Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,828	G→T	intergenic (‑148/‑102)	yjiH ← / → kptA	Gate family protein YjiH/RNA 2'‑phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,828	0	G	T	100.0%	261.3 / NA	74	intergenic (‑148/‑102)	yjiH/kptA	Gate family protein YjiH/RNA 2'‑phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/40);  total (34/40)

ATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGAT  >  NC_000913/4560691‑4560962                                                                                                                                          |                                                                                                                                       aTTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCtttg                                                                                                                                      >  2:1212115/1‑140 (MQ=255) aTTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCtttg                                                                                                                                      >  2:1038422/1‑140 (MQ=255) aTTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCtttg                                                                                                                                      >  1:987973/1‑140 (MQ=255) aTTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAACTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCtttg                                                                                                                                      >  1:958147/1‑140 (MQ=255)   tGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCtttgtg                                                                                                                                    >  1:159963/1‑140 (MQ=255)      ttttttGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGaa                                                                                                                                 <  1:709366/140‑1 (MQ=255)       tttttGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGaaa                                                                                                                                <  1:305088/140‑1 (MQ=255)            gtgtttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  1:1239525/140‑1 (MQ=255)            gtgtttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  2:159963/140‑1 (MQ=255)            gtgtttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  2:476592/140‑1 (MQ=255)            gtgtttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  1:1079760/140‑1 (MQ=255)            gtgtttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  1:1038422/140‑1 (MQ=255)               tttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTg                                                                                                                        >  1:600806/1‑140 (MQ=255)               tttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTg                                                                                                                        >  1:495788/1‑140 (MQ=255)               tttgtgtGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTg                                                                                                                        >  1:1016177/1‑140 (MQ=255)                 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:628940/140‑1 (MQ=255)                 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:650649/140‑1 (MQ=255)                 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  2:428137/140‑1 (MQ=255)                 tgtgtgTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:23294/140‑1 (MQ=255)                   tgtgtTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      >  2:422105/1‑138 (MQ=255)                   tgtgtTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:422105/138‑1 (MQ=255)                      gtTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGa                                                                                                                 <  1:20921/140‑1 (MQ=255)                       ttGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAACTGCAAGTGTGGTTCTGGTTATCCGTTTTCTGCATAGCGGATAAATTACGCTCTGTGGAAAAGGCTCTGCGCAGGAt                                                                                                                >  2:628940/1‑140 (MQ=255)                        tGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGAtt                                                                                                               <  1:798128/140‑1 (MQ=255)                         gCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      >  2:1181913/1‑132 (MQ=255)                         gCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      >  2:1090524/1‑132 (MQ=255)                         gCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:1181913/132‑1 (MQ=255)                         gCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTgcg                                                                                                                      <  1:1090524/132‑1 (MQ=255)                            tGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           <  2:578786/124‑1 (MQ=255)                            tGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGct                                                                                                                           >  1:578786/1‑124 (MQ=255)                              aGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTg                                                                                                         >  2:1125615/1‑140 (MQ=255)                                 cAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGAtt                                                                                                               >  2:691637/1‑131 (MQ=255)                                 cAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGAtt                                                                                                               <  1:691637/131‑1 (MQ=255)                                    gAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGaaa                                                                                                   >  2:267609/1‑140 (MQ=255)                                         ttCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCa                                                                                              >  1:1108046/1‑140 (MQ=255)                                           ccTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATa                                                                                            <  2:495788/140‑1 (MQ=255)                                           ccTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATa                                                                                            <  2:600806/140‑1 (MQ=255)                                              ggAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAAc                                                                                         >  1:112591/1‑140 (MQ=255)                                                           cTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGa                                                                            <  1:667171/140‑1 (MQ=255)                                                              aTCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACAt                                                                         >  2:940653/1‑140 (MQ=255)                                                                 aGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAg                                                                      <  1:1125615/140‑1 (MQ=255)                                                                   ccATGCGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGTTTTGGGGAAAAGGCTCCGCTCAGGATTGAGCTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGta                                                                    >  2:28609/1‑140 (MQ=255)                                                                     aTGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACAt                                                                         <  1:381640/133‑1 (MQ=255)                                                                     aTGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACAt                                                                         >  2:381640/1‑133 (MQ=255)                                                                     aTGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaa                                                                  >  1:959091/1‑140 (MQ=255)                                                                      tgtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaat                                                                 >  1:100083/1‑140 (MQ=255)                                                                      tgtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaat                                                                 >  1:536914/1‑140 (MQ=255)                                                                       gtgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaata                                                                >  1:1120493/1‑140 (MQ=255)                                                                        tgGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGtaaataa                                                               >  2:131250/1‑140 (MQ=255)                                                                          ggTATGGGGAAAATGCAAGTATGTTTCTGGCTTTGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATaaaa                                                             >  1:204246/1‑140 (MQ=255)                                                                          ggTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATaaaa                                                             <  2:568440/140‑1 (MQ=255)                                                                             aTGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACa                                                          >  2:1138214/1‑140 (MQ=255)                                                                             aTGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACa                                                          >  2:802990/1‑140 (MQ=255)                                                                              tGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAg                                                         >  1:533881/1‑140 (MQ=255)                                                                               ggggAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGa                                                        <  2:100083/140‑1 (MQ=255)                                                                                gggAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGaa                                                       >  2:782003/1‑140 (MQ=255)                                                                                      aTGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCa                                                 <  2:1208092/140‑1 (MQ=255)                                                                                              aTGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGaa                                         <  2:112591/140‑1 (MQ=255)                                                                                                     tGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACgaga                                  <  1:374368/140‑1 (MQ=255)                                                                                                     tGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACgaga                                  <  2:536914/140‑1 (MQ=255)                                                                                                     tGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACgaga                                  <  2:533881/140‑1 (MQ=255)                                                                                                      ggCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGAt                                 <  1:1212115/140‑1 (MQ=255)                                                                                                       gCTTTGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATg                                <  2:204246/140‑1 (MQ=255)                                                                                                       gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATg                                >  2:732035/1‑140 (MQ=255)                                                                                                       gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATg                                >  1:267566/1‑140 (MQ=255)                                                                                                       gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATg                                >  1:543187/1‑140 (MQ=255)                                                                                                       gCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAAAAGAAAATCCAGGGACGAAAACGAGATg                                >  1:1262518/1‑140 (MQ=255)                                                                                                        cTATGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATgg                               <  1:782003/140‑1 (MQ=255)                                                                                                          aTGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATTAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCa                             <  1:267609/140‑1 (MQ=255)                                                                                                           tGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGaaaa                                       <  2:365206/129‑1 (MQ=255)                                                                                                           tGCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGaaaa                                       >  1:365206/1‑129 (MQ=255)                                                                                                            gCGTTTTTTGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCACTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCaaa                           >  1:482397/1‑140 (MQ=255)                                                                                                                 ttttGCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACa                      <  2:1120493/140‑1 (MQ=255)                                                                                                                     gCATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGa                  <  1:1138214/140‑1 (MQ=255)                                                                                                                      cATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTa                        <  2:819183/133‑1 (MQ=255)                                                                                                                      cATAGCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTa                        >  1:819183/1‑133 (MQ=255)                                                                                                                          gCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACaa                     >  1:176926/1‑132 (MQ=255)                                                                                                                          gCGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACaa                     <  2:176926/132‑1 (MQ=255)                                                                                                                           cGGAAAAATTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAaga            >  2:1146424/1‑140 (MQ=255)                                                                                                                                aaaTTACGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTgg       <  2:1016177/140‑1 (MQ=255)                                                                                                                                     aCGCTTTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGAt  >  2:733354/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTGTTTTTTTGTGTTTGTGTGTTGCTTGAGCCAGGAAAATTCCTGGAACTGAAAAACCTTATCAGCCATGTGGGTATGGGGAAAATGCAAGTATGTTTCTGGCTATGCGTTTTTTGCATAGCGGAAAAATTACGCTGTGTGGAAAAGGCTCTGCGCAGGATTGAGTTGCAGAAAATTCATAAACCAAGTGACAATGACATCAGTAAATAAAAACAGAAAATCCAGGGACGAAAACGAGATGGCAAAGTACAACGAAAAAGAGCTGGCGGAT  >  NC_000913/4560691‑4560962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑