Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,476,668	G→T	A251E (GCG→GAG)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,476,668	0	G	T	100.0%	208.6 / NA	59	A251E (GCG→GAG)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/29);  total (30/29)

TTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCA  >  NC_000913/3476531‑3476803                                                                                                                                          |                                                                                                                                        ttCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGctct                                                                                                                                       >  1:1003044/1‑140 (MQ=255)  tCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTg                                                                                                                                      <  2:1376342/140‑1 (MQ=255)                     tataTCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGctc                                                                                                                   <  1:410372/140‑1 (MQ=255)                        atCTGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTcaca                                                                                                                              >  2:23674/1‑126 (MQ=255)                        atCTGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTcaca                                                                                                                              <  1:23674/126‑1 (MQ=255)                        atCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTAc                                                                                                                >  1:292442/1‑140 (MQ=255)                             gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCcagcag                                                                                                                      <  1:964894/129‑1 (MQ=255)                             gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCcagcag                                                                                                                      >  2:964894/1‑129 (MQ=255)                             gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCaa                                                                                                           >  1:1271469/1‑140 (MQ=255)                             gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCaa                                                                                                           >  1:976030/1‑140 (MQ=255)                             gCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCGGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCaa                                                                                                           >  2:181735/1‑140 (MQ=255)                                tGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAaca                                                                                                        <  2:1271469/140‑1 (MQ=255)                                 gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                                                                                       <  2:976030/140‑1 (MQ=255)                                 gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                                                                                       >  2:832522/1‑140 (MQ=255)                                 gTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAacac                                                                                                       <  1:1030578/140‑1 (MQ=255)                                  taataaAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTATACGTCAAACAcc                                                                                                      <  1:424317/140‑1 (MQ=255)                                      aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAggg                                                                                                  <  2:318011/140‑1 (MQ=255)                                      aaaaaaaCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCGGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAggg                                                                                                  <  1:181735/140‑1 (MQ=255)                                            aCCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAAt                                                                                            >  2:165258/1‑140 (MQ=255)                                               gccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCg                                                                                         >  2:438580/1‑140 (MQ=255)                                               gccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCg                                                                                         <  2:120100/140‑1 (MQ=255)                                                ccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTc                                                                                          <  1:1170369/138‑1 (MQ=255)                                                ccgccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTc                                                                                          >  2:1170369/1‑138 (MQ=255)                                                   ccTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTgg                                                                                     <  1:165258/140‑1 (MQ=255)                                                    cTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTggg                                                                                    <  2:736596/140‑1 (MQ=255)                                                          aGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATcccc                                                                              <  1:988808/140‑1 (MQ=255)                                                          aGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATcccc                                                                              <  1:691367/140‑1 (MQ=255)                                                              ggcggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGaa                                                                          >  1:1172899/1‑140 (MQ=255)                                                              ggcggTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCCTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGaa                                                                          >  1:834300/1‑140 (MQ=255)                                                                       tAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCtt                                                                 <  1:765369/140‑1 (MQ=255)                                                                            cTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCg                                                            <  2:562952/140‑1 (MQ=255)                                                                               aTTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                         >  2:686965/1‑140 (MQ=255)                                                                               aTTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                         >  2:608638/1‑140 (MQ=255)                                                                               aTTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTaa                                                         >  1:294033/1‑140 (MQ=255)                                                                                 tttttAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGc                                                       <  1:608638/140‑1 (MQ=255)                                                                                  ttttAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGcc                                                      <  2:292442/140‑1 (MQ=255)                                                                                     tAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGt                                                   >  1:1007586/1‑140 (MQ=255)                                                                                      aGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGtt                                                  >  2:145310/1‑140 (MQ=255)                                                                                         aGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCg                                                            <  1:952930/127‑1 (MQ=255)                                                                                         aGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCg                                                            >  2:952930/1‑127 (MQ=255)                                                                                           aaTCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCtggt                                             >  2:512263/1‑140 (MQ=255)                                                                                           aaTCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCtggt                                             >  1:1090767/1‑140 (MQ=255)                                                                                             tCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGc                                                       >  1:1184304/1‑128 (MQ=255)                                                                                             tCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGc                                                       <  2:1184304/128‑1 (MQ=255)                                                                                                  ggCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATaa                                      <  1:438580/140‑1 (MQ=255)                                                                                                  ggCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATaa                                      <  1:512263/140‑1 (MQ=255)                                                                                                  ggCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATaa                                      <  2:1172899/140‑1 (MQ=255)                                                                                                  ggCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATaa                                      <  1:686965/140‑1 (MQ=255)                                                                                                       tCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGt                                 >  1:332305/1‑140 (MQ=255)                                                                                                       tCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGt                                 >  2:1287950/1‑140 (MQ=255)                                                                                                        cGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGtt                                >  1:402983/1‑140 (MQ=255)                                                                                                                       cTTTGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCtt                 >  1:1059400/1‑140 (MQ=255)                                                                                                                          tGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCt              >  1:876051/1‑140 (MQ=255)                                                                                                                          tGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCt              >  2:933799/1‑140 (MQ=255)                                                                                                                          tGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCt              >  2:779875/1‑140 (MQ=255)                                                                                                                          tGCATCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCt              >  2:1306280/1‑140 (MQ=255)                                                                                                                              tCAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGAt          <  2:1090767/140‑1 (MQ=255)                                                                                                                               cAGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATg         <  2:402983/140‑1 (MQ=255)                                                                                                                                aGCCTTCGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTCCCGCCTTTCTTGATGt        <  2:1059400/140‑1 (MQ=255)                                                                                                                                      cGGCTCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCa  >  1:276849/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TTCCGCTTTCCAGCACTAATTATATCCGGCCTGTAATAAAAAAACCGCCGCCTGGTCAGGCGGCGGTTCTTAATGCTTATTTTTTAGCAGAATCTGCGGCTTTCGCATCAGCTTCCGGCTTTGCATCAGCCTTCGGCGCTGGTTTCACATCCAGCAGCTCTACGTCAAACACCAGGGTAGAATTCGGTGGGATCCCCGGAACACCCGCTTTGCCGTAAGCCAGTTCTGGTGGAATAACCAGTTTGATCTTACCGCCTTTCTTGATGTTCTTCA  >  NC_000913/3476531‑3476803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑