Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,563,297	T→C	intergenic (‑221/+37)	ddpX ← / ← dosP	D‑alanyl‑D‑alanine dipeptidase/oxygen‑sensing c‑di‑GMP phosphodiesterase DosP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,563,297	0	T	C	100.0%	261.7 / NA	70	intergenic (‑221/+37)	ddpX/dosP	D‑alanyl‑D‑alanine dipeptidase/oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/31);  total (39/31)

TAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATC  >  NC_000913/1563165‑1563433                                                                                                                                     |                                                                                                                                         tAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGaa                                                                                                                                   >  1:1576482/1‑140 (MQ=255)        atatTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCcgc                                                                                                                            >  1:1006598/1‑140 (MQ=255)        atatTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCcgc                                                                                                                            >  2:302666/1‑140 (MQ=255)                tAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCgg                                                                                                                    >  2:1400598/1‑140 (MQ=255)                tAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCgg                                                                                                                    >  2:1146303/1‑140 (MQ=255)                tAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCgg                                                                                                                    <  1:807451/140‑1 (MQ=255)                     tttGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCgag                                                                                                               >  1:1378283/1‑140 (MQ=255)                       tGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGagag                                                                                                             >  2:580934/1‑140 (MQ=255)                         aTCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGa                                                                                                           <  2:110918/140‑1 (MQ=255)                               aTTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGt                                                                                                     <  2:294776/140‑1 (MQ=255)                                          gTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAg                                                                                          <  2:447022/140‑1 (MQ=255)                                             gTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGATTCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCgg                                                                                       >  2:1093054/1‑140 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAg                                                                                          <  2:496186/136‑1 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAg                                                                                          >  1:496186/1‑136 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGt                                                                                      >  2:826899/1‑140 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGt                                                                                      <  2:1006598/140‑1 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGt                                                                                      >  1:238992/1‑140 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGt                                                                                      >  1:1076344/1‑140 (MQ=255)                                              tGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCAGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGt                                                                                      >  2:281936/1‑140 (MQ=255)                                                     ttGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGGGGAATTTGTCAGATTTTCAGCGGTAACACgc                                                                               >  1:75579/1‑140 (MQ=255)                                                     ttGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACgc                                                                               >  1:534666/1‑140 (MQ=255)                                                        gTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACgctgc                                                                            >  1:223036/1‑140 (MQ=255)                                                             agagGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATc                                                                       >  1:623174/1‑140 (MQ=255)                                                                ggggAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAg                                                                    <  2:1076344/140‑1 (MQ=255)                                                                          aggaaggaATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAAttt                                                          >  1:1121963/1‑140 (MQ=255)                                                                          aggaaggaATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAAttt                                                          >  2:1391716/1‑140 (MQ=255)                                                                               ggaaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcg                                                     <  2:238992/140‑1 (MQ=255)                                                                               ggaaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcg                                                     >  1:116574/1‑140 (MQ=255)                                                                               ggaaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcg                                                     <  1:1093861/140‑1 (MQ=255)                                                                                gaaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcgg                                                    <  1:1222816/140‑1 (MQ=255)                                                                                gaaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcgg                                                    <  2:717467/140‑1 (MQ=255)                                                                                 aaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcggc                                                   >  1:914810/1‑140 (MQ=255)                                                                                 aaTCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTcggc                                                   >  2:609389/1‑140 (MQ=255)                                                                                      cccGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTa                                              >  1:289257/1‑140 (MQ=255)                                                                                          gAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATttct                                                        >  1:82111/1‑126 (MQ=255)                                                                                          gAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATttct                                                        <  2:82111/126‑1 (MQ=255)                                                                                          gAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAgggg                                          >  2:116655/1‑140 (MQ=255)                                                                                             tCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCg                                       <  1:853937/140‑1 (MQ=255)                                                                                              cGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCgg                                      <  1:116655/140‑1 (MQ=255)                                                                                                  gCTGAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCAGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGaaa                                  <  1:281936/140‑1 (MQ=255)                                                                                                     gAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCgg                                      <  2:526455/133‑1 (MQ=255)                                                                                                     gAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCgg                                      >  1:526455/1‑133 (MQ=255)                                                                                                     gAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGa                               >  1:476341/1‑140 (MQ=255)                                                                                                     gAAATCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGa                               >  2:562615/1‑140 (MQ=255)                                                                                                       aaTCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGaaa                             <  1:1093054/140‑1 (MQ=255)                                                                                                       aaTCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGaaa                             <  2:223036/140‑1 (MQ=255)                                                                                                       aaTCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGaaa                             <  2:476341/140‑1 (MQ=255)                                                                                                        aTCACAGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAat                            >  2:1471225/1‑140 (MQ=255)                                                                                                           acaGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATc                         >  2:544204/1‑140 (MQ=255)                                                                                                            caGTATTTAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATcc                        <  1:562615/140‑1 (MQ=255)                                                                                                                 tttAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGaa                   <  1:609389/140‑1 (MQ=255)                                                                                                                  ttAAGTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAAt                  <  1:1400598/140‑1 (MQ=255)                                                                                                                      gTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcg              <  2:1576482/140‑1 (MQ=255)                                                                                                                      gTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcg              <  1:697421/140‑1 (MQ=255)                                                                                                                      gTGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcg              <  1:76478/140‑1 (MQ=255)                                                                                                                       tGACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc             >  1:1612104/1‑140 (MQ=255)                                                                                                                        gACAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgcg            <  1:826899/140‑1 (MQ=255)                                                                                                                         aCAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgcg            >  2:1433832/1‑139 (MQ=255)                                                                                                                         aCAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgcg            <  1:1433832/139‑1 (MQ=255)                                                                                                                         aCAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGa           >  1:267348/1‑140 (MQ=255)                                                                                                                         aCAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGa           >  2:841472/1‑140 (MQ=255)                                                                                                                         aCAGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGa           >  1:474953/1‑140 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGGGGGAAAAGAAATATCCCTGAATAACGCGACa         >  2:1659543/1‑140 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAAt                  <  2:1071096/131‑1 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAAt                  >  1:905445/1‑131 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAAt                  >  1:1071096/1‑131 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAAt                  <  2:905445/131‑1 (MQ=255)                                                                                                                           aGTGTCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACa         >  1:436504/1‑140 (MQ=255)                                                                                                                             tgtCACGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGt       <  1:1036267/140‑1 (MQ=255)                                                                                                                                  cGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATc  <  2:267348/140‑1 (MQ=255)                                                                                                                                  cGTCAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATc  <  1:841472/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TAAAGTTATATTTTTTAACTTTTGATCAACATTTGTGCAGCGTAGTGCAGTTTTGGTGCAAGAGGGGAAGTTAAGGAAGGAATCTCCCGGAATCGTAGCTGAAATCACAGTATTTAAGTGACAGTGTCACGTTAAATGAAAACCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATC  >  NC_000913/1563165‑1563433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑