BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	466.4 / NA	131	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (73/58); total (73/58)

Only 100 of 132 total aligned reads displayed.

TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844

ttATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCa                                                                                                                                      <  2:1236147/140‑1 (MQ=255)

     gTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGcc                                                                                                                                 >  1:737265/1‑140 (MQ=255)

          gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                            >  2:494791/1‑140 (MQ=255)

           tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                           >  2:1259549/1‑140 (MQ=255)

             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                         >  1:657828/1‑140 (MQ=255)

               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  2:100622/1‑140 (MQ=255)

               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  1:708223/1‑140 (MQ=255)

               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  1:351188/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:552425/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:678675/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:300092/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:902607/1‑140 (MQ=255)

                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    <  2:826088/140‑1 (MQ=255)

                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    <  2:902607/140‑1 (MQ=255)

                     ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                 <  1:1259549/140‑1 (MQ=255)

                     ttGAGCCTCCTGAATAGTGACGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                 <  1:354613/140‑1 (MQ=255)

                          cctcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTg                                                                                                           >  1:456228/1‑140 (MQ=255)

                            tcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGa                                                                                                         <  1:673133/140‑1 (MQ=255)

                             cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAg                                                                                                        >  1:1268171/1‑140 (MQ=255)

                             cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAg                                                                                                        >  1:1061814/1‑140 (MQ=255)

                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGt                                                                                                    <  1:166747/140‑1 (MQ=255)

                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGtt                                                                                               <  1:380048/140‑1 (MQ=255)

                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGtt                                                                                               <  1:494791/140‑1 (MQ=255)

                                       gATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGttt                                                                                              <  2:456228/140‑1 (MQ=255)

                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTa                                                                                             >  2:932122/1‑140 (MQ=255)

                                               ataacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGc                                                                                      >  2:103995/1‑140 (MQ=255)

                                                taacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCa                                                                                     >  2:1354085/1‑140 (MQ=255)

                                                 aacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  2:708223/140‑1 (MQ=255)

                                                 aacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  1:998919/140‑1 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  2:1205921/133‑1 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    >  1:1313095/1‑133 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCAc                                                                                    <  2:1313095/133‑1 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:53668/1‑140 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:385896/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:1175823/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:274574/1‑140 (MQ=255)

                                                               atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:1067649/140‑1 (MQ=255)

                                                               atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:688353/140‑1 (MQ=255)

                                                                tatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:282194/140‑1 (MQ=255)

                                                                tatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:351188/140‑1 (MQ=255)

                                                                  tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                   >  2:453796/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATg                                                                               <  2:564797/127‑1 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:67535/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGT‑ATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgga                                                                 >  2:153464/1‑140 (MQ=255)

                                                                    tCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgga                                                                 >  1:250162/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:211355/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  2:1287421/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  2:454185/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:147307/1‑140 (MQ=255)

                                                                           aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  2:614756/140‑1 (MQ=255)

                                                                            ataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGATGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAtt                                                         >  1:1008509/1‑140 (MQ=255)

                                                                             taatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  1:552425/140‑1 (MQ=255)

                                                                               atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGcc                                                      <  1:953531/140‑1 (MQ=255)

                                                                                tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     >  1:1001811/1‑140 (MQ=255)

                                                                                tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     >  1:552927/1‑140 (MQ=255)

                                                                                tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     >  1:119104/1‑140 (MQ=255)

                                                                                tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     >  1:1198045/1‑140 (MQ=255)

                                                                                tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     >  1:894873/1‑140 (MQ=255)

                                                                                    tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  2:589975/1‑140 (MQ=255)

                                                                                     aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAATCTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  1:1059757/1‑140 (MQ=255)

                                                                                     aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  2:203648/1‑140 (MQ=255)

                                                                                       aTTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTc                                              >  1:871666/1‑140 (MQ=255)

                                                                                        tttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCt                                             <  2:385896/140‑1 (MQ=255)

                                                                                         ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            <  2:368571/140‑1 (MQ=255)

                                                                                            aaaaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        >  1:1109326/1‑125 (MQ=255)

                                                                                            aaaaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  2:1109326/125‑1 (MQ=255)

                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       >  1:937403/1‑140 (MQ=255)

                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       >  1:508602/1‑140 (MQ=255)

                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     <  2:508602/140‑1 (MQ=255)

                                                                                                 gCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGc                                    <  1:932122/140‑1 (MQ=255)

                                                                                                  cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGc                                                    <  2:49523/123‑1 (MQ=255)

                                                                                                  cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGc                                                    >  1:49523/1‑123 (MQ=255)

                                                                                                   aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAATCTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  2:1059757/140‑1 (MQ=255)

                                                                                                   aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  2:53668/140‑1 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       >  2:193737/1‑128 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       <  1:193737/128‑1 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           >  2:289366/1‑140 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:103995/140‑1 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:1001811/140‑1 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:1175823/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:1195076/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:1348989/140‑1 (MQ=255)

                                                                                                              aaaaTGCAAAAAACCAACAAAACCAAAGCAAT‑CCCGGAAAAACTGGCCTTCCCCTGAGGTGGCCGGTTTAGCG‑TGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:823649/139‑1 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           >  2:1323491/1‑135 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:119104/140‑1 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  1:40526/1‑140 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:286314/140‑1 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:59872/1‑123 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     <  2:59872/123‑1 (MQ=255)

                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:100622/140‑1 (MQ=255)

                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:289366/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 >  1:80750/1‑140 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  1:571927/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:737265/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:40526/140‑1 (MQ=255)

                                                                                                                       aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:589975/140‑1 (MQ=255)

                                                                                                                       aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:1354085/140‑1 (MQ=255)

                                                                                                                              accaaaccaGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATAc           <  2:174262/136‑1 (MQ=255)

                                                                                                                              accaaaccaGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATAc           >  1:174262/1‑136 (MQ=255)

                                                                                                                              accaaaccaGAGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       <  2:910401/140‑1 (MQ=255)

TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`