Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,409	C→A	P340Q (CCG→CAG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,409	0	C	A	100.0%	282.4 / NA	80	P340Q (CCG→CAG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/39);  total (41/39)

ACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCG  >  NC_000913/23273‑23545                                                                                                                                         |                                                                                                                                         aCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGga                                                                                                                                       >  2:691008/1‑140 (MQ=255) aCGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGga                                                                                                                                       <  2:1114261/140‑1 (MQ=255)  cGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGgac                                                                                                                                      <  1:1045779/140‑1 (MQ=255)  cGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGgac                                                                                                                                      <  1:399531/140‑1 (MQ=255)  cGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGgac                                                                                                                                      <  2:1326020/140‑1 (MQ=255)  cGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGgac                                                                                                                                      <  2:416284/140‑1 (MQ=255)  cGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGgac                                                                                                                                      <  2:415744/140‑1 (MQ=255)           gTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTAtgtg                                                                                                                             <  1:1284954/140‑1 (MQ=255)             gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGAt                                                                                                                           <  1:722243/140‑1 (MQ=255)             gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGAt                                                                                                                           >  1:914321/1‑140 (MQ=255)              cGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACt                                                                                                                                  <  1:1789382/132‑1 (MQ=255)              cGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACt                                                                                                                                  >  2:1789382/1‑132 (MQ=255)              cGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATc                                                                                                                          >  1:1132486/1‑140 (MQ=255)               ggAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCg                                                                                                                         >  1:1555518/1‑140 (MQ=255)               ggAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCg                                                                                                                         >  1:503920/1‑140 (MQ=255)               ggAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCg                                                                                                                         >  2:189910/1‑140 (MQ=255)                 aGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGt                                                                                                                       <  2:1132486/140‑1 (MQ=255)                        gctgctGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAAt                                                                                                                <  1:1825723/140‑1 (MQ=255)                          tgctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATAc                                                                                                              <  1:1654285/140‑1 (MQ=255)                                  tttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                                      <  1:1529064/140‑1 (MQ=255)                                  tttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                                      <  1:42905/140‑1 (MQ=255)                                  tttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                                      <  1:691008/140‑1 (MQ=255)                                   ttACCCTGCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCGACAGCCAGGACGACTATGTGATCGGTCAGAAATCCGGCCTGGaa                                                                                                     <  2:1064399/140‑1 (MQ=255)                                   ttACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaa                                                                                                     >  1:734166/1‑140 (MQ=255)                                      cccATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAAcc                                                                                                  >  1:597218/1‑140 (MQ=255)                                          tCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTa                                                                                              <  1:1033414/140‑1 (MQ=255)                                           ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa                                                                                             <  1:1834555/140‑1 (MQ=255)                                           ccGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTaa                                                                                             <  2:597218/140‑1 (MQ=255)                                            cGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAc                                                                                            >  1:500682/1‑140 (MQ=255)                                              tttATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAccc                                                                                          >  1:1295882/1‑140 (MQ=255)                                                tATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg                                                                                        <  1:102345/140‑1 (MQ=255)                                                 aTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                                                                                       >  1:1261697/1‑140 (MQ=255)                                                 aTGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                                                                                       >  1:1368503/1‑140 (MQ=255)                                                     gCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGc                                                                                   <  1:1178819/140‑1 (MQ=255)                                                        tCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                                <  2:602016/140‑1 (MQ=255)                                                        tCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                                >  2:976863/1‑140 (MQ=255)                                                        tCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                                >  1:1846169/1‑140 (MQ=255)                                                         cGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGc                                                                                                <  2:240322/123‑1 (MQ=255)                                                         cGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGc                                                                                                >  1:240322/1‑123 (MQ=255)                                                              ttCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGc                                                                          <  2:1846169/140‑1 (MQ=255)                                                                 cGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACt                                                                       >  1:1062022/1‑140 (MQ=255)                                                                       tCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc                                                                                  >  2:1343854/1‑123 (MQ=255)                                                                       tCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGcc                                                                                  <  1:1343854/123‑1 (MQ=255)                                                                                      ttACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCg                                                  <  2:914321/140‑1 (MQ=255)                                                                                             ggATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTgg                                           >  2:448913/1‑140 (MQ=255)                                                                                              gATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGa                                          >  1:400544/1‑140 (MQ=255)                                                                                               aTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATc                                                    >  1:327725/1‑129 (MQ=255)                                                                                               aTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATc                                                    <  2:327725/129‑1 (MQ=255)                                                                                               aTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGAt                                         >  1:1603111/1‑140 (MQ=255)                                                                                               aTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGAt                                         >  2:1497016/1‑140 (MQ=255)                                                                                                 gCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATgg                                       >  2:1568349/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  2:697129/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  2:822911/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     <  1:976863/140‑1 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  2:88896/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  1:1187962/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  2:96946/1‑140 (MQ=255)                                                                                                   cGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                     >  2:1851292/1‑140 (MQ=255)                                                                                                     gTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg                                   <  1:71015/140‑1 (MQ=255)                                                                                                     gTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg                                   <  2:500682/140‑1 (MQ=255)                                                                                                     gTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTg                                   <  1:1520608/140‑1 (MQ=255)                                                                                                       aCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGaa                                 >  1:1559909/1‑140 (MQ=255)                                                                                                        ccGGTGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc                                >  2:564682/1‑140 (MQ=255)                                                                                                           gTGCCGTTCACACCGCGCCTGGCCGTGGCCAGG‑CG‑CGGTGTGAACGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCtt                           >  1:1136924/1‑140 (MQ=255)                                                                                                            tGCCGTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCt                            >  1:205772/1‑140 (MQ=255)                                                                                                                gTTCACACCGCGCCTGGCCACGGCCAGGATGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCaa                        >  1:1699850/1‑140 (MQ=255)                                                                                                                gTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCaa                        >  2:1967/1‑140 (MQ=255)                                                                                                                gTTCACACCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCaa                        >  1:1524475/1‑140 (MQ=255)                                                                                                                      acCGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGaa                  >  1:707681/1‑140 (MQ=255)                                                                                                                       ccGCGCCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAAc                 <  1:857340/140‑1 (MQ=255)                                                                                                                           gcCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACa             >  2:276312/1‑140 (MQ=255)                                                                                                                           gcCTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACa             >  1:6539/1‑140 (MQ=255)                                                                                                                            ccTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt            <  2:1368503/140‑1 (MQ=255)                                                                                                                            ccTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt            <  2:1261697/140‑1 (MQ=255)                                                                                                                            ccTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt            <  1:96946/140‑1 (MQ=255)                                                                                                                            ccTGGCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt            <  1:88896/140‑1 (MQ=255)                                                                                                                                gCCACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcgtc        >  1:1358003/1‑140 (MQ=255)                                                                                                                                 ccACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtcgtcg       <  2:843312/140‑1 (MQ=255)                                                                                                                                  cACGGCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATcgtcgt      <  2:407593/140‑1 (MQ=255)                                                                                                                                     ggCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTgc   <  1:1497016/140‑1 (MQ=255)                                                                                                                                      gCCAGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTgcg  <  1:448913/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCG  >  NC_000913/23273‑23545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑