Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,616,549 C→A S123* (TCG→TAG)  nikC → Ni(2(+)) ABC transporter membrane subunit NikC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,616,5490CA100.0% 129.0 / NA 39S123* (TCG→TAG) nikCNi(2(+)) ABC transporter membrane subunit NikC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/22);  total (17/22)

GGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCT  >  NC_000913/3616412‑3616681
                                                                                                                                         |                                                                                                                                    
ggtAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGt                                                                                                                                    >  1:715159/1‑140 (MQ=255)
       gcctgcctGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTAt                                                                                                                             <  1:1238728/140‑1 (MQ=255)
         ctgcctgcTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATgg                                                                                                                           <  1:892293/140‑1 (MQ=255)
         ctgcctgcTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATgg                                                                                                                           <  1:9401/140‑1 (MQ=255)
            cctgcTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTg                                                                                                                        >  2:253170/1‑140 (MQ=255)
                          tGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCAcc                                                                                                          >  1:54777/1‑140 (MQ=255)
                           gACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGGGCTGATCAGGCCACCCCGGGCGCCGCCGATATGTTTATTATCTTCCCGCCCCCGATTCTGTAGTTTTTTAAGGTTTGCGGGCTcctgaccc                                                                                                         >  2:959845/1‑133 (MQ=255)
                             cATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACTATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCggg                                                                                                       <  1:257441/140‑1 (MQ=255)
                               ttAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCt                                                                                                     <  1:959845/140‑1 (MQ=255)
                                  gggCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGAc                                                                                                  <  2:722167/140‑1 (MQ=255)
                                     cTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCaa                                                                                               <  1:612992/140‑1 (MQ=255)
                                      tGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCCGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGGTTCTGTAGTTCTTTATGGTTGGCGGGCTCGGCACCGGGCTGACCAAc                                                                                              >  2:51749/1‑140 (MQ=255)
                                      tGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAAc                                                                                              >  1:1370159/1‑140 (MQ=255)
                                        gTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                                                            >  1:460672/1‑140 (MQ=255)
                                        gTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                                                            >  2:1322685/1‑140 (MQ=255)
                                        gTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGGCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGt                                                                                            >  2:189637/1‑140 (MQ=255)
                                              ggcggcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTat                                                                                      >  1:892275/1‑140 (MQ=255)
                                                  gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTTTGACCTTCCCCACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                                                                  >  2:1062295/1‑140 (MQ=255)
                                                  gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                                                                  >  2:372985/1‑140 (MQ=255)
                                                  gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                                                                  >  2:614078/1‑140 (MQ=255)
                                                  gcAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGAATCTGTAGGTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgcc                                                                                  >  1:1305308/1‑140 (MQ=255)
                                                   cAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTTTTGTTGGCGTGCTCGGCACCGGGCCGACCAACGTAATTatcgcca                                                                                 >  2:85160/1‑140 (MQ=255)
                                                      cgcCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGAATCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcg                                                                              <  2:1305308/140‑1 (MQ=255)
                                                        ccGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                                                            <  2:504065/140‑1 (MQ=255)
                                                        ccGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTatcgccatcgcc                                                                            <  2:754738/140‑1 (MQ=255)
                                                                 ttGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCAc                                                                   <  2:1370159/140‑1 (MQ=255)
                                                                  ttgcgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACt                                                                  <  2:501984/138‑1 (MQ=255)
                                                                     cgggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTggg                                                               <  2:892275/140‑1 (MQ=255)
                                                                       ggcgCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCg                                                             <  2:1196949/140‑1 (MQ=255)
                                                                         cgcgTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                                                           <  1:380144/140‑1 (MQ=255)
                                                                                     gCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCAt                                               <  2:54777/140‑1 (MQ=255)
                                                                                          caTGCGCGTCGCCGATATGTTTGTGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                                                           >  1:1311730/1‑123 (MQ=255)
                                                                                          caTGCGCGTCGCCGATATGTTTGTGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTg                                                           <  2:1311730/123‑1 (MQ=255)
                                                                                          caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc                                                   <  2:737806/131‑1 (MQ=255)
                                                                                          caTGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCAc                                                   >  1:737806/1‑131 (MQ=255)
                                                                                            tGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTgcgc                                        >  2:879853/1‑140 (MQ=255)
                                                                                                       gATATGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGAt                             <  1:614078/140‑1 (MQ=255)
                                                                                                         tatGTTTATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGAttt                           <  1:351285/140‑1 (MQ=255)
                                                                                                             tttATGACCTTCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACt                       <  1:879853/140‑1 (MQ=255)
                                                                                                                       tCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGAt                             >  1:1373121/1‑124 (MQ=255)
                                                                                                                       tCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGAt                             <  2:1373121/124‑1 (MQ=255)
                                                                                                                       tCCCGACCTCGATTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAAcgc             >  2:398140/1‑140 (MQ=255)
                                                                                                                                  aTTCTGTAGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCt  >  1:561304/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                    
GGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGCTGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCCTGGTGATTTCACTACGCCAACGCGAGTTTGTGCT  >  NC_000913/3616412‑3616681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑