Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,208,024	A→G	intergenic (‑492/‑123)	purH ← / → rrsE	bifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,208,024	0	A	G	100.0%	201.2 / NA	54	intergenic (‑492/‑123)	purH/rrsE	bifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

TCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTT  >  NC_000913/4207888‑4208123                                                                                                                                         |                                                                                                    tCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCaa                                                                                                  <  2:945608/140‑1 (MQ=255)  cacacaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAAt                                                                                                 >  2:372264/1‑140 (MQ=255)    cacaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCt                                                                                               >  2:466696/1‑140 (MQ=255)    cacaAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCt                                                                                               >  2:362189/1‑140 (MQ=255)          cAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCa                                                                                                   >  2:1024419/1‑130 (MQ=255)           aGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTggg                                                                                        <  1:891876/140‑1 (MQ=255)           aGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTggg                                                                                        <  2:1115716/140‑1 (MQ=255)           aGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTggg                                                                                        <  2:1134388/140‑1 (MQ=255)            gCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGc                                                                                       >  2:283272/1‑140 (MQ=255)             ccGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCa                                                                                      >  2:390486/1‑140 (MQ=255)             ccGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCa                                                                                      >  1:3050/1‑140 (MQ=255)                 ttcggttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCg                                                                                  <  1:372264/140‑1 (MQ=255)                     gttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGa                                                                              >  2:338891/1‑140 (MQ=255)                     gttGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGa                                                                              >  1:506598/1‑140 (MQ=255)                        gAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATAc                                                                           <  1:914271/140‑1 (MQ=255)                         aaGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACg                                                                          <  1:466696/140‑1 (MQ=255)                           gagaAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGa                                                                        >  2:554561/1‑140 (MQ=255)                                         aaTTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCa                                                          <  1:390486/140‑1 (MQ=255)                                             cAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGAc                                                      >  1:1243070/1‑140 (MQ=255)                                                    gACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAAt                                               <  1:635432/140‑1 (MQ=255)                                                     aCTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATg                                              >  1:570005/1‑140 (MQ=255)                                                     aCTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATg                                              >  1:91064/1‑140 (MQ=255)                                                          gAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATAc                                         >  1:547129/1‑140 (MQ=255)                                                          gAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATAc                                         <  2:91064/140‑1 (MQ=255)                                                            aaGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCa                                       <  2:675567/140‑1 (MQ=255)                                                               agGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGt                                    >  2:532112/1‑140 (MQ=255)                                                                 gAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGtct                                  <  1:338891/140‑1 (MQ=255)                                                                 gAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGtct                                  <  1:1070730/140‑1 (MQ=255)                                                                       gTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAagag                            <  2:846766/140‑1 (MQ=255)                                                                          atatACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGa                         <  1:757806/140‑1 (MQ=255)                                                                          atatACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGa                         <  1:554561/140‑1 (MQ=255)                                                                          atatACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACAGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGa                         <  1:234387/140‑1 (MQ=255)                                                                               cGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACg                    <  1:283272/140‑1 (MQ=255)                                                                                gCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGGTACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGt                   >  1:1221769/1‑140 (MQ=255)                                                                                gCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGt                   >  2:1042398/1‑140 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGa                         <  2:330255/129‑1 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGa                         >  1:330255/1‑129 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTc              >  1:1008288/1‑140 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTc              >  1:1232234/1‑140 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTc              >  1:342808/1‑140 (MQ=255)                                                                                     cTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTc              >  1:283289/1‑140 (MQ=255)                                                                                      tCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCa             <  1:532112/140‑1 (MQ=255)                                                                                         cgACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTa          <  2:283289/140‑1 (MQ=255)                                                                                           aCAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg        <  1:1042398/140‑1 (MQ=255)                                                                                           aCAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg        <  1:725261/140‑1 (MQ=255)                                                                                           aCAGTGCACTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg        <  2:1232234/140‑1 (MQ=255)                                                                                               tGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg        <  2:1084423/136‑1 (MQ=38)                                                                                               tGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACg        >  1:1084423/1‑136 (MQ=38)                                                                                               tGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt    >  1:138964/1‑140 (MQ=38)                                                                                               tGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGt    >  1:1255890/1‑140 (MQ=38)                                                                                                 cgcTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGttt  >  2:664651/1‑140 (MQ=37)                                                                                                 cgcTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGttt  >  2:874999/1‑140 (MQ=37)                                                                                                  gcTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAAt                <  1:692487/125‑1 (MQ=25)                                                                                                  gcTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGGCAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAAt                >  2:692487/1‑125 (MQ=25)                                                                                                                                         |                                                                                                    TCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTT  >  NC_000913/4207888‑4208123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑