| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,222,207 | G→T | G248C (GGC→TGC) | ebgR → | DNA‑binding transcriptional repressor EbgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,222,207 | 0 | G | T | 98.4% | 213.7 / ‑3.2 | 62 | G248C (GGC→TGC) | ebgR | DNA‑binding transcriptional repressor EbgR |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (33/28); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGG > NC_000913/3222070‑3222343 | tGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGc > 1:552516/1‑140 (MQ=255) aaGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTg < 1:685369/140‑1 (MQ=255) gTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCg > 1:772361/1‑140 (MQ=255) gTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGc < 2:1086096/140‑1 (MQ=255) cgAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTc > 2:529866/1‑140 (MQ=255) gAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCa < 1:721746/140‑1 (MQ=255) ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAAc < 1:529866/140‑1 (MQ=255) gATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACg > 1:682649/1‑140 (MQ=255) gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGTAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGa > 2:787534/1‑140 (MQ=255) gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGa > 1:924928/1‑140 (MQ=255) gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGa < 2:772361/140‑1 (MQ=255) gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGa > 2:414763/1‑140 (MQ=255) cgcgGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGTAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGaa < 1:787534/140‑1 (MQ=255) cgcgGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGaa > 1:894725/1‑140 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACg < 2:1362025/129‑1 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACg > 1:1362025/1‑129 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa < 1:102378/140‑1 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa < 1:1231700/140‑1 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa < 2:552516/140‑1 (MQ=255) cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa < 1:217888/140‑1 (MQ=255) gcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATc < 1:957951/140‑1 (MQ=255) ggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATccc < 1:1404237/140‑1 (MQ=255) gTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCca < 1:630696/140‑1 (MQ=255) cAGTGCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCCATCGGCGTACTGAGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGata < 2:1229707/140‑1 (MQ=255) cgtcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCg < 1:471528/140‑1 (MQ=255) cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCtt > 1:204576/1‑140 (MQ=255) tGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCg > 2:564113/1‑140 (MQ=255) tGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCg < 2:991233/140‑1 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTa < 1:346827/140‑1 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc < 2:204576/140‑1 (MQ=255) tGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc < 1:564113/140‑1 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTa > 2:105745/1‑137 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTa < 1:105745/137‑1 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACg > 2:543624/1‑140 (MQ=255) gCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa > 2:322520/1‑140 (MQ=255) gCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa > 2:802245/1‑140 (MQ=255) tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc > 2:633113/1‑140 (MQ=255) tGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc > 1:989031/1‑140 (MQ=255) cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGa > 1:827926/1‑140 (MQ=255) cgcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGa > 2:1199316/1‑140 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa > 2:839586/1‑123 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa < 1:839586/123‑1 (MQ=255) gcgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAt > 1:637695/1‑140 (MQ=255) ggAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTa > 2:419698/1‑140 (MQ=255) aTCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg < 1:271239/140‑1 (MQ=255) aTCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccg < 2:924928/140‑1 (MQ=255) cGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTccgccg < 1:419698/140‑1 (MQ=255) ggCACTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGctct < 2:682649/140‑1 (MQ=255) aCTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCa < 1:1199316/140‑1 (MQ=255) cTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAc > 1:330621/1‑140 (MQ=255) cTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAc > 1:1194435/1‑140 (MQ=255) cTGTTTGTTGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCAc > 2:1144496/1‑140 (MQ=255) gtttgttGCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCg > 1:586537/1‑140 (MQ=255) tgCTTCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCa > 2:140784/1‑140 (MQ=255) ttCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATcc < 2:330621/140‑1 (MQ=255) ttCCGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATcc > 1:71633/1‑140 (MQ=255) ccGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCAt > 1:349731/1‑140 (MQ=255) ccGATTCCATTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCAt > 1:1210873/1‑140 (MQ=255) aTTGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAgat > 2:953021/1‑140 (MQ=255) tGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAgatga > 1:412202/1‑140 (MQ=255) tGCTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAgatga > 2:1059775/1‑140 (MQ=255) cTATCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGatgatg > 1:55671/1‑140 (MQ=255) aTCTGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATggg < 1:1059775/140‑1 (MQ=255) | TGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGG > NC_000913/3222070‑3222343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |