Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,713,207	C→A	R147S (CGT→AGT)	dtpA →	dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,713,207	0	C	A	100.0%	124.3 / NA	37	R147S (CGT→AGT)	dtpA	dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/23);  total (14/23)

CTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGCGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCA  >  NC_000913/1713076‑1713345                                                                                                                                    |                                                                                                                                           ctctGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGAc                                                                                                                                    <  2:518264/140‑1 (MQ=255)    tGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGt                                                                                                                                 >  2:1026494/1‑140 (MQ=255)      gTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGAc                                                                                                                                    <  2:472809/135‑1 (MQ=255)      gTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGAc                                                                                                                                    >  1:472809/1‑135 (MQ=255)      gTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGc                                                                                                                               >  1:682249/1‑140 (MQ=255)      gTTGCCTGGTCTGGTCACGACGCCCGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGc                                                                                                                               >  1:882565/1‑140 (MQ=255)         gCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCAtt                                                                                                                            <  1:1039027/140‑1 (MQ=255)         gCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCAtt                                                                                                                            >  2:715941/1‑140 (MQ=255)          ccTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTc                                                                                                                           >  2:217303/1‑140 (MQ=255)           ctggtctggtCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCa                                                                                                                          <  1:491465/140‑1 (MQ=255)           ctggtctggtCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCa                                                                                                                          <  2:569260/140‑1 (MQ=255)           ctggtctggtCACGACGCCCGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCa                                                                                                                          <  2:882565/140‑1 (MQ=255)             ggtctggtcACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCAcc                                                                                                                        <  1:320484/140‑1 (MQ=255)             ggtctggtcACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCAcc                                                                                                                        <  2:735727/140‑1 (MQ=255)              gtctggtcACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCa                                                                                                                       <  2:475293/140‑1 (MQ=255)                ctggtcACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATg                                                                                                                     <  2:981377/140‑1 (MQ=255)                ctggtcACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATg                                                                                                                     <  1:246276/140‑1 (MQ=255)                      acgacgCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGtactac                                                                                                               <  1:1026494/140‑1 (MQ=255)                          cgCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGt                                                                                                           <  1:837694/140‑1 (MQ=255)                          cgCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGt                                                                                                           <  2:1076322/140‑1 (MQ=255)                                          atGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGctc                                                                                           <  1:1101509/140‑1 (MQ=255)                                                    cggcTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTttcttc                                                                                    >  1:377763/1‑137 (MQ=255)                                                    cggcTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTttcttc                                                                                    <  2:377763/137‑1 (MQ=255)                                                      gcTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTAt                                                                               <  1:474242/140‑1 (MQ=255)                                                      gcTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTAt                                                                               <  1:715941/140‑1 (MQ=255)                                                        tATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGa                                                                             >  1:689825/1‑140 (MQ=255)                                                             cggtcggtAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCt                                                                        <  2:682249/140‑1 (MQ=255)                                                              ggtcggtAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTa                                                                       >  1:166128/1‑140 (MQ=255)                                                                            tGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGcc                                                         >  1:294042/1‑140 (MQ=255)                                                                              tttAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCcgc                                                       >  1:527483/1‑140 (MQ=255)                                                                              tttAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCcgc                                                       >  2:599785/1‑140 (MQ=255)                                                                                  aaGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGaaa                                                   <  2:888693/140‑1 (MQ=255)                                                                                                      cTTTCTACATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGttgcgtt                               <  2:523609/140‑1 (MQ=255)                                                                                                             cATGCTATGAGAAAAACGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTg                        <  1:217303/140‑1 (MQ=255)                                                                                                                           aaCGACCCGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCct          >  1:526565/1‑140 (MQ=255)                                                                                                                                 ccGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAg                      <  2:351811/122‑1 (MQ=255)                                                                                                                                 ccGAGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAg                      >  1:351811/1‑122 (MQ=255)                                                                                                                                   gcgTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCa  <  1:927572/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                           CTCTGGTTGCCTGGTCTGGTCACGACGCCGGTATCGTTTATATGGGTATGGCGGCTATTGCGGTCGGTAACGGCCTGTTTAAAGCTAACCCGTCTTCTCTGCTTTCTACATGCTATGAGAAAAACGACCCGCGTCTGGACGGTGCATTCACCATGTACTACATGTCCGTCAACATCGGCTCTTTCTTCTCTATGATTGCTACGCCGTGGCTGGCCGCGAAATACGGCTGGAGTGTTGCGTTTGCGTTGAGCGTTGTAGGCCTGCTGATCA  >  NC_000913/1713076‑1713345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑