Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	53,257	G→T	L54I (CTC→ATC)	pdxA ←	4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	53,257	0	G	T	100.0%	304.8 / NA	87	L54I (CTC→ATC)	pdxA	4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/39);  total (48/39)

ATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT  >  NC_000913/53129‑53389                                                                                                                                 |                                                                                                                                     aTAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGa                                                                                                                           >  2:277396/1‑140 (MQ=255)  tAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAg                                                                                                                          >  2:448636/1‑140 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGc                                                                                                                                  >  2:472946/1‑131 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGc                                                                                                                                  <  1:472946/131‑1 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAg                                                                                                                          <  1:43410/139‑1 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAg                                                                                                                          >  2:43410/1‑139 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGc                                                                                                                         >  2:1052688/1‑140 (MQ=255)   aaTGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGc                                                                                                                         >  2:458644/1‑140 (MQ=255)     tGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCAt                                                                                                                       >  1:726850/1‑140 (MQ=255)     tGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCAt                                                                                                                       <  1:365718/140‑1 (MQ=255)     tGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGCAGCGTTAATGCGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGCTCGGCAAACCGAGCAt                                                                                                                       >  2:5376/1‑140 (MQ=255)       cccATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCg                                                                                                                     >  1:481443/1‑140 (MQ=255)        ccATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGc                                                                                                                    >  1:278384/1‑140 (MQ=255)          aTTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTg                                                                                                                  >  2:711327/1‑140 (MQ=255)           ttttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGc                                                                                                                 >  2:428842/1‑140 (MQ=255)             ttCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGccc                                                                                                               <  2:278384/140‑1 (MQ=255)               cAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCgg                                                                                                             <  2:302491/140‑1 (MQ=255)               cAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCgg                                                                                                             <  1:803760/140‑1 (MQ=255)                    gCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGt                                                                                                        <  1:697748/140‑1 (MQ=255)                     cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTa                                                                                                       >  1:208665/1‑140 (MQ=255)                     cTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTa                                                                                                       >  2:767047/1‑140 (MQ=255)                      tAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTaa                                                                                                      >  2:486374/1‑140 (MQ=255)                      tAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAATAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTaa                                                                                                      >  1:280071/1‑140 (MQ=255)                       aaCTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAg                                                                                                     <  1:711327/140‑1 (MQ=255)                        aCTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAgg                                                                                                    <  2:726850/140‑1 (MQ=255)                        aCTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAgg                                                                                                    >  2:883073/1‑140 (MQ=255)                             cccGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGt                                                                                               <  1:400829/140‑1 (MQ=255)                                gCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATATGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGc                                                                                            >  2:528772/1‑140 (MQ=255)                                     gACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATATGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCgg                                                                                       <  1:528772/140‑1 (MQ=255)                                     gACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCgg                                                                                       <  2:208665/140‑1 (MQ=255)                                      aCAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGt                                                                                                        >  1:817386/1‑122 (MQ=255)                                      aCAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGt                                                                                                        <  2:817386/122‑1 (MQ=255)                                      aCAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGc                                                                                      >  2:1145018/1‑140 (MQ=255)                                       cAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGca                                                                                     <  1:448636/140‑1 (MQ=255)                                              aCGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCacaaacaa                                                                              <  1:375956/140‑1 (MQ=255)                                                  aGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAg                                                                          >  1:1154061/1‑140 (MQ=255)                                                    cgcgACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGtt                                                                        >  1:1373/1‑140 (MQ=255)                                                    cgcgACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGtt                                                                        >  2:1025956/1‑140 (MQ=255)                                                       gACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGa                                                                     >  2:273354/1‑140 (MQ=255)                                                        aCAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGAc                                                                    <  1:1076976/140‑1 (MQ=255)                                                          aGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCg                                                                  <  1:486374/140‑1 (MQ=255)                                                          aGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCg                                                                  <  1:1025956/140‑1 (MQ=255)                                                            gAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGc                                                                >  1:500797/1‑140 (MQ=255)                                                               gTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCAc                                                             <  1:767047/140‑1 (MQ=255)                                                               gTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCAc                                                             <  2:840880/140‑1 (MQ=255)                                                                tAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACt                                                            <  1:1145018/140‑1 (MQ=255)                                                                   cGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCAc                                                         >  2:675883/1‑140 (MQ=255)                                                                     ttAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGc                                                       >  1:291529/1‑140 (MQ=255)                                                                             cccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                               >  1:1008150/1‑140 (MQ=255)                                                                             cccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                               <  2:500797/140‑1 (MQ=255)                                                                             cccGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                               >  1:426704/1‑140 (MQ=255)                                                                             cccGCAGTTTGCGGTTGTGCAGGGAAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                               >  2:443162/1‑140 (MQ=255)                                                                             cccGCAGTTTGCGGATGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGAGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAg                                               >  2:107366/1‑140 (MQ=255)                                                                                gCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCa                                                 <  2:925793/135‑1 (MQ=255)                                                                                gCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCa                                                 >  1:925793/1‑135 (MQ=255)                                                                                gCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTg                                            <  1:277396/140‑1 (MQ=255)                                                                                 cAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTgg                                           >  1:1121108/1‑140 (MQ=255)                                                                                  aGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTggc                                          >  1:999989/1‑139 (MQ=255)                                                                                  aGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGa                                          <  1:458896/140‑1 (MQ=255)                                                                                            tgtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTc                                <  2:1225671/140‑1 (MQ=255)                                                                                            tgtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTc                                >  1:890262/1‑140 (MQ=255)                                                                                             gtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTcc                               <  1:675883/140‑1 (MQ=255)                                                                                             gtgCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTcc                               >  2:1205509/1‑140 (MQ=255)                                                                                                 aGGGGAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGc                           <  1:458644/140‑1 (MQ=255)                                                                                                   gggAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCa                         >  1:1080994/1‑140 (MQ=255)                                                                                                     gAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAAt                       >  2:200381/1‑140 (MQ=255)                                                                                                     gAGTTGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAAt                       >  1:371476/1‑140 (MQ=255)                                                                                                        ttGGGGGAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATccc                    >  1:660716/1‑140 (MQ=255)                                                                                                             ggAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCcggcgg               >  2:51379/1‑140 (MQ=255)                                                                                                             ggAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCcggcgg               >  2:1202972/1‑140 (MQ=255)                                                                                                              gAATAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCggg              <  1:200381/140‑1 (MQ=255)                                                                                                               aaTAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGc             >  2:1011216/1‑140 (MQ=255)                                                                                                                 tAAGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTc           >  1:321874/1‑140 (MQ=255)                                                                                                                   aGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGc         <  2:1008150/140‑1 (MQ=255)                                                                                                                   aGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGc         <  2:481443/140‑1 (MQ=255)                                                                                                                   aGGGCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCAGGCCAATCCCGGCGGGCTCGc         <  1:383239/140‑1 (MQ=255)                                                                                                                     ggCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg       >  2:444962/1‑140 (MQ=255)                                                                                                                     ggCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg       <  1:1205509/140‑1 (MQ=255)                                                                                                                     ggCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg       <  1:1202972/140‑1 (MQ=255)                                                                                                                     ggCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg       <  2:371476/140‑1 (MQ=255)                                                                                                                     ggCGGAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCg       >  1:1003265/1‑140 (MQ=255)                                                                                                                        ggAGGGTGCTCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGa    <  1:1155197/140‑1 (MQ=255)                                                                                                                         gAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAg   >  2:908381/1‑140 (MQ=255)                                                                                                                         gAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAg   >  2:1180544/1‑140 (MQ=255)                                                                                                                         gAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTAGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAg   >  1:448706/1‑140 (MQ=255)                                                                                                                         gAGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGACAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAg   >  2:741782/1‑140 (MQ=255)                                                                                                                          aGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt  <  1:1180544/140‑1 (MQ=255)                                                                                                                          aGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt  <  2:426704/140‑1 (MQ=255)                                                                                                                          aGGGTGATCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGt  <  1:1052688/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     ATAATGCCCATTTTCAACCGCTAACTGCCCCGCAGTGACAGGTGCACGTAGCGCGACAGGAAGTAGCGTTAATGTGCCCGCAGTTTGCGGTTGTGCAGGGGAGTTGGGGGAATAAGGGCGGAGGGTGAGCGGCAAACCGAGCATCGCTGCCCGGTTGGTAAGGAGAGTGGCATCGGCACAAACAACCAGTTCGACCGGCCACTCACGCTGTGCAAGCTGGACAACTAAGTCCGGGCCAATCCCGGCGGGCTCGCCGGGAGT  >  NC_000913/53129‑53389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑