Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,082,978	C→T	R75W (CGG→TGG)	sbcB →	exodeoxyribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,082,978	0	C	T	100.0%	200.3 / NA	57	R75W (CGG→TGG)	sbcB	exodeoxyribonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

CACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT  >  NC_000913/2082841‑2083113                                                                                                                                          |                                                                                                                                        cacaGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATgg                                                                                                                                       >  2:398127/1‑140 (MQ=255)  acaGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATggg                                                                                                                                      <  2:1450042/140‑1 (MQ=255)     gTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGa                                                                                                                                   >  1:1233179/1‑140 (MQ=255)      ttCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGaa                                                                                                                                  >  1:1462354/1‑140 (MQ=255)                             cGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAACATGGGCGAAAGGAGAAAACGAAGCCGCGTTTg                                                                                                           <  2:504748/140‑1 (MQ=255)                              gAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgc                                                                                                          <  1:626508/140‑1 (MQ=255)                              gAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgc                                                                                                          <  2:1107324/140‑1 (MQ=255)                               aaTTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgcc                                                                                                         >  1:1423822/1‑140 (MQ=255)                                 ttCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgccgc                                                                                                       <  1:1435718/140‑1 (MQ=255)                                  tCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgccgc                                                                                                       <  2:795789/139‑1 (MQ=255)                                  tCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTgccgc                                                                                                       >  1:795789/1‑139 (MQ=255)                                      tGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTa                                                                                                  <  2:492851/140‑1 (MQ=255)                                           tCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCAc                                                                                             <  1:1489783/140‑1 (MQ=255)                                            cGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCTGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACt                                                                                            <  2:1423822/140‑1 (MQ=255)                                            cGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACt                                                                                            <  2:1144446/140‑1 (MQ=255)                                              gCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCg                                                                                          >  2:873906/1‑140 (MQ=255)                                                  aCCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCttt                                                                                      >  1:334709/1‑140 (MQ=255)                                                     cGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTta                                                                                   <  2:1462354/140‑1 (MQ=255)                                                       aaGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtacc                                                                                 <  1:118087/140‑1 (MQ=255)                                                        aGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtaccg                                                                                <  1:398127/140‑1 (MQ=255)                                                        aGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtaccg                                                                                >  1:962881/1‑140 (MQ=255)                                                        aGTCTTTGACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtaccg                                                                                <  2:1235236/140‑1 (MQ=255)                                                             tttACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTGTTCACTCGCTTTTtaccgtaccg                                                                           >  1:928096/1‑140 (MQ=255)                                                              ttACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtacc                                                                                 <  2:637628/133‑1 (MQ=255)                                                              ttACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtacc                                                                                 >  1:637628/1‑133 (MQ=255)                                                                    cAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCt                                                                    >  1:426378/1‑140 (MQ=255)                                                                      aGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGt                                                                  >  1:617829/1‑140 (MQ=255)                                                                          cGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTc                                                              <  2:334709/140‑1 (MQ=255)                                                                          cGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTc                                                              <  2:752267/140‑1 (MQ=255)                                                                            cTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTg                                                            <  1:760100/140‑1 (MQ=255)                                                                            cTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTg                                                            >  2:760100/1‑140 (MQ=255)                                                                                  aCTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTac                                                      <  2:617829/140‑1 (MQ=255)                                                                                       ttACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTGTTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTAcaacaa                                                 <  2:928096/140‑1 (MQ=255)                                                                                       ttACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTAcaacaa                                                 >  1:1381838/1‑140 (MQ=255)                                                                                           cccAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgtg                                             >  1:540940/1‑140 (MQ=255)                                                                                              agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                          >  2:617560/1‑140 (MQ=255)                                                                                              agccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                          >  2:1097907/1‑140 (MQ=255)                                                                                                ccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                        >  2:812864/1‑140 (MQ=255)                                                                                                ccagGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                        >  1:834363/1‑140 (MQ=255)                                                                                                          tATTAATTACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGaa                              >  2:1308237/1‑140 (MQ=255)                                                                                                             taattaCCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                           <  1:812864/140‑1 (MQ=255)                                                                                                                  aCCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                      <  1:1097907/140‑1 (MQ=255)                                                                                                                  aCCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                      <  1:1308237/140‑1 (MQ=255)                                                                                                                  aCCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACg                      >  2:1500412/1‑140 (MQ=255)                                                                                                                   ccGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGc                     <  2:788902/140‑1 (MQ=255)                                                                                                                    cGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCa                    >  1:255222/1‑140 (MQ=255)                                                                                                                    cGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCT‑GGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                   >  2:757856/1‑140 (MQ=255)                                                                                                                      gTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                  <  1:1090685/140‑1 (MQ=255)                                                                                                                                 cAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt           >  2:1394963/1‑136 (MQ=255)                                                                                                                                 cAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt           <  1:1394963/136‑1 (MQ=255)                                                                                                                                 cAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg       >  2:1377375/1‑140 (MQ=255)                                                                                                                                 cAGGAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg       >  2:145034/1‑140 (MQ=255)                                                                                                                                    gAAGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa    <  1:617560/140‑1 (MQ=255)                                                                                                                                      aGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa    <  1:75814/138‑1 (MQ=255)                                                                                                                                      aGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa    >  2:75814/1‑138 (MQ=255)                                                                                                                                      aGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt  >  1:773588/1‑140 (MQ=255)                                                                                                                                      aGCATGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt  >  2:1458279/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT  >  NC_000913/2082841‑2083113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑