BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	97,750	C→A	R222S (CGC→AGC)	murD →	UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	97,750	0	C	A	100.0%	371.4 / NA	104	R222S (CGC→AGC)	murD	UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (62/42); total (62/42)

Only 100 of 105 total aligned reads displayed.

CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGG  >  NC_000913/97614‑97884

cATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTagcg                                                                                                                                       <  2:852584/140‑1 (MQ=255)

      gAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCgg                                                                                                                               >  1:1138211/1‑140 (MQ=255)

            gACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑g                                                                                                                                  >  2:1397609/1‑131 (MQ=255)

            gACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑g                                                                                                                                  <  1:1397609/131‑1 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGTATGAACg                                                                                                                        >  1:1186187/1‑140 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACg                                                                                                                        >  2:454170/1‑140 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACg                                                                                                                        >  1:1093369/1‑140 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACg                                                                                                                        <  2:325803/140‑1 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACg                                                                                                                        >  2:1383761/1‑140 (MQ=255)

             aCTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACg                                                                                                                        >  1:620256/1‑140 (MQ=255)

                      cATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAg                                                                                                               >  2:1066479/1‑140 (MQ=255)

                        tatGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCt                                                                                                             >  1:1248694/1‑140 (MQ=255)

                           ggATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCttt                                                                                                           >  1:978738/1‑139 (MQ=255)

                           ggATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCttt                                                                                                           <  2:978738/139‑1 (MQ=255)

                           ggATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTg                                                                                                          <  1:1066479/140‑1 (MQ=255)

                             aTCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGaa                                                                                                                          <  1:1197540/122‑1 (MQ=255)

                             aTCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGaa                                                                                                                          >  2:1197540/1‑122 (MQ=255)

                             aTCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGc                                                                                                        >  2:1266148/1‑140 (MQ=255)

                             aTCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGc                                                                                                        >  1:437543/1‑140 (MQ=255)

                               cGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGt                                                                                                      >  1:846174/1‑140 (MQ=255)

                               cGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGt                                                                                                      >  2:502808/1‑140 (MQ=255)

                               cGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGt                                                                                                      <  1:1374419/140‑1 (MQ=255)

                                 cTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCa                                                                                                    >  1:823149/1‑140 (MQ=255)

                                 cTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCa                                                                                                    >  1:97103/1‑140 (MQ=255)

                                  tATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGTGCGCGGATGAACGCTGCGTCAGCTTTGGCGTc                                                                                                     >  2:1379654/1‑140 (MQ=255)

                                  tATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCaa                                                                                                   >  2:472593/1‑140 (MQ=255)

                                    tCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCt                                                                                                             <  1:532425/128‑1 (MQ=255)

                                    tCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCt                                                                                                             >  2:532425/1‑128 (MQ=255)

                                    tCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACa                                                                                                 <  1:502808/140‑1 (MQ=255)

                                        tttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATggg                                                                                             <  2:1138211/140‑1 (MQ=255)

                                         ttGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGt                                                                                            >  1:279460/1‑140 (MQ=255)

                                          tGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTg                                                                                           >  1:1344155/1‑140 (MQ=255)

                                             tttACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACt                                                                                        <  2:620256/140‑1 (MQ=255)

                                                acaacaGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATc                                                                                     >  1:277725/1‑140 (MQ=255)

                                                  aacaGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCAc                                                                                   <  2:1093369/140‑1 (MQ=255)

                                                     aGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTg                                                                                >  2:1185970/1‑140 (MQ=255)

                                                       tATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGaa                                                                              >  1:1213846/1‑140 (MQ=255)

                                                       tATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGaa                                                                              >  1:90943/1‑140 (MQ=255)

                                                       tATCCTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGaa                                                                              >  2:1116584/1‑140 (MQ=255)

                                                            tGCAGCAAACCTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:893566/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTAt                                                                                      <  2:902362/127‑1 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTAt                                                                                      >  1:902362/1‑127 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCt                                                                                 >  2:1269233/1‑132 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCt                                                                                 <  1:1269233/132‑1 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:686412/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:313600/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  2:1175808/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  2:597293/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:1079284/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGC‑‑‑TGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagc                                                                      >  1:1169772/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGCTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:215301/1‑140 (MQ=255)

                                                            tGCAGCAAAACTGCACATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGACGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcatc                                                                         >  1:555980/1‑140 (MQ=255)

                                                              cagcaAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcat                                                                          <  2:887623/137‑1 (MQ=255)

                                                              cagcaAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAatcat                                                                          >  1:887623/1‑137 (MQ=255)

                                                               agcaAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagc                                                                      <  2:1079284/140‑1 (MQ=255)

                                                               agcaAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGAGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagc                                                                      <  2:536546/140‑1 (MQ=255)

                                                                 caAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagcag                                                                    >  1:79626/1‑140 (MQ=255)

                                                                 caAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagcag                                                                    >  2:441094/1‑140 (MQ=255)

                                                                 caAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagcag                                                                    >  1:1427/1‑140 (MQ=255)

                                                                 caAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATcagcag                                                                    >  2:1309183/1‑140 (MQ=255)

                                                                   aaaCTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAggg                                                                  <  2:215301/140‑1 (MQ=255)

                                                                          gcATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAAcc                                                           <  2:97103/140‑1 (MQ=255)

                                                                              ttACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGc                                                       >  1:891250/1‑140 (MQ=255)

                                                                              ttACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGc                                                       >  1:573674/1‑140 (MQ=255)

                                                                              ttACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGc                                                       >  1:574567/1‑140 (MQ=255)

                                                                                aCGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTg                                                     <  2:846174/140‑1 (MQ=255)

                                                                                aCGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTg                                                     <  2:244619/140‑1 (MQ=255)

                                                                                   aaaaCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCgg                                                  >  1:323916/1‑140 (MQ=255)

                                                                                      aCGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGtt                                               <  2:323916/140‑1 (MQ=255)

                                                                                        gcgAAAGTTTGCGTGGTTAATGC‑‑‑TGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAgg                                          <  2:1169772/140‑1 (MQ=255)

                                                                                                ttGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCgaga                                     <  2:1427/140‑1 (MQ=255)

                                                                                                ttGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCgaga                                     <  1:35897/140‑1 (MQ=255)

                                                                                                  gCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGaaa                                   <  1:663822/140‑1 (MQ=255)

                                                                                                     tGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTg                                <  2:755319/140‑1 (MQ=255)

                                                                                                     tGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTg                                <  2:79626/140‑1 (MQ=255)

                                                                                                     tGGTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTg                                <  2:891250/140‑1 (MQ=255)

                                                                                                       gTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCt                              <  1:597293/140‑1 (MQ=255)

                                                                                                       gTTAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCt                              <  2:313600/140‑1 (MQ=255)

                                                                                                         tAATGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGa                            <  2:437543/140‑1 (MQ=255)

                                                                                                          aaTGCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGaa                           <  1:1383761/140‑1 (MQ=255)

                                                                                                             gCTGATGATGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAAtgt                        >  2:948707/1‑140 (MQ=255)

                                                                                                               tgatgaTGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGa                      <  2:893566/140‑1 (MQ=255)

                                                                                                                gatgatGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGaa                     <  1:1185970/140‑1 (MQ=255)

                                                                                                                gatgatGCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGaa                     <  1:454170/140‑1 (MQ=255)

                                                                                                                 atgatgCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGaaa                    >  2:1335314/1‑140 (MQ=255)

                                                                                                                      gCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGAt               >  1:347058/1‑140 (MQ=255)

                                                                                                                      gCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGAt               >  1:1034007/1‑140 (MQ=255)

                                                                                                                      gCCTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGAt               >  1:67428/1‑140 (MQ=255)

                                                                                                                        cTTAACAATGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGa             >  2:606002/1‑140 (MQ=255)

                                                                                                                                tGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTcc     >  1:895139/1‑140 (MQ=255)

                                                                                                                                tGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTcc     >  1:28245/1‑140 (MQ=255)

                                                                                                                                tGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTcc     >  2:1279130/1‑140 (MQ=255)

                                                                                                                                tGCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTcc     >  2:1377917/1‑140 (MQ=255)

                                                                                                                                 gCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCg    >  2:770102/1‑140 (MQ=255)

                                                                                                                                 gCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCg    <  1:606002/140‑1 (MQ=255)

                                                                                                                                 gCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCg    <  1:996417/140‑1 (MQ=255)

                                                                                                                                 gCCGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCg    <  2:895139/140‑1 (MQ=255)

                                                                                                                                   cGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCggg  <  1:1013002/140‑1 (MQ=255)

                                                                                                                                   cGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCggg  <  1:1279130/140‑1 (MQ=255)

                                                                                                                                   cGATTAGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCggg  <  2:1248694/140‑1 (MQ=255)

CATTCTGAACGTGACTGAAGATCATATGGATCGCTATCCGTTTGGTTTACAACAGTATCGTGCAGCAAAACTGCGCATTTACGAAAACGCGAAAGTTTGCGTGGTTAATGCTGATGATGCCTTAACAATGCCGATTCGCGGT‑‑GCGGATGAACGCTGCGTCAGCTTTGGCGTCAACATGGGTGACTATCACCTGAATCATCAGCAGGGCGAAACCTGGCTGCGGGTTAAAGGCGAGAAAGTGCTGAATGTGAAAGAGATGAAACTTTCCGGG  >  NC_000913/97614‑97884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`