Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,628,625	0	A	C	21.7%	146.2 / 13.5	60	V105G (GTC→GGC)	pdeF	cyclic di‑GMP phosphodiesterase PdeF
Reads supporting (aligned to +/- strand):  ref base A (41/6);  new base C (0/13);  total (41/19)
Fisher's exact test for biased strand distribution p-value = 5.25e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAA  >  NC_000913/2628486‑2628764                                                                                                                                            |                                                                                                                                             gCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                                              <  1:1574002/140‑1 (MQ=255)  cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                             >  1:16199/1‑140 (MQ=255)  cTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAc                                                                                                                                             >  2:1834715/1‑140 (MQ=255)   tCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                                              >  1:1789447/1‑138 (MQ=255)   tCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                                              <  2:1789447/138‑1 (MQ=255)   tCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAcc                                                                                                                                            <  1:675275/140‑1 (MQ=255)   tCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAcc                                                                                                                                            <  2:1823778/140‑1 (MQ=255)    cGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGAccc                                                                                                                                           <  1:1073225/140‑1 (MQ=255)      ccAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTg                                                                                                                                         <  1:1834715/140‑1 (MQ=255)               cTACAAATGCAGAAAACGGAAAAAGTATCAAAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATCCGCTGGAAAATTAAACGGGTACCCCCATGTGAAACGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAA‑CCGccc                                                                                                                               <  2:1329661/140‑1 (MQ=255)                 aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCa                                                                                                                             >  1:1209010/1‑140 (MQ=255)                 aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCa                                                                                                                             >  1:512935/1‑140 (MQ=255)                  aaaaTGCAACAAACTGAAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCCGCCAAAATATGCGGTGGAAAATTAACCGGGTATCCCCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCaa                                                                                                                            <  2:16199/139‑1 (MQ=255)                    aaTGCAGCAAACTGAAAAAGTATCAAAAACAGCGTTGCAGGAAACAAAATCTGCCAGAATATACGCTGGAAAATTAAACGGGTATCCCCATGTGAAACGTTTTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACa                                                                                                                          <  2:609923/140‑1 (MQ=255)                       gcagcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACagag                                                                                                                       >  1:660686/1‑140 (MQ=255)                       gcagaaAACTGAAAAAGTATAAAAAACAGCGTGGAAGAAAACACAATCTGCCAGAATATACGCGGGAAAATAAAACGGTTATCCCCATGTAAAACGTTTTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACagag                                                                                                                       <  2:1962279/140‑1 (MQ=255)                        cagcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAgaga                                                                                                                      >  2:1570548/1‑140 (MQ=255)                           caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGaatg                                                                                                                   >  2:1837597/1‑140 (MQ=255)                           caAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGaatg                                                                                                                   >  1:609923/1‑140 (MQ=255)                                tgaaaaaTTATAAGAAACAGCGTTGCAGAAAACACAATCTGCAAAAATATCCGCTGGGAAATTAAACGGGTATCACCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGaatgataat                                                                                                              <  2:20950/137‑1 (MQ=255)                                tGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGaatgatctg                                                                                                              >  1:502152/1‑137 (MQ=255)                                tGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGaatgataat                                                                                                              >  1:1070131/1‑140 (MQ=255)                                      aGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATgataatgataat                                                                                                        <  1:153660/140‑1 (MQ=255)                                          tCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATaaaa                                                                                                    >  1:911365/1‑140 (MQ=255)                                          tCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATaaaa                                                                                                    >  1:1247703/1‑140 (MQ=255)                                            aaaaaCAGCGTTGCAGAAAACACAATCTGCAAAAAAATCCGGGGGAAAATAAAACGGGTATCCCCATGTAAAACGTTTTTCCGCGGGGGAAAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAAtt                                                                                                  <  2:1851744/138‑1 (MQ=255)                                            aGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAAtt                                                                                                  >  1:1647608/1‑140 (MQ=255)                                             gAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTa                                                                                                 >  1:1037611/1‑140 (MQ=255)                                                agggggTTGAAGAAAACAAAATCGGCAAAAATACACGTTGGAAAATAAAACGGGTACGACAATGAAAAATGTTTTTACGCGGGGGAGAAAAGCCCCTTTAC‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc                                                                                              <  2:135800/135‑1 (MQ=255)                                                    cGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTa                                                                                          >  1:1673505/1‑140 (MQ=255)                                                         gagaaaaCACAATCTGCCAAAATATACGCTGGAAAATAAAACGGGTATCCCCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt                                                                                     <  2:1070131/136‑1 (MQ=255)                                                              aaCACAATCTGCCAGAATATACGTTGGAAAATTAAACGGGTATCCCCATGTGAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGt                                                                                <  1:1837597/140‑1 (MQ=255)                                                              aaCACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGctg                                                                                >  1:135800/1‑138 (MQ=255)                                                                        gCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCTGTTAATGATAGCGTTTCGTaaaaa                                                                      >  1:1058060/1‑140 (MQ=255)                                                                        gCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTaaaaa                                                                      >  1:1508521/1‑140 (MQ=255)                                                                             aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                                 >  1:1405938/1‑140 (MQ=255)                                                                             aaTATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                                 <  2:1247703/140‑1 (MQ=255)                                                                             aaTATACGCTGGAAAATAAACCGGGTACCACCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCTGTTAATGATAGCGTTTCGTAAAAACCCAg                                                                 <  2:1058060/140‑1 (MQ=255)                                                                               tataCGCGGGAAAATAAAACGGTTACCCCCATGTAAAACGTTGTTCCGCGGGGAAAAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGct                                                               <  2:1037611/140‑1 (MQ=255)                                                                                    gCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCa                                                          >  1:1161410/1‑140 (MQ=255)                                                                                       gggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCatat                                                       >  1:794634/1‑140 (MQ=255)                                                                                        ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                      >  1:859278/1‑140 (MQ=255)                                                                                        ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                      >  1:1028509/1‑140 (MQ=255)                                                                                        ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                      >  1:642693/1‑140 (MQ=255)                                                                                        ggAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATAtt                                                      >  1:1793508/1‑140 (MQ=255)                                                                                             ttaagggggTACCACAATGTAAACCGTTGTTACGGGGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCgg                                                 <  2:897277/134‑1 (MQ=255)                                                                                                  ggggTATCACCATGTGAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaa                                            <  2:911365/139‑1 (MQ=255)                                                                                                   gggTATCACCATGTAAAACTTTTTTACGCGGGGGATAAAAGCCCTTGAAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaaaa                                           <  2:623846/140‑1 (MQ=255)                                                                                                     gTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAAt                                         >  1:1707368/1‑140 (MQ=255)                                                                                                       aTCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATcc                                       >  1:1064682/1‑140 (MQ=255)                                                                                                         ccccATGTGAATGTTTGTAACGCGGGGGAGAAAAGCCCTTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                     <  2:1405938/138‑1 (MQ=255)                                                                                                         ccataTGTAAAATGTTTTTCCGCTGGGGAAGAAACGCCTGATAT‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                     <  2:642693/136‑1 (MQ=255)                                                                                                         cACCATGTAAAACTTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                     <  2:1239222/140‑1 (MQ=255)                                                                                                         cACCATGTAAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                     <  2:859278/140‑1 (MQ=255)                                                                                                         cACCATGAAAACCTTTTTTACGCGGGGGAGAAAAGCCCTTTTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                                     <  2:1028509/140‑1 (MQ=255)                                                                                                             aTGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                                 >  2:54300/1‑140 (MQ=255)                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                                 <  2:713180/139‑1 (MQ=255)                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAgg                                 >  1:713180/1‑139 (MQ=255)                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                                >  2:1400786/1‑140 (MQ=255)                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                                >  2:699554/1‑140 (MQ=255)                                                                                                              tgtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc                                >  1:623846/1‑140 (MQ=255)                                                                                                               gtgAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                               <  2:1064682/140‑1 (MQ=255)                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                               >  2:246321/1‑140 (MQ=255)                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                               >  2:951406/1‑140 (MQ=255)                                                                                                               gtgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg                               >  1:1714255/1‑140 (MQ=255)                                                                                                                tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGa                              >  2:131618/1‑140 (MQ=255)                                                                                                                tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGa                              >  2:91205/1‑140 (MQ=255)                                                                                                                      gttgttACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTg                        >  1:907568/1‑140 (MQ=255)                                                                                                                        tgttACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGc                      >  1:1109601/1‑140 (MQ=255)                                                                                                                               ccGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgccg               >  2:933656/1‑140 (MQ=255)                                                                                                                                 ggggAG‑AAAGCCCCTTAAACCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATgccgcc             <  2:1517229/140‑1 (MQ=255)                                                                                                                                  gggAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGccc            >  1:486523/1‑140 (MQ=255)                                                                                                                                        aaaGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGa      <  2:1673505/140‑1 (MQ=255)                                                                                                                                         aaGCCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaa     <  1:1760571/140‑1 (MQ=255)                                                                                                                                            aCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa  >  2:260559/1‑140 (MQ=255)                                                                                                                                            aCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa  >  1:1517229/1‑140 (MQ=255)                                                                                                                                            aCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa  <  1:933656/140‑1 (MQ=255)                                                                                                                                            aCCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGaaaaa  >  1:719726/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                             GCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAA‑CCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAATGCCGCCCAGCTGAAAAA  >  NC_000913/2628486‑2628764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑