breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT43.0% 78.6 / 60.8 86S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009134,111,7470GT33.3% 93.1 / 31.9 45N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,568,5040TG31.5% 208.7 / 21.7 112T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009134,111,7520TC31.2% 79.1 / 12.7 48K155E (AAA→GAA) yiiQDUF1454 domain‑containing protein YiiQ
*NC_0009132,326,4500TG29.6% 126.9 / 41.4 71G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_00091329,4960TA29.0% 136.8 / 35.3 69intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_000913501,5750AC28.9% 73.7 / 13.4 38V555G (GTG→GGG) ybaLputative transporter YbaL
*NC_000913501,5640AC28.2% 77.2 / 13.4 39*559E (TAA→GAA) ybaLputative transporter YbaL
*NC_0009132,001,9180AC27.7% 129.5 / 24.8 65intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009133,206,4350CT27.5% 85.6 / 23.5 51intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009132,628,6250AC26.9% 107.0 / 19.7 52V105G (GTC→GGC) pdeFcyclic di‑GMP phosphodiesterase PdeF
*NC_0009133,556,2070TC26.1% 84.5 / 13.0 46S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
*NC_0009131,825,0610GA25.6% 71.3 / 11.2 39intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
*NC_0009132,256,9320GT25.6% 223.2 / 59.2 86A135D (GCC→GAC) psuTputative pseudouridine transporter
*NC_000913646,7470TG25.5% 106.8 / 21.3 51T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
*NC_0009132,667,1260AC24.6% 133.4 / 14.2 65Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_000913650,8090TG24.1% 133.3 / 11.1 58T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_000913888,0140TG24.0% 101.4 / 11.1 50noncoding (44/81 nt)rybBsmall regulatory RNA RybB
*NC_0009132,140,9260AC23.9% 167.2 / 12.0 71G229G (GGT→GGGasmAputative assembly protein AsmA
*NC_0009134,287,7190TA23.3% 196.6 / 33.8 86intergenic (‑348/‑45)acs/nrfAacetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase

Marginal new junction evidence (lowest skew 10 of 35 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)24 (0.380) 9/190 4.0 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375160NA (NA)32 (0.350)
+T
14/274 4.3 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375059NA (NA)17 (0.190)
+T
11/274 5.0 NA noncoding (28/34 nt) other REP31c
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375246NA (NA)12 (0.150)
+GAACGCCTTATCCGGT
8/244 5.4 NA noncoding (13/34 nt) other REP31g
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 374922 =NA (NA)11 (0.160) 6/202 5.4 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 2518036 =85 (0.920)7 (0.110)
+41 bp
5/194 5.6 12.1% intergenic (‑204/+5) pdeA/alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
?NC_000913 2521180 = 60 (0.650)noncoding (8/76 nt) valY tRNA‑Val
* ? NC_000913 3231254 =NA (NA)5 (0.070) 5/224 6.2 NA noncoding (2/34 nt) other REP231a
?NC_000913 3234722 = NA (NA)noncoding (3/34 nt) other REP233b
* ? NC_000913 2518036 =85 (0.920)5 (0.080)
+41 bp
3/194 6.7 9.3% intergenic (‑204/+5) pdeA/alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
?NC_000913 2521058 = 54 (0.580)noncoding (8/76 nt) valX tRNA‑Val
* ? NC_000913 2518036 =85 (0.920)5 (0.080)
+41 bp
3/194 6.7 7.9% intergenic (‑204/+5) pdeA/alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
?NC_000913 2520938 = 81 (0.880)noncoding (8/76 nt) valU tRNA‑Val
* ? NC_000913 781075 =122 (1.320)5 (0.080)
+41 bp
3/194 6.7 6.4% noncoding (8/76 nt) valZ tRNA‑Val
?NC_000913 2518036 = 85 (0.920)intergenic (‑204/+5) pdeA/alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala