Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	28.3%	106.6 / 23.5	60	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (42/1);  new base C (0/17);  total (42/18)
Fisher's exact test for biased strand distribution p-value = 4.65e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055                                                                                                                                             |                                                                                                                                          agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGaaaaaaa                                                                                                                                           >  1:1494942/1‑140 (MQ=255)   tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGAATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                         >  1:1412559/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGATATTTGGGGGTT‑ACAGGTAAATACCAGGCAGAAAAAAAcccc                                                                                                                                       >  1:1564990/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  1:654844/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTACATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATAGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                      >  1:86341/1‑140 (MQ=255)           cGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGTGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGt                                                                                                                                 >  1:1007569/1‑140 (MQ=255)                    ccccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGGGGGTTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGaa                                                                                                                        <  2:455642/139‑1 (MQ=255)                       ctgttttATATGATTTATCGGAATGTTTTTCCTTTTCTGCGGGGTTTTAAATCGGAATATTAGTGGGTAAAAGGGGGGGTTATTTGGGGGTT‑AGAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCa                                                                                                                     <  2:1412559/135‑1 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  2:1509725/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  1:1664996/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:1370272/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:767017/1‑140 (MQ=255)                                     ttATCGGCATGATTATCCTTTTCTGCAGGGTTTTTAATGGGACGATTAGGGGGTAAAAGGGGGGGTTATTTGGGGTTT‑AAAGGAAAATTCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                       <  2:440514/140‑1 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  1:90229/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                     >  2:940462/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:557248/1‑140 (MQ=255)                                           gacagATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGGGGGTTTTTTTGGGGGTT‑ACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCt                                                                                                 <  2:852116/136‑1 (MQ=255)                                                aTTATCCGTTTCTGTAGGTTTTAAAATCGGACGATTAGTGGAGGAAGAGAGGGGTTATTGGGGGGTT‑CCAGGTAAATCCAAGGAAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                            <  2:1564990/140‑1 (MQ=255)                                                aTTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                            >  2:1167743/1‑140 (MQ=255)                                                     ccGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGcc                                                                                       >  2:1389106/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:959269/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:1436579/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:1644206/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:1028633/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:852539/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  2:213250/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:1240048/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:160195/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGATGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:430070/1‑140 (MQ=255)                                                           cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgctgtc                                                                                 >  1:852116/1‑135 (MQ=255)                                                                gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            >  1:471867/1‑140 (MQ=255)                                                                gggTTTTAAAGCGGAAAATTAGGGGGAAAAATGGGGGGTTATTGGGGGGTT‑GCAGGAAAATTCCAGGAAAAAAAACACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            <  2:1052115/140‑1 (MQ=255)                                                                   tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                         >  1:1014902/1‑140 (MQ=255)                                                                      ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                      >  2:1600870/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:994179/1‑140 (MQ=255)                                                                              aCGATTAGTGGGTAAAAGGAGGGTTTATTGGGGGGTT‑ACAGGAAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:1644206/140‑1 (MQ=37)                                                                                   gacagggTAAAGTGAGGGTTTTTTTGGGGTTG‑AAGGGTAAATGCCGGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                         <  2:143132/136‑1 (MQ=25)                                                                                          tGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                  >  1:369320/1‑140 (MQ=255)                                                                                              aTGGGGGGTTTTTGGGGGGTT‑ACAGGAAAATTCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                              <  2:1028633/140‑1 (MQ=38)                                                                                                   gggTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGcctgtc                                         >  1:143132/1‑135 (MQ=255)                                                                                                            gggggTTAAAAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                 <  2:654844/140‑1 (MQ=37)                                                                                                              gggTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                              >  1:694171/1‑140 (MQ=255)                                                                                                               ggTT‑ACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             <  2:1014902/140‑1 (MQ=255)                                                                                                                gTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCGGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                            >  1:761963/1‑140 (MQ=255)                                                                                                                 tt‑AAAGGAAAATCCAAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                           <  2:1007569/140‑1 (MQ=37)                                                                                                                  t‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                          >  1:586195/1‑140 (MQ=255)                                                                                                                  t‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                          >  1:1285516/1‑140 (MQ=255)                                                                                                                  t‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                          >  1:341397/1‑140 (MQ=255)                                                                                                                    aCAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                           <  2:790551/138‑1 (MQ=255)                                                                                                                    aCAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                           >  1:790551/1‑138 (MQ=255)                                                                                                                    aCAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  2:313532/1‑140 (MQ=255)                                                                                                                     aagtaaaaTTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  2:767017/135‑1 (MQ=37)                                                                                                                          aaaTTCCAGGCAAAAAACACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  2:471867/140‑1 (MQ=255)                                                                                                                          aaaTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  2:86341/140‑1 (MQ=255)                                                                                                                           aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                  >  2:175854/1‑140 (MQ=255)                                                                                                                           aaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                  >  1:527190/1‑140 (MQ=255)                                                                                                                               ccAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  1:240208/140‑1 (MQ=255)                                                                                                                               ccAGGAAAAAAAAAGCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:369320/140‑1 (MQ=255)                                                                                                                               ccAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCGGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:761963/140‑1 (MQ=255)                                                                                                                               ccAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:1494942/140‑1 (MQ=255)                                                                                                                               ccAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:959269/140‑1 (MQ=255)                                                                                                                                  ggCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           <  1:1389106/140‑1 (MQ=255)                                                                                                                                  ggCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTTTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  2:1250414/1‑140 (MQ=255)                                                                                                                                  ggCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  1:541090/1‑140 (MQ=255)                                                                                                                                   gCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa          >  1:350258/1‑140 (MQ=255)                                                                                                                                       aaaaaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  2:331764/1‑140 (MQ=255)                                                                                                                                           aaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca  <  2:852539/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                          AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTT‑ACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑