Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,874,704	C→A	R239L (CGA→CTA)	cysN ←	sulfate adenylyltransferase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,874,704	0	C	A	100.0%	295.4 / NA	83	R239L (CGA→CTA)	cysN	sulfate adenylyltransferase subunit 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/40);  total (43/40)

CACCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAG  >  NC_000913/2874567‑2874840                                                                                                                                          |                                                                                                                                          cacCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGc                                                                                                                                         >  2:93378/1‑140 (MQ=255) cacCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGc                                                                                                                                         >  2:582223/1‑140 (MQ=255) cacCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGc                                                                                                                                         >  2:463663/1‑140 (MQ=255) cacCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGc                                                                                                                                         <  1:1331563/140‑1 (MQ=255)   ccAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTg                                                                                                                                       <  1:538581/140‑1 (MQ=255)   ccAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTg                                                                                                                                       <  1:776255/140‑1 (MQ=255)      gagGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAt                                                                                                                                    <  1:1395808/140‑1 (MQ=255)       agGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAtc                                                                                                                                   <  2:918285/140‑1 (MQ=255)       agGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAt                                                                                                                                    <  2:992535/139‑1 (MQ=255)       agGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAt                                                                                                                                    >  1:992535/1‑139 (MQ=255)           cagcaCTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCa                                                                                                                               <  2:252937/140‑1 (MQ=255)            agcaCTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAc                                                                                                                              >  1:1107573/1‑140 (MQ=255)            agcaCTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAc                                                                                                                              >  1:218909/1‑140 (MQ=255)             gcaCTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑g                                                                                                                            <  1:110432/140‑1 (MQ=255)             gcaCTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑g                                                                                                                            <  1:463663/140‑1 (MQ=255)                   tGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTcc                                                                                                                      >  1:554330/1‑140 (MQ=255)                   tGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTcc                                                                                                                      >  1:548309/1‑140 (MQ=255)                   tGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTcc                                                                                                                      >  1:1072213/1‑140 (MQ=255)                   tGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTcc                                                                                                                      >  1:359627/1‑140 (MQ=255)                   tGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTcc                                                                                                                      >  1:1118452/1‑140 (MQ=255)                      cacGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGc                                                                                                                   <  2:897969/140‑1 (MQ=255)                      cacGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGc                                                                                                                   <  1:367636/140‑1 (MQ=255)                          ttGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCa                                                                                                                               <  2:744144/125‑1 (MQ=255)                          ttGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCa                                                                                                                               >  1:744144/1‑125 (MQ=255)                          ttGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACtt                                                                                                               >  1:962612/1‑140 (MQ=255)                          ttGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACtt                                                                                                               >  1:455715/1‑140 (MQ=255)                           tGCCCTACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGATTCCAGCACTTc                                                                                                              >  1:599446/1‑140 (MQ=255)                           tGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACt                                                                                                                <  1:1004721/138‑1 (MQ=255)                           tGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACt                                                                                                                >  2:1004721/1‑138 (MQ=255)                           tGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTc                                                                                                              >  1:875495/1‑140 (MQ=255)                             cccGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATCGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGa                                                                                                            >  1:951096/1‑140 (MQ=255)                              ccGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAg                                                                                                           <  2:557304/140‑1 (MQ=255)                                gACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCa                                                                                                         <  1:930341/140‑1 (MQ=255)                                 aCTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAg                                                                                                        >  1:1322936/1‑140 (MQ=255)                                   ttCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCACATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAgtg                                                                                                      >  2:275005/1‑140 (MQ=255)                                      cACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCg                                                                                                   <  2:1072213/140‑1 (MQ=255)                                         gcgACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGAc                                                                                                <  2:1118452/140‑1 (MQ=255)                                         gcgACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGAc                                                                                                <  2:1069874/140‑1 (MQ=255)                                         gcgACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGAc                                                                                                <  2:548309/140‑1 (MQ=255)                                            aCCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGTGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCgg                                                                                                  >  1:308144/1‑135 (MQ=255)                                            aCCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGTGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCgg                                                                                                  <  2:308144/135‑1 (MQ=255)                                            aCCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGc                                                                                             >  2:292590/1‑140 (MQ=255)                                            aCCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGc                                                                                             >  1:1187776/1‑140 (MQ=255)                                                 aTGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTAc                                                                                        <  1:582223/140‑1 (MQ=255)                                                   gCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCa                                                                                      <  2:455715/140‑1 (MQ=255)                                                     cAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACg                                                                                    >  1:786650/1‑140 (MQ=255)                                                      aGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACgg                                                                                   <  2:359627/140‑1 (MQ=255)                                                      aGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACgg                                                                                   <  2:1322936/140‑1 (MQ=255)                                                                     ccACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCGCGGCATACTTTCACTTTg                                                                    <  2:599446/140‑1 (MQ=255)                                                                          aaaaTCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATg                                                               >  2:429873/1‑140 (MQ=255)                                                                           aaaTCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGc                                                              <  2:195044/140‑1 (MQ=255)                                                                                 agaTTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGCCCGCTGTACCACGGCATCCTTTCA‑TTTGCGATGCCACGTTg                                                       <  2:875495/140‑1 (MQ=255)                                                                                  gaTTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTg                                                       <  1:292590/140‑1 (MQ=255)                                                                                     tCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCg                                                    <  2:833633/140‑1 (MQ=255)                                                                                       gggCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGcc                                                  >  2:980031/1‑140 (MQ=255)                                                                                          cGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTATCGCCTTc                                               <  2:218909/140‑1 (MQ=255)                                                                                               aaCGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACAGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTg                                          <  2:554330/140‑1 (MQ=255)                                                                                                       gCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCa                                        >  1:1241325/1‑134 (MQ=255)                                                                                                       gCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCa                                        <  2:1241325/134‑1 (MQ=255)                                                                                                       gCACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGc                                  >  1:665957/1‑140 (MQ=255)                                                                                                        cACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCg                                 >  1:888182/1‑140 (MQ=255)                                                                                                        cACCGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAACTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCg                                 >  1:1293074/1‑140 (MQ=255)                                                                                                          ccGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTc                                               >  1:797649/1‑124 (MQ=255)                                                                                                          ccGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTc                                               <  2:797649/124‑1 (MQ=255)                                                                                                           cGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCACGGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGc                               >  2:444566/1‑140 (MQ=255)                                                                                                           cGGGAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGca                              >  1:731370/1‑140 (MQ=255)                                                                                                            gggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcac                             >  2:206807/1‑140 (MQ=255)                                                                                                            gggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcac                             >  2:1044483/1‑140 (MQ=255)                                                                                                             ggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcaca                            >  2:494680/1‑140 (MQ=255)                                                                                                             ggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcaca                            <  2:446227/140‑1 (MQ=255)                                                                                                             ggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcaca                            >  2:1048939/1‑140 (MQ=255)                                                                                                             ggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcaca                            >  2:73889/1‑140 (MQ=255)                                                                                                             ggAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcaca                            >  2:318732/1‑140 (MQ=255)                                                                                                              gAAGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTCTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACaa                           <  2:1187776/140‑1 (MQ=255)                                                                                                              gAAGCGCATTGGCTGAGCATCCACCACTAGCTGGAACTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCAGCACaa                           <  2:1293074/140‑1 (MQ=255)                                                                                                                aGCGCATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAg                         >  1:421066/1‑140 (MQ=255)                                                                                                                  cgcATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCg                       >  2:702547/1‑140 (MQ=255)                                                                                                                  cgcATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCg                       >  2:351322/1‑140 (MQ=255)                                                                                                                  cgcATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCg                       <  2:888182/140‑1 (MQ=255)                                                                                                                  cacATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCg                       <  1:275005/138‑1 (MQ=255)                                                                                                                    cATTGGCTGAGCATCCACCACTAGCTGGATCTCCACGGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCgg                      <  1:444566/140‑1 (MQ=255)                                                                                                                    cATTGGCTGAGCATCCACCACTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGa                     >  2:47626/1‑140 (MQ=255)                                                                                                                                       acTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAg  <  1:1293021/140‑1 (MQ=255)                                                                                                                                       acTAGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAg  <  1:351322/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          CACCAGAGGGCAGCACTTTGACACGTTGCCCGACTTCCACGCGACCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCAC‑GGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAG  >  NC_000913/2874567‑2874840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑