Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,474,432	C→A	S134* (TCA→TAA)	yfdQ →	CPS‑53 (KpLE1) prophage; protein YfdQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,474,432	0	C	A	100.0%	213.6 / NA	61	S134* (TCA→TAA)	yfdQ	CPS‑53 (KpLE1) prophage; protein YfdQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/23);  total (38/23)

TGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGA  >  NC_000913/2474298‑2474567                                                                                                                                       |                                                                                                                                        tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTgg                                                                                                                                    >  1:1051225/1‑140 (MQ=255) tGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTgg                                                                                                                                    >  2:1244684/1‑140 (MQ=255)     gTCATTGTTTTTACCCTGGGTATTATTGAAAACCCGGGCCACGCAGAAAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGa                                                                                                                                <  2:1279950/140‑1 (MQ=255)            tgCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGCCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGAtt                                                                                                                         >  2:550219/1‑140 (MQ=255)                       gttattATTGATAAACCAGGCCACGAAAAAAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGc                                                                                                              <  2:1233080/135‑1 (MQ=255)                       gttACTATTAATGACCCAGGCCACGAAAAAACCACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGc                                                                                                              <  1:657154/138‑1 (MQ=255)                       ggTATTATTAAAAAACCGGGCCACGAAAAAAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGc                                                                                                              <  2:1015203/140‑1 (MQ=255)                       ggTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGc                                                                                                              >  2:436135/1‑140 (MQ=255)                        gTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGcc                                                                                                             >  2:1065431/1‑140 (MQ=255)                            tATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACt                                                                                                         >  1:260226/1‑140 (MQ=255)                            tATTGAAAACCCAGGCCACAAAAAAAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACt                                                                                                         <  2:298450/140‑1 (MQ=255)                              ttGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTAc                                                                                                       >  2:520039/1‑140 (MQ=255)                              ttGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTAc                                                                                                       >  1:889683/1‑140 (MQ=255)                                 atgaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCtt                                                                                                    >  1:851825/1‑140 (MQ=255)                                  tgaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTtg                                                                                                   >  1:1310082/1‑140 (MQ=255)                                  tgaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTtg                                                                                                   >  1:298450/1‑140 (MQ=255)                                  tgaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTtg                                                                                                   >  1:102643/1‑140 (MQ=255)                                   gaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTtgt                                                                                                  >  2:970639/1‑140 (MQ=255)                                   gaACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTtgt                                                                                                  >  2:676591/1‑140 (MQ=255)                                      ccAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTggg                                                                                               >  1:1271146/1‑140 (MQ=255)                                      ccAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTggg                                                                                               >  1:959322/1‑140 (MQ=255)                                        aGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCt                                                                                             >  2:1366780/1‑140 (MQ=255)                                          gccACGAAAATACCCCCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCttt                                                                                           <  2:102643/138‑1 (MQ=255)                                          gTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCttt                                                                                           <  1:520039/140‑1 (MQ=255)                                               gCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGc                                                                                      >  2:2770/1‑140 (MQ=255)                                               gCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGc                                                                                      >  2:805671/1‑140 (MQ=255)                                                cAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCt                                                                                     <  2:889683/140‑1 (MQ=255)                                                     aaCACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATgg                                                                                <  1:1065431/140‑1 (MQ=255)                                                            ccACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTg                                                                              <  1:1054382/135‑1 (MQ=255)                                                            ccACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTg                                                                              >  2:1054382/1‑135 (MQ=255)                                                             cACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCa                                                                        >  1:113/1‑140 (MQ=255)                                                             cACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCACTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCa                                                                        <  2:566142/140‑1 (MQ=255)                                                               ctctCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCAtt                                                                      <  1:436135/140‑1 (MQ=255)                                                               ctctCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCAtt                                                                      <  1:1244684/140‑1 (MQ=255)                                                                 ctcAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCa                                                                    <  1:9641/140‑1 (MQ=255)                                                                     aaCTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGca                                                                <  2:1154751/140‑1 (MQ=255)                                                                                aCAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGc                                                     <  2:959322/140‑1 (MQ=255)                                                                                    cACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACATAAGCgg                                                      >  2:159682/1‑135 (MQ=255)                                                                                    cACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACATAAGCgg                                                      <  1:159682/135‑1 (MQ=255)                                                                                    cACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCg                                                 >  1:164456/1‑140 (MQ=255)                                                                                    cACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCg                                                 >  1:1093237/1‑140 (MQ=255)                                                                                       cGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCa                                              <  1:550219/140‑1 (MQ=255)                                                                                              tGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTa                                       >  1:440010/1‑140 (MQ=255)                                                                                              tGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTa                                       >  2:1243470/1‑140 (MQ=255)                                                                                                   tgttgtCTGTTAACGGCGAGCGTAACTTCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATc                                  >  2:946718/1‑140 (MQ=255)                                                                                                   tgttgtCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATc                                  >  1:1181566/1‑140 (MQ=255)                                                                                                   tgttgtCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCCTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATc                                  >  2:1389873/1‑140 (MQ=255)                                                                                                     ttgtCTGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGTTTGAAGACCGGGCCGACCACCTTGTGGGCTTTGATGCTAATGGTTACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCAc                                >  2:1139717/1‑140 (MQ=255)                                                                                                          tGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTg                           >  2:38712/1‑140 (MQ=255)                                                                                                          tGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTg                           >  2:969066/1‑140 (MQ=255)                                                                                                          tGTTAACGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTg                           >  1:457233/1‑140 (MQ=255)                                                                                                               aCGGCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCa                      >  1:1242966/1‑140 (MQ=255)                                                                                                                  gCGAGCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAAc                   >  2:570621/1‑140 (MQ=255)                                                                                                                      gCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGAt                             >  2:306130/1‑126 (MQ=255)                                                                                                                      gCGTAACTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGAt                             <  1:306130/126‑1 (MQ=255)                                                                                                                          aaCTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGc           <  2:457233/140‑1 (MQ=255)                                                                                                                          aaCTCCCAGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGc           <  2:164456/140‑1 (MQ=255)                                                                                                                                 aGAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGAtttt    >  1:251689/1‑140 (MQ=255)                                                                                                                                  gAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTg   <  1:2770/140‑1 (MQ=255)                                                                                                                                  gAAATAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTg   <  2:161861/140‑1 (MQ=255)                                                                                                                                   aaaTAACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGa  >  2:781609/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCCGTCAGTGTGCTTAACCTGGGTACTATTGATGAACCAGGTCACGCAGATAACACCGCCACTCTCAAACTGAAAAAGACAGCACCGTTCTCTGCCCTGTTGTCTGTTAACGGCGAGCGTAACTCCCAGAAATCACTGGCAGAATGGATTGAAGACTGGGCCGACTACCTTGTGGGCTTTGATGCTAATGGTGACGCCATTCAGGCAACAAAAGCGGCTGCGGCAATCCGTAAAATCACGATTGAAGCAAACCAGACCGCTGATTTTGA  >  NC_000913/2474298‑2474567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑