                                                                    >  2:478286/1‑140 (MQ=255)                                               aaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGAc                                                                                   <  2:792597/140‑1 (MQ=255)                                                 aaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAATCTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACAt                                                                                 <  2:190641/140‑1 (MQ=255)                                                 aaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACAt                                                                                 <  2:1131909/140‑1 (MQ=255)                                                 aaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACAt                                                                                 <  1:96723/140‑1 (MQ=255)                                                        cAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAtt                                                                          <  2:542389/140‑1 (MQ=255)                                                               cTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACttt                                                                   <  2:1284254/140‑1 (MQ=255)                                                               cTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACttt                                                                   <  2:996852/140‑1 (MQ=255)                                                                  gatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGAt                                                                <  2:227162/140‑1 (MQ=255)                                                                   atttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATg                                                               >  1:61682/1‑140 (MQ=255)                                                                        atgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTaaa                                                          >  2:1304660/1‑140 (MQ=255)                                                                         tgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAAt                                                         <  1:478286/140‑1 (MQ=255)                                                                         tgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAAt                                                         <  1:1230025/140‑1 (MQ=255)                                                                             ttACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAt                                                     >  2:776523/1‑140 (MQ=255)                                                                                     gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAAc                                                >  1:412506/1‑137 (MQ=255)                                                                                     gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAAc                                                <  2:412506/137‑1 (MQ=255)                                                                                     gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAt                                             >  1:1123280/1‑140 (MQ=255)                                                                                      cTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTaa                                                           >  2:1113060/1‑125 (MQ=255)                                                                                      cTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTaa                                                           <  1:1113060/125‑1 (MQ=255)                                                                                      cTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAtt                                            >  1:1160808/1‑140 (MQ=255)                                                                                         gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                         <  1:1155802/140‑1 (MQ=255)                                                                                         gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                         <  2:1002403/140‑1 (MQ=255)                                                                                         gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                         <  1:1304660/140‑1 (MQ=255)                                                                                         gggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGa                                         <  2:1123280/140‑1 (MQ=255)                                                                                          ggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGAt                                        <  2:608831/140‑1 (MQ=255)                                                                                          ggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGAt                                        >  2:323505/1‑140 (MQ=255)                                                                                             cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcac                                     <  1:323505/140‑1 (MQ=255)                                                                                              acaAAAAGCTCTTCTGCTGTGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                    <  2:1354154/140‑1 (MQ=255)                                                                                              acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                    <  2:824625/140‑1 (MQ=255)                                                                                              acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                    <  2:61682/140‑1 (MQ=255)                                                                                              acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                    <  1:1295370/140‑1 (MQ=255)                                                                                              acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATcaca                                    <  2:102/140‑1 (MQ=255)                                                                                               caAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCacac                                   <  1:1141751/140‑1 (MQ=255)                                                                                                              ctgcGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATca                                      >  2:946772/1‑122 (MQ=255)                                                                                                              ctgcGCGAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATca                                      <  1:946772/122‑1 (MQ=255)                                                                                                                   cgAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATg                               >  1:253879/1‑124 (MQ=255)                                                                                                                   cgAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATg                               <  2:253879/124‑1 (MQ=255)                                                                                                                   cgAGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGttttt               >  1:1242420/1‑140 (MQ=255)                                                                                                                     aGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTa                        >  1:255267/1‑129 (MQ=255)                                                                                                                     aGTGAAACTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTa                        <  2:255267/129‑1 (MQ=255)                                                                                                                           aCTTAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTg       >  2:496925/1‑140 (MQ=255)                                                                                                                             ttAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg     <  1:114737/140‑1 (MQ=255)                                                                                                                             ttAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg     <  1:496925/140‑1 (MQ=255)                                                                                                                             ttAAATGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAg     <  2:292054/140‑1 (MQ=255)                                                                                                                                aaTGGCGTGATGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCa  >  1:1074314/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   GTCAGCAGGAGTGAACGATTACGGCGGCGGAACAGTTTTAGCCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCA  >  NC_000913/2942380‑2942646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑