Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,136	C→A	R120L (CGT→CTT)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,136	0	C	A	100.0%	226.9 / NA	64	R120L (CGT→CTT)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/34);  total (30/34)

TTAACCACGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGG  >  NC_000913/612001‑612272                                                                                                                                        |                                                                                                                                         ttAACCACGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCAc                                                                                                                                      <  1:9595/140‑1 (MQ=255)  tAACCACGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCAcc                                                                                                                                     >  1:504270/1‑140 (MQ=255)   aaCCACGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCg                                                                                                                                    >  2:52550/1‑140 (MQ=255)       aCGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGtt                                                                                                                                >  2:50330/1‑140 (MQ=255)        cgccgcCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCAc                                                                                                                                      >  1:1099374/1‑133 (MQ=255)        cgccgcCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCAc                                                                                                                                      <  2:1099374/133‑1 (MQ=255)        cgccgcCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTa                                                                                                                               >  2:841975/1‑140 (MQ=255)        cgccgcCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTa                                                                                                                               >  1:495253/1‑140 (MQ=255)         gccgccCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTAc                                                                                                                              >  2:442036/1‑140 (MQ=255)           cgcccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTAc                                                                                                                              <  1:750567/138‑1 (MQ=255)           cgcccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTAc                                                                                                                              >  2:750567/1‑138 (MQ=255)           cgcccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACgg                                                                                                                            <  2:177120/140‑1 (MQ=255)           cgcccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACgg                                                                                                                            <  2:1031372/140‑1 (MQ=255)             cccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGgct                                                                                                                          >  1:892775/1‑140 (MQ=255)             cccgccGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGgct                                                                                                                          <  2:75166/140‑1 (MQ=255)               cgccgcgccgTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGgctgc                                                                                                                        <  1:841975/140‑1 (MQ=255)                gccgcgccgTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGgctgct                                                                                                                       >  2:254055/1‑140 (MQ=255)                     gccgTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCg                                                                                                                  >  2:821626/1‑140 (MQ=255)                     gccgTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCg                                                                                                                  >  1:169353/1‑140 (MQ=255)                       cgTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGc                                                                                                                <  1:679496/140‑1 (MQ=255)                              aTAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGt                                                                                                         <  1:50330/140‑1 (MQ=255)                              aTAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGt                                                                                                         <  1:335839/140‑1 (MQ=255)                              aTAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGt                                                                                                         <  1:146924/140‑1 (MQ=255)                                            ccGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGc                                                                                           <  2:892775/140‑1 (MQ=255)                                                   aCGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTATGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGtttt                                                                                    >  1:140090/1‑140 (MQ=255)                                                    cGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTc                                                                                   <  2:495253/140‑1 (MQ=255)                                                                  aCGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACcgc                                                                     <  1:442036/140‑1 (MQ=255)                                                                   cGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACcgcg                                                                    >  1:553265/1‑140 (MQ=255)                                                                   cGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACcgc                                                                     <  1:907609/139‑1 (MQ=255)                                                                   cGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACcgc                                                                     >  2:907609/1‑139 (MQ=255)                                                                    gTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGa                                                                   <  1:254055/140‑1 (MQ=255)                                                                     ttCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGaa                                                                  <  1:357721/140‑1 (MQ=255)                                                                      tCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAat                                                                 >  1:56665/1‑140 (MQ=255)                                                                      tCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAat                                                                 >  1:230233/1‑140 (MQ=255)                                                                         aTCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATc                                                              >  2:272619/1‑140 (MQ=255)                                                                              ttCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCt                                                         >  1:343580/1‑140 (MQ=255)                                                                               tCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTATGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTg                                                        <  2:140090/140‑1 (MQ=255)                                                                               tCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTg                                                        <  1:821626/140‑1 (MQ=255)                                                                               tCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTg                                                        <  2:343580/140‑1 (MQ=255)                                                                                cAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGt                                                       <  2:169353/140‑1 (MQ=255)                                                                                    tGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGt                                                   <  1:194599/140‑1 (MQ=255)                                                                                      gCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTa                                                 >  1:1047890/1‑140 (MQ=255)                                                                                              gAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACg                                         <  2:1047890/140‑1 (MQ=255)                                                                                              gAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACg                                         <  1:52550/140‑1 (MQ=255)                                                                                                 gTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTg                                      >  2:928533/1‑140 (MQ=255)                                                                                                 gTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTg                                      <  2:440065/140‑1 (MQ=255)                                                                                                   ttCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAc                                    >  2:166348/2‑140 (MQ=255)                                                                                                   aTCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAc                                    <  1:272619/140‑1 (MQ=255)                                                                                                   aTCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAc                                    <  1:662579/140‑1 (MQ=255)                                                                                                    tCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAcc                                   >  2:1100105/1‑140 (MQ=255)                                                                                                    tCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAcc                                   >  1:1233694/1‑140 (MQ=255)                                                                                                    tCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGAcc                                   >  1:81918/1‑140 (MQ=255)                                                                                                        cacGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACg                                         >  1:536343/1‑130 (MQ=255)                                                                                                        cacGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACg                                         <  2:536343/130‑1 (MQ=255)                                                                                                        cacGGGCATCGCGCTCCCCACGCCAGCCCTGAAGCACCGACTTACGGCTGCTTACCGGCTTGCCCTCAATCAAAAAAAGCGGGTTTTCCAGACCCACACCGCTAGAGTCACTCTCCAGGTTATTCCCACGCTGACCACtg                               >  2:525660/1‑140 (MQ=255)                                                                                                          cGGGTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACtggt                             >  1:1043394/1‑140 (MQ=255)                                                                                                            ggTATCGCGCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACtggtgg                           >  1:289061/1‑140 (MQ=255)                                                                                                                 cgcgCTCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTa                      <  1:801705/140‑1 (MQ=255)                                                                                                                      tCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGt                 <  2:289061/140‑1 (MQ=255)                                                                                                                      tCGCCACGCCAGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGt                 <  2:230233/140‑1 (MQ=255)                                                                                                                       cgccacgccaGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACt                                <  2:460433/124‑1 (MQ=255)                                                                                                                       cgccacgccaGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACt                                >  1:460433/1‑124 (MQ=255)                                                                                                                       cgccacgccaGCCCTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTc                >  1:924039/1‑140 (MQ=255)                                                                                                                                  cccTGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGcc     <  2:691190/140‑1 (MQ=255)                                                                                                                                     tGAAGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTgg  <  1:528815/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTAACCACGCCGCCCGCCGCGCCGTTGCCATAACGCGCAGCTGCCGGACCACGCAGAACTTCAATACGTTCAATCATTTCAGGTGGCACCCAGGAAGTATCACCACGGGTATCGCGCTCGCCACGCCAGCCCTGACGCACCGAGTTACGGCTGCTTACCGGCTTGCCGTCAATCAAAATCAGCGTGTTTTCCGGACCCATACCGCGAATATCAATCTGTCGGTTATTCCCACGCTGACCACTGGTGGAGTTACCGGTCAGGTTAACGCCTGG  >  NC_000913/612001‑612272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑