Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	824,694	T→G	L22* (TTA→TGA)	ybhQ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	824,694	0	T	G	100.0%	317.7 / NA	86	L22* (TTA→TGA)	ybhQ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/44);  total (42/44)

CGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCGAGCAACGCTGG  >  NC_000913/824567‑824827                                                                                                                                |                                                                                                                                      cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCAGTGTCTGGTAGTGgc                                                                                                                           <  2:539562/140‑1 (MQ=255)        ttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGACAGATTATGTTGCATTGACTGGTAGTGGCGctgctg                                                                                                                    <  1:203949/140‑1 (MQ=255)              aaaGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGgc                                                                                                                           >  2:388019/1‑127 (MQ=255)              aaaGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGgc                                                                                                                           <  1:388019/127‑1 (MQ=255)                aGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTggg                                                                                                            <  1:439067/140‑1 (MQ=255)                    cGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTgg                                                                                                        <  1:1165354/140‑1 (MQ=255)                    cGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTgg                                                                                                        >  2:538981/1‑140 (MQ=255)                          tctGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGt                                                                                                  <  1:771045/140‑1 (MQ=255)                          tctGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGt                                                                                                  >  2:1018069/1‑140 (MQ=255)                           ctGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTa                                                                                                 >  2:468938/1‑140 (MQ=255)                           ctGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTa                                                                                                 <  2:940299/140‑1 (MQ=255)                            tGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTgg                                                                                                        <  1:79515/132‑1 (MQ=255)                            tGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTgg                                                                                                        >  2:79515/1‑132 (MQ=255)                            tGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTaa                                                                                                >  2:777162/1‑140 (MQ=255)                              agtaagtaGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGa                                                                                              >  1:686937/1‑140 (MQ=255)                              agtaagtaGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGa                                                                                              >  1:781130/1‑140 (MQ=255)                                 aagtaGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGActc                                                                                           >  1:416771/1‑140 (MQ=255)                                 aagtaGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGActc                                                                                           <  1:777162/140‑1 (MQ=255)                                   gtaGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTg                                                                                         >  1:948685/1‑140 (MQ=255)                                    taGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGAct                                                                                            >  1:336010/1‑136 (MQ=255)                                    taGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGAct                                                                                            <  2:336010/136‑1 (MQ=255)                                    taGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTgg                                                                                        >  2:22429/1‑140 (MQ=255)                                    taGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTgg                                                                                        >  1:827976/1‑140 (MQ=255)                                      gagaTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGtt                                                                                      >  2:1381906/1‑140 (MQ=255)                                        gaTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCa                                                                                    >  2:545143/1‑140 (MQ=255)                                        gaTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCa                                                                                    >  1:817669/1‑140 (MQ=255)                                        gaTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCa                                                                                    >  2:414471/1‑140 (MQ=255)                                        gaTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCa                                                                                    >  2:653860/1‑140 (MQ=255)                                         aTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTaa                                                                                                >  1:65477/1‑127 (MQ=255)                                         aTAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTaa                                                                                                <  2:65477/127‑1 (MQ=255)                                          tAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACg                                                                                  <  1:22429/140‑1 (MQ=255)                                           aaaTTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGt                                                                                 <  1:545143/140‑1 (MQ=255)                                           aaaTTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGt                                                                                 <  2:827976/140‑1 (MQ=255)                                              ttcttcAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCgg                                                                              >  2:750439/1‑140 (MQ=255)                                                 ttcAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGt                                                                           >  2:1322678/1‑140 (MQ=255)                                                  tcAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTg                                                                          >  2:1087970/1‑140 (MQ=255)                                                         agaAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTAt                                                                   <  1:653860/140‑1 (MQ=255)                                                         agaAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTAt                                                                   <  2:686937/140‑1 (MQ=255)                                                         agaAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTAt                                                                   >  2:1312659/1‑140 (MQ=255)                                                         agaAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTAt                                                                   <  1:802556/140‑1 (MQ=255)                                                         agaAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTAt                                                                   <  1:1381906/140‑1 (MQ=255)                                                           aaGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCg                                                                            <  1:915814/129‑1 (MQ=255)                                                           aaGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCg                                                                            >  2:915814/1‑129 (MQ=255)                                                           aaGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgc                                                                 >  2:1160643/1‑140 (MQ=255)                                                           aaGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgc                                                                 >  2:1225790/1‑140 (MQ=255)                                                           aaGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgc                                                                 >  1:148647/1‑140 (MQ=255)                                                            aGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgcg                                                                <  2:1171507/140‑1 (MQ=255)                                                             gCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgcgc                                                               <  2:148647/140‑1 (MQ=255)                                                             gCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgcgc                                                               <  2:1267257/140‑1 (MQ=255)                                                              ccATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  2:1400782/140‑1 (MQ=255)                                                              ccATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  2:468715/140‑1 (MQ=255)                                                              ccATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  1:1018069/140‑1 (MQ=255)                                                              ccATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  1:538981/140‑1 (MQ=255)                                                              ccATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  2:802674/140‑1 (MQ=255)                                                                aTGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGTGTGGGATTATGCGCACt                                                            >  1:566851/1‑140 (MQ=255)                                                                aTGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgcgc                                                               <  2:514/137‑1 (MQ=255)                                                                aTGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATgcgc                                                               >  1:514/1‑137 (MQ=255)                                                                aTGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACt                                                            <  2:1082810/140‑1 (MQ=255)                                                                aTGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACt                                                            >  2:428335/1‑140 (MQ=255)                                                                   aaGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGA‑‑CTGGTTCACGTCGGCGTGGGATTATgcgc                                                               >  2:1211087/1‑132 (MQ=255)                                                                   aaGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGA‑‑CTGGTTCACGTCGGCGTGGGATTATgcgc                                                               <  1:1211087/132‑1 (MQ=255)                                                                   aaGTGGCAACAACGTGTTCCTGTCGCAACGGGTCTAAGTTGCTGGCAGAATATGTTGCATTGACTCGTAGTGGCCCTGCTGGTGGTCGGCTGAATGAGTAAGACTCTGGTTCCCGTCGGCGTGGGATTATGCGCACTGTa                                                         <  1:1160643/140‑1 (MQ=255)                                                                    aGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTAt                                                        <  2:416771/140‑1 (MQ=255)                                                                        gCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              <  1:902542/130‑1 (MQ=255)                                                                        gCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCa                                                              >  2:902542/1‑130 (MQ=255)                                                                        gCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATtgtg                                                    >  2:1170821/1‑140 (MQ=255)                                                                           acaacGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCa                                                 <  2:948685/140‑1 (MQ=255)                                                                           acaacGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCa                                                 >  1:285592/1‑140 (MQ=255)                                                                           acaacGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCa                                                 <  1:389822/140‑1 (MQ=255)                                                                                    tCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGtga                                        <  1:1225790/140‑1 (MQ=255)                                                                                     cGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGtgat                                       <  1:428335/140‑1 (MQ=255)                                                                                      gtgtCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGtgatg                                      <  2:967355/140‑1 (MQ=255)                                                                                       tgtCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGtgatga                                     >  1:633253/1‑140 (MQ=255)                                                                                          cGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGc                                  >  1:500078/1‑140 (MQ=255)                                                                                           gCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTa                                            <  1:527050/129‑1 (MQ=255)                                                                                           gCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTa                                            >  2:527050/1‑129 (MQ=255)                                                                                             aaCGGGTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTgg                               <  1:414471/140‑1 (MQ=255)                                                                                                  gTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGTGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGttt                          <  2:566851/140‑1 (MQ=255)                                                                                                  gTCTAAGTTGCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGttt                          <  1:26156/140‑1 (MQ=255)                                                                                                           gCTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCAc                 >  2:387889/1‑140 (MQ=255)                                                                                                            cTGGCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCAcc                >  2:1301203/1‑140 (MQ=255)                                                                                                              ggCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCAcccc              >  1:754832/1‑140 (MQ=255)                                                                                                               gCAGATTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCg             >  2:275760/1‑140 (MQ=255)                                                                                                                   aTTATGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCGAGCa         >  2:737666/1‑140 (MQ=255)                                                                                                                      aTGTTGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCGAGCAACg      <  2:817669/140‑1 (MQ=255)                                                                                                                          tGCATTGACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCGAGCAACGCTgg  <  1:1312659/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      CGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTGGGCTGGATGAGTAAGACTCTGGTTCACGTCGGCGTGGGATTATGCGCACTGTATTGTGTCACGGTAGTGATGATGCTGGTGTTTCAGCGCCACCCCGAGCAACGCTGG  >  NC_000913/824567‑824827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑