Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	372,640	0	T	G	24.7%	167.8 / 15.1	74	L176V (TTA→GTA)	mhpD	2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (13/42);  new base G (18/0);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 3.75e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779                                                                                                                                            |                                                                                                                                             cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                              >  1:1020839/1‑140 (MQ=255) cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGt                                                                                                                                              <  1:702717/140‑1 (MQ=255)  aTTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGtt                                                                                                                                             <  2:761417/140‑1 (MQ=255)    tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  2:1161594/140‑1 (MQ=255)    tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  2:598327/140‑1 (MQ=255)    tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAg                                                                                                                                           <  1:147404/140‑1 (MQ=255)        tGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCt                                                                                                                                      >  1:619846/1‑140 (MQ=255)         gggTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCGACGTCCGGCGGGGGTAGG‑CCTg                                                                                                                                     >  2:62837/1‑140 (MQ=255)          ggTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGa                                                                                                                                    >  1:404735/1‑140 (MQ=255)           gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGGCCCGCGCAACGTCCGGCGGGGTTAGA‑CCTGaa                                                                                                                                   >  1:277949/1‑140 (MQ=255)                tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACt                                                                                                                              <  2:1599837/140‑1 (MQ=255)                 ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTg                                                                                                                             <  1:859254/140‑1 (MQ=255)                 ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTg                                                                                                                             <  1:62837/140‑1 (MQ=255)                 ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCCGAAAAAATg                                                                                                                             >  2:1697841/1‑140 (MQ=255)                 ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGCGCAACGTCCGGCGGGGGTTGA‑CCCGAAAAAATg                                                                                                                             >  2:637993/1‑140 (MQ=255)                   ggCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTgcg                                                                                                                           <  2:1020839/140‑1 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTgcgc                                                                                                                          >  1:1455578/1‑140 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTgcgc                                                                                                                          <  2:1455578/140‑1 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTgcgc                                                                                                                          <  1:369373/140‑1 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTgcgc                                                                                                                          <  2:1685077/140‑1 (MQ=255)                    gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACCTCCGGCCGGGCTAGA‑CCTGAAAAAccgggc                                                                                                                          >  2:523132/1‑135 (MQ=255)                     cgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGcc                                                                                                                         >  1:1831016/1‑140 (MQ=255)                     cgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGcc                                                                                                                         <  1:98951/140‑1 (MQ=255)                       cTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCAt                                                                                                                       <  2:627914/140‑1 (MQ=255)                            aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGa                                                                                                                  <  1:320709/140‑1 (MQ=255)                              tggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATg                                                                                                                <  2:404735/140‑1 (MQ=255)                              tggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATg                                                                                                                <  2:277949/140‑1 (MQ=255)                               ggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGa                                                                                                               <  1:1182815/140‑1 (MQ=255)                               ggtggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGa                                                                                                               <  1:1771079/140‑1 (MQ=255)                                 tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  2:307982/140‑1 (MQ=255)                                 tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  1:925679/140‑1 (MQ=255)                                   gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                           <  2:1137317/140‑1 (MQ=255)                                   gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                           <  2:375143/140‑1 (MQ=255)                                     gAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGta                                                                                                         >  1:1077461/1‑140 (MQ=255)                                      aGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGtaa                                                                                                        <  1:637993/140‑1 (MQ=255)                                       gCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGCGCCACGCCCGGCGGGGGTTGA‑ACTGGAAAAATGCGCCATGAAAATGAAGCGtaaa                                                                                                       >  2:245568/1‑139 (MQ=255)                                          gCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAc                                                                                                    >  2:950041/1‑140 (MQ=255)                                          gCAATCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCCTGAAGATGACGCGtaataaa                                                                                                    >  2:1626172/1‑139 (MQ=255)                                           cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             <  1:976732/130‑1 (MQ=255)                                           cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACg                                                                                                   <  2:1831016/140‑1 (MQ=255)                                           cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACg                                                                                                   >  1:1742201/1‑140 (MQ=255)                                           cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                             >  2:976732/1‑130 (MQ=255)                                             ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  1:1266353/140‑1 (MQ=255)                                             ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  2:1742201/140‑1 (MQ=255)                                             ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  2:1266580/140‑1 (MQ=255)                                                  gACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGt                                                                                            >  1:1551826/1‑140 (MQ=255)                                                          gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 <  2:1798133/127‑1 (MQ=255)                                                          gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCg                                                                                    >  1:411594/1‑140 (MQ=255)                                                          gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                                 >  1:1798133/1‑127 (MQ=255)                                                          gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCg                                                                                    >  1:1009417/1‑140 (MQ=255)                                                          gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCg                                                                                    >  1:1121773/1‑140 (MQ=255)                                                            ttCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAAATGCGCCCTGAAGAAGACGCGGAAAAAAGAAGAGGGTTCTAGCggg                                                                                  >  2:1572980/1‑140 (MQ=255)                                                                gTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGcgcg                                                                              >  1:859322/1‑140 (MQ=255)                                                                 tttGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAAAGTCCGGCGGGGTTAGG‑CCTGAAAAAATGCGCCATGAAGATGACGCGGAATAAAGAAGAGGGTTCTAGCGGGCGCgg                                                                             >  2:92508/1‑140 (MQ=255)                                                                 tttGTCGATACCGTGG‑‑GATAACGCCTCCTGT‑GGGTGTATGTCATCGGCGGTCCGGCGCAACGGCCGGCGGGGGTAGA‑ACCGGAAAACTGCGCCCTGAAGATGAAGCGCAATAAAGAAGAGGGTTCCAACGGGCGCGGCAg                                                                          >  2:232836/1‑140 (MQ=255)                                                                      cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  2:411594/140‑1 (MQ=255)                                                                      cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  2:1009417/140‑1 (MQ=255)                                                                      cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                        <  1:1626172/140‑1 (MQ=255)                                                                          gccGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATg                                                                    <  1:333731/139‑1 (MQ=255)                                                                              tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                                >  1:1582538/1‑140 (MQ=255)                                                                               ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                               <  1:523132/140‑1 (MQ=255)                                                                               ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATAAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                               >  2:68860/1‑140 (MQ=255)                                                                                  aGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGG‑CCTGGAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCCGGCGCGGCAGCGAAAGCCTGGGAc                                                            >  2:656846/1‑140 (MQ=255)                                                                                   gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                           >  1:285789/1‑140 (MQ=255)                                                                                    aTAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGACCGGCGGGGGTTGATCCTGAAAAA‑TTCGCCCAGAAGATAGAGCGGAATAAAGAAGAGGGTTTTTGCGGGCGCGGGCGCGAATGCCTGGGGCAt                                                          >  2:1409195/1‑140 (MQ=255)                                                                                      aaCGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGGAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCCGCAGCGAATGCCTGGGACATcc                                                        >  2:333731/1‑140 (MQ=255)                                                                                         gCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCt                                                     <  2:1077461/140‑1 (MQ=255)                                                                                             cctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAAt                                                 >  1:1802499/1‑140 (MQ=255)                                                                                             cctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTTTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTaaa                                                 >  2:1104950/1‑139 (MQ=255)                                                                                              ctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATg                                                <  2:1582538/140‑1 (MQ=255)                                                                                              ctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATg                                                >  1:1243837/1‑140 (MQ=255)                                                                                                        tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  2:235819/1‑140 (MQ=255)                                                                                                        tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTGATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  2:591926/1‑140 (MQ=255)                                                                                                        tATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                      >  1:1550446/1‑140 (MQ=255)                                                                                                             cATCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAAGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGCctgggcgg                                 >  2:146931/1‑136 (MQ=255)                                                                                                              aTCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGGAAAAATTCGCCCTGAAGATGAAGCGTAATAACGAAGAGGGTTCTAGTGGGCGCGGCAGAGAATGGCTGGGGCATCCGCTTAATGCGGCCGTCtggctggc                                >  2:370218/1‑140 (MQ=255)                                                                                                               tCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                               <  1:1572980/140‑1 (MQ=255)                                                                                                               tCGGCGGTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                               >  2:955381/1‑140 (MQ=255)                                                                                                                cggcggTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              >  1:1050628/1‑140 (MQ=255)                                                                                                                cggcggTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                              >  1:1471859/1‑140 (MQ=255)                                                                                                                  gcggTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGc                            >  2:1070037/1‑140 (MQ=255)                                                                                                                    ggTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa                          <  2:859322/140‑1 (MQ=255)                                                                                                                    ggTCCGGCGCAACGTCCGGCGGGGGTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGGCTGGCTGGCACGCaa                          >  2:1561156/1‑140 (MQ=255)                                                                                                                          gcgcAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc                    <  1:370218/140‑1 (MQ=255)                                                                                                                          gcgcAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc                    <  2:960754/140‑1 (MQ=255)                                                                                                                          gcgcAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc                    <  1:950041/140‑1 (MQ=255)                                                                                                                             cAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAg                 >  2:1032687/1‑140 (MQ=255)                                                                                                                                cGTCCGGCGGGGCTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCt              >  2:93134/1‑140 (MQ=255)                                                                                                                                     ggcggGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTg         >  1:663008/1‑140 (MQ=255)                                                                                                                                        ggggTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAAc      <  1:1104950/140‑1 (MQ=255)                                                                                                                                          ggTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCg    <  1:235819/140‑1 (MQ=255)                                                                                                                                          ggTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCg    >  2:584547/1‑140 (MQ=255)                                                                                                                                            ttAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  >  2:1254381/1‑140 (MQ=255)                                                                                                                                            ttAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCt  <  1:1032687/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGA‑CCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAACCGCT  >  NC_000913/372501‑372779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑