Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,657,020	G→T	R146R (CGC→CGA)	ynfC ←	UPF0257 family lipoprotein YnfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,657,020	0	G	T	100.0%	353.1 / NA	99	R146R (CGC→CGA)	ynfC	UPF0257 family lipoprotein YnfC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (52/47);  total (52/47)

CCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTC  >  NC_000913/1656885‑1657155                                                                                                                                        |                                                                                                                                        ccGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGat                                                                                                                                     <  2:705664/140‑1 (MQ=255) ccGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGat                                                                                                                                     <  1:860402/140‑1 (MQ=255)    ttGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATAtt                                                                                                                                  >  1:840621/1‑140 (MQ=255)    ttGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATAtt                                                                                                                                  <  2:544773/140‑1 (MQ=255)    ttGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATAtt                                                                                                                                  <  2:1841377/140‑1 (MQ=255)    ttGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATAtt                                                                                                                                  >  2:1687443/1‑140 (MQ=255)      gATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTcc                                                                                                                                <  2:1594151/140‑1 (MQ=255)       attattCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCa                                                                                                                               >  1:701756/1‑140 (MQ=255)              agtaaagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCAt                                                                                                                        >  2:1379386/1‑140 (MQ=255)                  aagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCa                                                                                                                         <  2:203138/135‑1 (MQ=255)                  aagtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCa                                                                                                                         >  1:203138/1‑135 (MQ=255)                   agtaaCCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCAtttgtt                                                                                                                   >  1:99835/1‑140 (MQ=255)                        ccGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              <  1:1379386/140‑1 (MQ=255)                         cGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             <  1:1417772/140‑1 (MQ=255)                         cGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             <  1:201416/140‑1 (MQ=255)                          gCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTgctgct                                                                                                            >  2:1717681/1‑140 (MQ=255)                          gCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTgctgct                                                                                                            >  2:1488452/1‑140 (MQ=255)                          gCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTgctgct                                                                                                            <  2:701756/140‑1 (MQ=255)                            tgtgTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             >  1:5792/1‑137 (MQ=255)                            tgtgTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             <  2:5792/137‑1 (MQ=255)                            tgtgTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              >  2:214657/1‑136 (MQ=255)                            tgtgTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              <  1:214657/136‑1 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTGCTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTgg                                                                                                          <  2:1057431/138‑1 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             >  1:1140693/1‑135 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctgc                                                                                                             <  2:1140693/135‑1 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              <  2:183231/134‑1 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              >  1:183231/1‑134 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              <  2:1267571/134‑1 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTgg                                                                                                          >  1:1057431/1‑138 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCt                                                                                                        >  2:896590/1‑140 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCt                                                                                                        >  2:1543529/1‑140 (MQ=255)                              tgtAATCTAATTTTTTGATCGGATCCGTTGATGGCGGGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              >  1:1267571/1‑134 (MQ=255)                               gtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              <  2:1391332/133‑1 (MQ=255)                               gtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTtgctg                                                                                                              >  1:1391332/1‑133 (MQ=255)                               gtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCtt                                                                                                       >  2:109716/1‑140 (MQ=255)                               gtAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCtt                                                                                                       >  1:638741/1‑140 (MQ=255)                                   tCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAAt                                                                                                   >  1:1785317/1‑140 (MQ=255)                                     tAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCa                                                                                                 <  1:1488452/140‑1 (MQ=255)                                     tAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCa                                                                                                 <  2:1538271/140‑1 (MQ=255)                                     tAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCa                                                                                                 <  2:638741/140‑1 (MQ=255)                                       aTTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACg                                                                                               <  2:759707/140‑1 (MQ=255)                                       aTTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACg                                                                                               <  1:1099382/140‑1 (MQ=255)                                       aTTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACg                                                                                               <  2:840621/140‑1 (MQ=255)                                        ttttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCAc                                                                                                <  2:220823/138‑1 (MQ=255)                                        ttttttGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCAc                                                                                                >  1:220823/1‑138 (MQ=255)                                             tGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGaa                                                                                             <  2:1686715/136‑1 (MQ=255)                                             tGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGaa                                                                                             >  1:1686715/1‑136 (MQ=255)                                                 cGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTat                                                                                     <  1:286276/140‑1 (MQ=255)                                                    aTCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTatcat                                                                                  >  1:280890/1‑140 (MQ=255)                                                              ggcgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCgg                                                                               >  1:140365/1‑133 (MQ=255)                                                              ggcgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCgg                                                                               <  2:140365/133‑1 (MQ=255)                                                              ggcgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCa                                                                        >  2:19209/1‑140 (MQ=255)                                                              ggcgtggcgCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCa                                                                        >  2:1704825/1‑140 (MQ=255)                                                                         gACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCgg                                                             <  1:1543529/140‑1 (MQ=255)                                                                           cAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCa                                                           <  1:896590/140‑1 (MQ=255)                                                                           cAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCa                                                           <  1:1551656/140‑1 (MQ=255)                                                                           cAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCa                                                           <  2:280890/140‑1 (MQ=255)                                                                              aTAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGaa                                                        <  1:1717681/140‑1 (MQ=255)                                                                                  tGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTa                                                    <  2:1614888/140‑1 (MQ=255)                                                                                    ttttGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAAt                                                  >  1:1588438/1‑140 (MQ=255)                                                                                    ttttGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAAt                                                  >  1:104312/1‑140 (MQ=255)                                                                                      ttGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTTCCAACTCACCCCGGCAGAAGGTAATTc                                                >  1:956316/1‑140 (MQ=255)                                                                                         tCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGc                                             >  2:1826267/1‑140 (MQ=255)                                                                                            ttACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAg                                          >  2:766229/1‑140 (MQ=255)                                                                                             tACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGc                                         <  1:766229/140‑1 (MQ=255)                                                                                                   tCGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAt                                   >  1:1869562/1‑140 (MQ=255)                                                                                                    cGTGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAtt                                  >  2:1686223/1‑140 (MQ=255)                                                                                                      tGGTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAtttt                                <  2:1869562/140‑1 (MQ=255)                                                                                                       ggTTTTACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTc                               >  1:1142192/1‑140 (MQ=255)                                                                                                           ttACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTg                           <  1:727965/140‑1 (MQ=255)                                                                                                            tACCCAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGt                          >  2:949361/1‑140 (MQ=255)                                                                                                                cAGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAAc                      <  2:1084467/140‑1 (MQ=255)                                                                                                                 aGCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACg                     >  2:1307830/1‑140 (MQ=255)                                                                                                                  gCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGt                    >  2:600941/1‑140 (MQ=255)                                                                                                                  gCGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGt                    >  1:784669/1‑140 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAtttt                                <  1:1067741/127‑1 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCAtttt                                >  2:1067741/1‑127 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTccc                             >  2:294142/1‑130 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTccc                             <  1:294142/130‑1 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   >  2:1569483/1‑140 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   <  1:600941/140‑1 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   <  1:109716/140‑1 (MQ=255)                                                                                                                   cGGATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   >  2:1842766/1‑140 (MQ=255)                                                                                                                     gATAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTAct                 <  1:949361/140‑1 (MQ=255)                                                                                                                       tAACCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTActct               <  1:1826267/140‑1 (MQ=255)                                                                                                                        aaCCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   >  1:750133/1‑135 (MQ=255)                                                                                                                        aaCCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   <  2:750133/135‑1 (MQ=255)                                                                                                                        aaCCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTActctc              >  1:661661/1‑140 (MQ=255)                                                                                                                        aaCCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTActctc              <  1:1686223/140‑1 (MQ=255)                                                                                                                         aCCCTGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTActctct             >  2:1056888/1‑140 (MQ=255)                                                                                                                             tGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTg                           <  2:1295777/122‑1 (MQ=255)                                                                                                                             tGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTg                           >  1:1295777/1‑122 (MQ=255)                                                                                                                             tGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTg                           >  1:1854011/1‑122 (MQ=255)                                                                                                                             tGATCATCATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTg                           <  2:1854011/122‑1 (MQ=255)                                                                                                                               atcatcATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCa       >  2:669500/1‑140 (MQ=255)                                                                                                                               atcatcATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCa       >  1:41730/1‑140 (MQ=255)                                                                                                                               atcatcATATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCa       >  2:921173/1‑140 (MQ=255)                                                                                                                                  atcatATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCg    >  1:1713932/1‑140 (MQ=255)                                                                                                                                    catATCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGtc  >  1:1796005/1‑140 (MQ=255)                                                                                                                                     atatCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTa                   <  2:557814/122‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCGTTGATTATTCAGTAAAGTAACCGCTGTGTAATCTAATTTTTTGATCGGATCCGTTGATGGCGTGGCGCTGACAGATAATGTTTTGTCGTTACTTTTCGTGGTTTTACCCAGCGGATAACCCTGATCATCATAGCGATATTCCATCTGCATTTGTTTGCTGCTGGCTTTAATCACGAAGCCATTATCATCGGTTTCCCAACTCACCCCGGCAGAAGGTAATTCTGCTAGCTGGCATTTTCCCTGTAAACGTACTCTCTTCTCCAGCGTC  >  NC_000913/1656885‑1657155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑