Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	398,629	C→A	A253D (GCC→GAC)	yaiW →	surface‑exposed outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	398,629	0	C	A	100.0%	192.6 / NA	57	A253D (GCC→GAC)	yaiW	surface‑exposed outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/32);  total (25/32)

GTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAG  >  NC_000913/398498‑398763                                                                                                                                    |                                                                                                                                       gTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAgcggcg                                                                                                                                >  2:1034396/1‑140 (MQ=255)  ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  2:1506887/140‑1 (MQ=255)  ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  1:831597/140‑1 (MQ=255)  ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAAAGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  1:1393818/140‑1 (MQ=255)       gTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGc                                                                                                                          <  1:759905/140‑1 (MQ=255)         ttGGTACTTACCATTTACTGAATTATTCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTg                                                                                                                        <  1:1528806/140‑1 (MQ=255)            gTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCa                                                                                                                     <  2:56947/140‑1 (MQ=255)                      tttACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTg                                                                                                           >  2:1252704/1‑140 (MQ=255)                      tttACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTg                                                                                                           >  1:864171/1‑140 (MQ=255)                         aCTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACgg                                                                                                             >  2:637471/1‑135 (MQ=255)                         aCTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACgg                                                                                                             <  1:637471/135‑1 (MQ=255)                            gAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTaa                                                                                                     <  1:1424706/140‑1 (MQ=255)                              aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                                   >  2:1127802/1‑140 (MQ=255)                              aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                                   >  1:1240887/1‑140 (MQ=255)                                  tCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGtt                                                                                               >  1:1031209/1‑140 (MQ=255)                                  tCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGtt                                                                                               >  1:777548/1‑140 (MQ=255)                                   cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  1:1289384/140‑1 (MQ=255)                                   cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  2:944867/140‑1 (MQ=255)                                   cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  1:867084/140‑1 (MQ=255)                                      gCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGa                                                                                           <  2:171767/140‑1 (MQ=255)                                                    aCCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTa                                                                                      >  1:1599299/1‑131 (MQ=255)                                                    aCCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTa                                                                                      <  2:1599299/131‑1 (MQ=255)                                                     ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                            >  1:219221/1‑140 (MQ=255)                                                     ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                            >  2:1355813/1‑140 (MQ=255)                                                              cGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGa                                                                   >  2:822030/1‑140 (MQ=255)                                                                ttttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAAc                                                                 >  2:1225938/1‑140 (MQ=255)                                                                  ttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAAc                                                                      <  2:909029/133‑1 (MQ=255)                                                                  ttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAAc                                                                      >  1:909029/1‑133 (MQ=255)                                                                   tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  1:884414/1‑140 (MQ=255)                                                                   tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  2:923426/1‑140 (MQ=255)                                                                   tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  1:1243864/1‑140 (MQ=255)                                                                        aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAAcg                                                         <  2:697749/140‑1 (MQ=255)                                                                              aCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCaaa                                                   <  1:1252704/140‑1 (MQ=255)                                                                                    gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                             <  2:258927/124‑1 (MQ=255)                                                                                    gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                             >  1:258927/1‑124 (MQ=255)                                                                                    gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCt                                             >  1:1139545/1‑140 (MQ=255)                                                                                       ggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                          <  2:4989/140‑1 (MQ=255)                                                                                       ggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                          <  2:1240887/140‑1 (MQ=255)                                                                                        gTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaa                                         <  1:1140084/140‑1 (MQ=255)                                                                                            gCCAGCAGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     <  1:1127802/140‑1 (MQ=255)                                                                                              cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg                                   >  2:1229982/1‑140 (MQ=255)                                                                                              cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg                                   >  1:107398/1‑140 (MQ=255)                                                                                              cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGCTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     >  2:86255/1‑138 (MQ=255)                                                                                              cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGCTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     <  1:86255/138‑1 (MQ=255)                                                                                                gCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgggg                                 >  2:1066111/1‑140 (MQ=255)                                                                                                   gTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATg                              >  1:457737/1‑140 (MQ=255)                                                                                                      aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAATCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           <  1:1034396/140‑1 (MQ=255)                                                                                                      aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           >  2:1215658/1‑140 (MQ=255)                                                                                                      aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           <  2:107398/140‑1 (MQ=255)                                                                                                              tttCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGa                   >  2:1153940/1‑140 (MQ=255)                                                                                                                    aaCGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCg             <  1:1153940/140‑1 (MQ=255)                                                                                                                    aaCGCGGTCAGTAAGGACAGCGGAGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCg             <  1:146562/140‑1 (MQ=255)                                                                                                                      cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  2:777548/140‑1 (MQ=255)                                                                                                                      cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  2:884414/140‑1 (MQ=255)                                                                                                                      cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  1:1215658/140‑1 (MQ=255)                                                                                                                       gcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCg          <  2:291561/140‑1 (MQ=255)                                                                                                                         ggTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTc        >  1:928492/1‑140 (MQ=255)                                                                                                                            cAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGt     <  2:1243864/140‑1 (MQ=255)                                                                                                                               tAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTag  <  2:928492/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAG  >  NC_000913/398498‑398763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑