Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,299	G→T	intergenic (+90/‑179)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,299	0	G	T	100.0%	319.0 / NA	90	intergenic (+90/‑179)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/46);  total (44/46)

TCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTT  >  NC_000913/4215166‑4215435                                                                                                                                      |                                                                                                                                         tCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAAt                                                                                                                                    <  2:828480/140‑1 (MQ=255) tCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAAt                                                                                                                                    <  2:1688234/140‑1 (MQ=255)  cACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATa                                                                                                                                   >  2:443661/1‑140 (MQ=255)    cGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATaaa                                                                                                                                 >  1:1474941/1‑140 (MQ=255)     gCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAAt                                                                                                                                >  2:1311871/1‑140 (MQ=255)       cATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAAttt                                                                                                                              <  2:1487881/140‑1 (MQ=255)          aCGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTAt                                                                                                                           >  1:18129/1‑140 (MQ=255)          aCGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTAt                                                                                                                           >  1:1400746/1‑140 (MQ=255)           cGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTAtt                                                                                                                          <  2:1453244/140‑1 (MQ=255)           cGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTAtt                                                                                                                          <  2:1401100/140‑1 (MQ=255)           cGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTAtt                                                                                                                          <  1:1311871/140‑1 (MQ=255)            gATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTc                                                                                                                         <  1:443661/140‑1 (MQ=255)               cTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCGCCTTACCGCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCata                                                                                                                      <  1:1568403/140‑1 (MQ=255)               cTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATATATTTTATTCata                                                                                                                      <  2:1449188/140‑1 (MQ=255)                 aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAAtttt                                                                                                                             <  2:1822317/131‑1 (MQ=255)                 aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAAtttt                                                                                                                             >  1:1822317/1‑131 (MQ=255)                 aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATAtt                                                                                                                    >  1:684673/1‑140 (MQ=255)                   ggCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGt                                                                                                                  >  1:1515574/1‑140 (MQ=255)                   ggCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGt                                                                                                                  >  2:1879314/1‑140 (MQ=255)                   ggCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGt                                                                                                                  >  2:571059/1‑140 (MQ=255)                     cacaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTa                                                                                                                >  2:1745794/1‑140 (MQ=255)                     cacaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTa                                                                                                                >  1:1182951/1‑140 (MQ=255)                        aTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCata                                                                                                                      <  2:679314/131‑1 (MQ=255)                        aTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCata                                                                                                                      >  1:679314/1‑131 (MQ=255)                        aTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATca                                                                                                             >  1:1340253/1‑140 (MQ=255)                        aTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATca                                                                                                             >  1:966326/1‑140 (MQ=255)                          gAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATcaac                                                                                                           <  2:1474941/140‑1 (MQ=255)                          gAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATcaac                                                                                                           <  1:832858/140‑1 (MQ=255)                          gAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATcaac                                                                                                           >  2:1392487/1‑140 (MQ=255)                          gAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATcaac                                                                                                           <  2:684673/140‑1 (MQ=255)                             tCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAg                                                                                                        <  1:1849781/140‑1 (MQ=255)                             tCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAg                                                                                                        <  1:5450/140‑1 (MQ=255)                             tCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAg                                                                                                        <  2:1340253/140‑1 (MQ=255)                             tCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAg                                                                                                        <  1:1446357/140‑1 (MQ=255)                                 aCGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTg                                                                                                                   <  2:147392/125‑1 (MQ=255)                                 aCGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTg                                                                                                                   >  1:147392/1‑125 (MQ=255)                                  cGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTg                                                                                                                   >  1:1068880/1‑124 (MQ=255)                                  cGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTg                                                                                                                   <  2:1068880/124‑1 (MQ=255)                                  cGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATc                                                                                                   >  1:1136457/1‑140 (MQ=255)                                                 ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTttaatta                                                                                    >  1:1665986/1‑140 (MQ=255)                                                 ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTttaatta                                                                                    >  1:1762331/1‑140 (MQ=255)                                                 ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTttaatta                                                                                    >  2:400669/1‑140 (MQ=255)                                                 ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTttaatta                                                                                    >  1:1567953/1‑140 (MQ=255)                                                  tgtgATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTttaattaa                                                                                   <  1:874915/140‑1 (MQ=255)                                                    tgATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTaaaa                                                                                 >  1:1769846/1‑140 (MQ=255)                                                      aTAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATg                                                                               >  1:1342312/1‑140 (MQ=255)                                                         gTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGaa                                                                            >  1:1080916/1‑140 (MQ=255)                                                            gATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTaaaa                                                                                 <  2:502965/132‑1 (MQ=255)                                                            gATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTaaaa                                                                                 >  1:502965/1‑132 (MQ=255)                                                            gATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAAtt                                                                         >  2:721209/1‑140 (MQ=255)                                                              tCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGt                                                                       >  1:1434944/1‑140 (MQ=255)                                                                       gATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGAttt                                                              <  2:1862396/140‑1 (MQ=255)                                                                        aTTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGAtttt                                                             >  2:236564/1‑140 (MQ=255)                                                                         ttCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTg                                                            <  2:1182951/140‑1 (MQ=255)                                                                          tCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGc                                                           >  1:1603811/1‑140 (MQ=255)                                                                            aGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCAt                                                         <  2:18129/140‑1 (MQ=255)                                                                            aGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCAt                                                         >  1:1418196/1‑140 (MQ=255)                                                                              cACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCAttt                                                       <  2:396662/140‑1 (MQ=255)                                                                                ccTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTa                                                     <  2:1567953/140‑1 (MQ=255)                                                                                 cTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTaa                                                    <  2:1080916/140‑1 (MQ=255)                                                                                   tACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAAt                                                  >  1:396996/1‑140 (MQ=255)                                                                                     ccTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGa                                                <  2:1136457/140‑1 (MQ=255)                                                                                     ccTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGa                                                <  2:1418196/140‑1 (MQ=255)                                                                                     ccTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGa                                                <  1:571059/140‑1 (MQ=255)                                                                                     ccTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGa                                                <  2:1434944/140‑1 (MQ=255)                                                                                      cTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               <  1:1879314/140‑1 (MQ=255)                                                                                      cTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               <  2:1603811/140‑1 (MQ=255)                                                                                       tCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                              <  2:966326/140‑1 (MQ=255)                                                                                       tCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                              <  1:721209/140‑1 (MQ=255)                                                                                        cAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagta                                             >  1:1683488/1‑140 (MQ=255)                                                                                        cAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               <  2:240584/138‑1 (MQ=255)                                                                                        cAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               >  1:240584/1‑138 (MQ=255)                                                                                          ggCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagtagt                                           <  2:1769846/140‑1 (MQ=255)                                                                                          ggCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagtagt                                           >  1:486217/1‑140 (MQ=255)                                                                                           gCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTc                                          >  2:1579799/1‑140 (MQ=255)                                                                                                   gggTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTtgt                                  <  2:417350/140‑1 (MQ=255)                                                                                                     gTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAATAAGTTATCAAGCATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATTAGTAGTCTTAGTTGTGc                                >  2:1098285/1‑140 (MQ=255)                                                                                                     gTGCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGc                                <  2:396996/140‑1 (MQ=255)                                                                                                        ccTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               <  1:1529769/122‑1 (MQ=255)                                                                                                        ccTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGag                                               >  2:1529769/1‑122 (MQ=255)                                                                                                         cTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGaa                            >  2:362308/1‑140 (MQ=255)                                                                                                            ttttATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGa                         >  1:898716/1‑140 (MQ=255)                                                                                                            ttttATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGa                         >  2:558895/1‑140 (MQ=255)                                                                                                               tATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGaaaa                      <  2:1342312/140‑1 (MQ=255)                                                                                                                  ttCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAagag                   <  2:582963/140‑1 (MQ=255)                                                                                                                  ttCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAagag                   <  1:236564/140‑1 (MQ=255)                                                                                                                    ccGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTGTCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGca                 <  2:1236063/140‑1 (MQ=255)                                                                                                                    ccGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGca                 >  2:1529751/1‑140 (MQ=255)                                                                                                                       aaaCGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACaa              >  2:1318918/1‑140 (MQ=255)                                                                                                                         aCGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACg            <  1:1318918/140‑1 (MQ=255)                                                                                                                              ccTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCt       >  2:679795/1‑140 (MQ=255)                                                                                                                                  agcatGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGt   >  1:872190/1‑140 (MQ=255)                                                                                                                                  agcatGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGt   <  2:1515574/140‑1 (MQ=255)                                                                                                                                   gcatGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGtt  <  2:1683488/140‑1 (MQ=255)                                                                                                                                   gcatGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGtt  <  1:1579799/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTT  >  NC_000913/4215166‑4215435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑