Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	712,298	C→A	G169W (GGG→TGG)	ybfF ←	esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	712,298	0	C	A	100.0%	324.0 / NA	94	G169W (GGG→TGG)	ybfF	esterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (61/33);  total (61/33)

TACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCCGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGA  >  NC_000913/712165‑712434                                                                                                                                      |                                                                                                                                         tACTGCTCGCTAACATACGGAGAATCGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTcaac                                                                                                                                    >  2:1250652/1‑140 (MQ=255)      cTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAg                                                                                                                               >  2:956089/1‑140 (MQ=255)      cTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAg                                                                                                                               >  1:1063509/1‑140 (MQ=255)      cTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAg                                                                                                                               >  2:1060632/1‑140 (MQ=255)         gCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGAtt                                                                                                                            >  1:563828/1‑140 (MQ=255)         gCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGAtt                                                                                                                            >  1:1373619/1‑140 (MQ=255)           tAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTc                                                                                                                          >  1:368350/1‑140 (MQ=255)           tAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTc                                                                                                                          >  2:1121343/1‑140 (MQ=255)              cATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTcagc                                                                                                                       <  2:1346726/140‑1 (MQ=255)              cATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTcagc                                                                                                                       <  1:956089/140‑1 (MQ=255)                tACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTcagcag                                                                                                                     >  2:652847/1‑140 (MQ=255)                     agaATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACt                                                                                                                <  1:4827/140‑1 (MQ=255)                       aaTTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGa                                                                                                              >  2:499221/1‑140 (MQ=255)                         ttGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAAt                                                                                                            <  1:1301316/140‑1 (MQ=255)                         ttGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAAt                                                                                                            >  1:1325021/1‑140 (MQ=255)                          tGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCGCTGCCAGGCAACAAAAGATTTCAGCAGAAACTGAATc                                                                                                           <  2:1329401/140‑1 (MQ=255)                          tGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATc                                                                                                           <  1:499221/140‑1 (MQ=255)                          tGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATc                                                                                                           <  2:1063509/140‑1 (MQ=255)                          tGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATc                                                                                                           <  2:1215736/140‑1 (MQ=255)                          tGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATc                                                                                                           <  2:370987/140‑1 (MQ=255)                           gccgccAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCa                                                                                                          >  1:1024537/1‑140 (MQ=255)                            ccgccAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCAc                                                                                                         >  1:740043/1‑140 (MQ=255)                               ccAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCAcccc                                                                                                      <  1:1424573/140‑1 (MQ=255)                                cAGGGATAAACAGGGCAGGGTTATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCct                                                                                                     <  1:118797/140‑1 (MQ=255)                                cAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCct                                                                                                     <  2:1325021/140‑1 (MQ=255)                                 aGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCctt                                                                                                    >  1:170941/1‑140 (MQ=255)                                  gggATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGATAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCcttc                                                                                                   <  2:740043/140‑1 (MQ=255)                                  gggATAAACAGGCCGGGGTGACCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTATTCCCCCAATACCGGCAGGTTAAGGCGCCCCTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCcttc                                                                                                   <  2:415862/140‑1 (MQ=255)                                    gATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCcttctt                                                                                                 >  2:1663990/1‑140 (MQ=255)                                        aaCAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACACCTTCTTCAtt                                                                                             >  2:1232956/1‑140 (MQ=255)                                         aCAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTa                                                                                            <  1:1663990/140‑1 (MQ=255)                                             ggcagggTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGAt                                                                                        >  2:882847/1‑140 (MQ=255)                                              gcagggTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATg                                                                                       >  2:232080/1‑140 (MQ=255)                                              gcagggTGATCCCATGACGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATg                                                                                       >  2:588563/1‑140 (MQ=255)                                               cagggTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGc                                                                                      <  1:588563/140‑1 (MQ=255)                                                  ggTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTgg                                                                                   >  2:1290896/1‑140 (MQ=255)                                                  ggTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTgg                                                                                   <  2:1373619/140‑1 (MQ=255)                                                       tCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCat                                                                              >  1:200115/1‑140 (MQ=255)                                                                 ggATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgct                                                                    >  1:1445521/1‑140 (MQ=255)                                                                 ggATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATtgctgct                                                                    >  1:1280854/1‑140 (MQ=255)                                                                  gATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATCAAGATGCTGGCGCATTATtgctgctg                                                                   >  1:1249648/1‑140 (MQ=255)                                                                       tctcCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCtgcttgc                                                              >  2:1376728/1‑140 (MQ=255)                                                                         tcCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTg                                                            <  2:1024537/140‑1 (MQ=255)                                                                               ccTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGt                                                      >  2:309829/1‑140 (MQ=255)                                                                                   cAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTtgt                                                  <  2:588587/140‑1 (MQ=255)                                                                                   cAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTtgt                                                  <  2:368350/140‑1 (MQ=255)                                                                                   cAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTtgt                                                  >  1:1668261/1‑140 (MQ=255)                                                                                       atGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTAGCGAGTTTGTGCGt                                              >  1:764976/1‑140 (MQ=255)                                                                                         gCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTg                                                            >  1:1499018/1‑124 (MQ=255)                                                                                         gCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTg                                                            <  2:1499018/124‑1 (MQ=255)                                                                                         gCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTcc                                            >  1:851958/1‑140 (MQ=255)                                                                                         gCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTcc                                            >  2:942560/1‑140 (MQ=255)                                                                                         gCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTcc                                            >  1:512395/1‑140 (MQ=255)                                                                                          cGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCg                                           >  1:1056991/1‑140 (MQ=255)                                                                                          cGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCg                                           >  2:554725/1‑140 (MQ=255)                                                                                             aTACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGAtt                                        >  2:1158378/1‑140 (MQ=255)                                                                                             aTACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGAtt                                        >  2:1120090/1‑140 (MQ=255)                                                                                             aTACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGAtt                                        >  2:759538/1‑140 (MQ=255)                                                                                             aTACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGAtt                                        >  2:1413148/1‑140 (MQ=255)                                                                                             aTACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGAtt                                        >  1:1677084/1‑140 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGc                                                <  1:1480349/127‑1 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGc                                                >  2:1480349/1‑127 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTg                                   >  2:290379/1‑140 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTg                                   >  1:58796/1‑140 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTg                                   <  2:851958/140‑1 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGANNNNNTg                                   >  2:707557/1‑140 (MQ=255)                                                                                                  gATCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTCCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTg                                   >  2:374964/1‑140 (MQ=255)                                                                                                   aTCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGa                                  >  1:1175713/1‑140 (MQ=255)                                                                                                   aTCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGa                                  >  2:648460/1‑140 (MQ=255)                                                                                                   aTCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGa                                  <  2:1280854/140‑1 (MQ=255)                                                                                                    tCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGAc                                 <  1:290379/140‑1 (MQ=255)                                                                                                    tCCCACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTCCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGAc                                 <  1:374964/140‑1 (MQ=255)                                                                                                     cccACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGAcc                                <  1:1084193/140‑1 (MQ=255)                                                                                                     cccACAATACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGAcc                                >  1:1544622/1‑140 (MQ=255)                                                                                                           aTACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTg                          >  1:532232/1‑140 (MQ=255)                                                                                                           aTACCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTg                          >  1:388638/1‑140 (MQ=255)                                                                                                             aCCGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGAt                        >  1:92000/1‑140 (MQ=255)                                                                                                               cGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAg                      <  2:170941/140‑1 (MQ=255)                                                                                                               cGGCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAg                      >  1:247884/1‑140 (MQ=255)                                                                                                                 gCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGcc                    >  2:751750/1‑140 (MQ=255)                                                                                                                 gCACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGcc                    >  2:1500811/1‑140 (MQ=255)                                                                                                                  cACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCg                   >  1:375268/1‑140 (MQ=255)                                                                                                                  cACGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCg                   >  1:952616/1‑140 (MQ=255)                                                                                                                   aCGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGc                  >  2:1133252/1‑140 (MQ=255)                                                                                                                    cGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCa                 >  1:662365/1‑140 (MQ=255)                                                                                                                    cGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCa                 >  1:358780/1‑140 (MQ=255)                                                                                                                    cGTTAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCa                 >  1:1640682/1‑140 (MQ=255)                                                                                                                       tAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAg                      >  2:543165/1‑132 (MQ=255)                                                                                                                       tAAAGCGCCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAg                      <  1:543165/132‑1 (MQ=255)                                                                                                                             gcCACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCtca        <  2:200115/140‑1 (MQ=255)                                                                                                                               cACTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCtcatc      >  1:1580134/1‑140 (MQ=255)                                                                                                                                 cTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTcatcat    >  1:472419/1‑140 (MQ=255)                                                                                                                                 cTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTcatcat    <  1:1250652/140‑1 (MQ=255)                                                                                                                                 cTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTcatcat    <  1:232080/140‑1 (MQ=255)                                                                                                                                 cTCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTcatcat    <  2:92000/140‑1 (MQ=255)                                                                                                                                  tCCCAGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATg   <  1:1060632/140‑1 (MQ=255)                                                                                                                                   cccaGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGa  <  2:1668261/140‑1 (MQ=255)                                                                                                                                   cccaGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTAGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGa  <  2:764976/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TACTGCTCGCTAACATACGGAGAATTGCCGCCAGGGATAAACAGGGCAGGGTGATCCCATGCCGGGATTTTCTCCCAACCTACAATATGCGGATACTGATCCCACAATACCGGCACGTTAAAGCGCCACTCCCCGTCAACAAAAGATTTCAGCAGAAACTGAATCACCCCTTCTTCATTAAGATGCTGGCGCATTATTGCTGCTGCTTGCTGGCGAGTTTGTGCGTCCGATTCACTGACCGCGTTGATAGCCGCAAAAATCTCATCATGA  >  NC_000913/712165‑712434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑