BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	443.6 / NA	125	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (70/55); total (70/55)

Only 100 of 126 total aligned reads displayed.

TGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261577‑4261844

tGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATg                                                                                                                                    >  2:41147/1‑140 (MQ=255)

  gTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGcc                                                                                                                                  <  1:836044/140‑1 (MQ=255)

    ataGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTg                                                                                                                                >  1:1066079/1‑140 (MQ=255)

    ataGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTg                                                                                                                                >  1:823904/1‑140 (MQ=255)

    ataGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTg                                                                                                                                >  1:394748/1‑140 (MQ=255)

     taGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                               >  1:941256/1‑140 (MQ=255)

          ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                          >  1:1007980/1‑140 (MQ=255)

          ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                          >  2:497252/1‑140 (MQ=255)

          ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                          >  1:703980/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  2:906510/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  2:663960/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  2:1035204/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  1:275661/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  1:165050/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       >  1:1181556/1‑140 (MQ=255)

             cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                       <  1:1035204/140‑1 (MQ=255)

               cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAGCCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     <  1:569755/140‑1 (MQ=255)

               cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     <  1:663960/140‑1 (MQ=255)

               cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     <  1:1260980/140‑1 (MQ=255)

               cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     >  2:11281/1‑140 (MQ=255)

                tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                    <  2:713222/140‑1 (MQ=255)

                    gAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑Ca                                                                                                              <  1:395189/140‑1 (MQ=255)

                         tcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGa                                                                                                         >  1:1160286/1‑140 (MQ=255)

                          cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAg                                                                                                        >  2:1126806/1‑140 (MQ=255)

                           ctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGt                                                                                                       <  1:41147/140‑1 (MQ=255)

                            tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     <  1:77149/127‑1 (MQ=255)

                            tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                     >  2:77149/1‑127 (MQ=255)

                                     aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCATGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTa                                                                                             >  1:616310/1‑140 (MQ=255)

                                     aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTa                                                                                             >  1:112078/1‑140 (MQ=255)

                                        cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCg                                                                                          >  1:315726/1‑140 (MQ=255)

                                          gAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGtt                                                                                        >  2:291303/1‑140 (MQ=255)

                                           aaTAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTg                                                                                       >  2:259878/1‑140 (MQ=255)

                                               acataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCAcc                                                                                   <  2:823904/140‑1 (MQ=255)

                                               acataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCAcc                                                                                   <  2:394748/140‑1 (MQ=255)

                                                cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCa                                                                                  >  2:574268/1‑140 (MQ=255)

                                                cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCa                                                                                  >  2:546460/1‑140 (MQ=255)

                                                     ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACACTGGAGTGGTCGGTTTAGCGTTGCACCAATg                                                                               >  2:175567/1‑140 (MQ=255)

                                                     ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:246992/1‑140 (MQ=255)

                                                     ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:568194/1‑140 (MQ=255)

                                                        cATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAGTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGG‑TTAGCGTTGCAc                                                                                    >  2:88283/1‑129 (MQ=255)

                                                         aTGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGAc                                                                         <  2:778897/140‑1 (MQ=255)

                                                          tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:343370/1‑140 (MQ=255)

                                                           gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                       >  2:1037270/1‑140 (MQ=255)

                                                            atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  1:259878/140‑1 (MQ=255)

                                                            atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:45946/140‑1 (MQ=255)

                                                               tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                   >  1:1002698/1‑140 (MQ=255)

                                                                atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:380849/1‑140 (MQ=255)

                                                                atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:327046/1‑140 (MQ=255)

                                                                 tCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgga                                                                 >  1:1074997/1‑140 (MQ=255)

                                                                    aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:313207/132‑1 (MQ=255)

                                                                    aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  2:177595/1‑140 (MQ=255)

                                                                    aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:1197911/1‑140 (MQ=255)

                                                                    aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:959772/1‑140 (MQ=255)

                                                                     tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  2:151748/1‑140 (MQ=255)

                                                                     tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  2:712267/1‑140 (MQ=255)

                                                                        aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCGATGCTCGACTGGACGGACAGACAt                                                          <  1:39321/140‑1 (MQ=255)

                                                                        aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          >  1:1024495/1‑140 (MQ=255)

                                                                        aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  1:574268/140‑1 (MQ=255)

                                                                         ataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAtt                                                         >  2:192521/1‑140 (MQ=255)

                                                                          taatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        >  2:755766/1‑140 (MQ=255)

                                                                          taatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  1:712267/140‑1 (MQ=255)

                                                                             tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGc                                                       <  1:175567/140‑1 (MQ=255)

                                                                              ctctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGc                                                    >  1:1097744/1‑140 (MQ=255)

                                                                               tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   >  2:103557/1‑140 (MQ=255)

                                                                               tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   <  1:151748/140‑1 (MQ=255)

                                                                               tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   >  2:1210885/1‑140 (MQ=255)

                                                                                 tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  2:1240934/1‑140 (MQ=255)

                                                                                  aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                >  1:1030254/1‑140 (MQ=255)

                                                                                      ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  2:52234/1‑140 (MQ=255)

                                                                                        gAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGc                                          <  1:1188438/140‑1 (MQ=255)

                                                                                              gCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGt                                        >  2:1077081/1‑136 (MQ=255)

                                                                                              gCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGAACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGc                                    >  1:50239/1‑140 (MQ=255)

                                                                                               cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑GACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCt                                   <  2:343370/140‑1 (MQ=255)

                                                                                                aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  2:1181556/140‑1 (MQ=255)

                                                                                                  ggTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTc                                >  1:349238/1‑140 (MQ=255)

                                                                                                       ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:906510/140‑1 (MQ=255)

                                                                                                       ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:52234/140‑1 (MQ=255)

                                                                                                       ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:1160286/140‑1 (MQ=255)

                                                                                                         cTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAAt                         >  1:254523/1‑140 (MQ=255)

                                                                                                          tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:251789/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:374535/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:170036/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:995920/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:576867/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:291303/140‑1 (MQ=255)

                                                                                                            aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  1:573605/1‑140 (MQ=255)

                                                                                                              aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  2:1081112/1‑140 (MQ=255)

                                                                                                              aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  2:1100638/1‑140 (MQ=255)

                                                                                                              aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  1:471353/1‑140 (MQ=255)

                                                                                                               tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:471353/140‑1 (MQ=255)

                                                                                                               tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:497252/140‑1 (MQ=255)

                                                                                                               tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:1037270/140‑1 (MQ=255)

                                                                                                                 cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 >  2:881199/1‑140 (MQ=255)

                                                                                                                    aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:1081112/140‑1 (MQ=255)

                                                                                                                    aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:1126806/140‑1 (MQ=255)

                                                                                                                      aaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGtata            <  2:1097744/140‑1 (MQ=255)

                                                                                                                                accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  1:717954/140‑1 (MQ=255)

                                                                                                                                accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:1030254/140‑1 (MQ=255)

                                                                                                                                accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  1:177595/140‑1 (MQ=255)

                                                                                                                                accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:1197911/140‑1 (MQ=255)

TGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261577‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`