BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,567	C→A	Q285K (CAG→AAG)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,567	0	C	A	100.0%	516.1 / NA	143	Q285K (CAG→AAG)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (79/64); total (79/64)

Only 100 of 143 total aligned reads displayed.

CAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCC  >  NC_000913/19430‑19702

cAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAg                                                                                                                                       >  2:1228924/1‑140 (MQ=255)

 aaTCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGc                                                                                                                                      >  1:355665/1‑140 (MQ=255)

 aaTCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGc                                                                                                                                      >  1:1842894/1‑140 (MQ=255)

   tCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGa                                                                                                                                    <  2:436268/140‑1 (MQ=255)

   tCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGa                                                                                                                                    <  1:1228924/140‑1 (MQ=255)

   tCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGa                                                                                                                                    >  2:534472/1‑140 (MQ=255)

     ttCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAAt                                                                                                                                  <  1:431667/140‑1 (MQ=255)

     ttCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAAt                                                                                                                                  <  2:315442/140‑1 (MQ=255)

     ttCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAAt                                                                                                                                  >  2:729715/1‑140 (MQ=255)

        gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               <  2:1620475/140‑1 (MQ=255)

        gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               <  2:204695/140‑1 (MQ=255)

        gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               <  1:1968126/140‑1 (MQ=255)

        gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               >  2:1953450/1‑140 (MQ=255)

        gTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               >  1:1481939/1‑140 (MQ=255)

         ttGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGc                                                                                                                              >  2:1292364/1‑140 (MQ=255)

          tGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCa                                                                                                                             <  2:1677812/140‑1 (MQ=255)

          tGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCa                                                                                                                             <  1:534472/140‑1 (MQ=255)

          tGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCa                                                                                                                             <  2:1436743/140‑1 (MQ=255)

            ccccAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAAt                                                                                                                           <  2:1660942/140‑1 (MQ=255)

            ccccAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAAt                                                                                                                           <  1:816387/140‑1 (MQ=255)

                      ttATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGattatt                                                                                                                 >  1:575408/1‑140 (MQ=255)

                       tATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               >  2:1809210/1‑125 (MQ=255)

                       tATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTg                                                                                                                               <  1:1809210/125‑1 (MQ=255)

                         tGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTg                                                                                                              <  2:1288676/140‑1 (MQ=255)

                          gCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTgc                                                                                                             <  2:1774375/140‑1 (MQ=255)

                             tatGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCt                                                                                                          <  2:1842894/140‑1 (MQ=255)

                              atGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACgg                                                                                                                       <  1:1071460/126‑1 (MQ=255)

                              atGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCtt                                                                                                         >  1:1729460/1‑140 (MQ=255)

                              atGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCtt                                                                                                         >  1:628441/1‑140 (MQ=255)

                               tGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCttt                                                                                                        >  2:263943/1‑140 (MQ=255)

                               tGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCttt                                                                                                        >  2:2036432/1‑140 (MQ=255)

                                gACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCtttt                                                                                                       <  1:1292364/140‑1 (MQ=255)

                                   ttttATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAg                                                                                                    >  2:867938/1‑140 (MQ=255)

                                     ttATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTc                                                                                                  >  1:1004823/1‑140 (MQ=255)

                                      tATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTcc                                                                                                 >  2:89326/1‑140 (MQ=255)

                                        tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAg                                                                                               >  2:900782/1‑140 (MQ=255)

                                        tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAg                                                                                               >  1:1178859/1‑140 (MQ=255)

                                        tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAg                                                                                               <  2:1256515/140‑1 (MQ=255)

                                        tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAg                                                                                               <  1:89326/140‑1 (MQ=255)

                                        tGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAg                                                                                               >  2:777933/1‑140 (MQ=255)

                                          ccGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCg                                                                                             <  2:1729460/140‑1 (MQ=255)

                                             aTAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTg                                                                                          <  2:628441/140‑1 (MQ=255)

                                             aTAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTg                                                                                          >  2:1020197/1‑140 (MQ=255)

                                                       cGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCgg                                                                                >  1:1274272/1‑140 (MQ=255)

                                                        gTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGc                                                                               <  1:676255/140‑1 (MQ=255)

                                                          aGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTTCCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAg                                                                             <  1:937542/140‑1 (MQ=255)

                                                           gAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGc                                                                            <  1:2036432/140‑1 (MQ=255)

                                                           gAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGc                                                                            <  2:1004823/140‑1 (MQ=255)

                                                            aaaTTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCt                                                                           >  1:1258009/1‑140 (MQ=255)

                                                                tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTcccc                                                                       <  2:1615217/140‑1 (MQ=255)

                                                                tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTcccc                                                                       <  2:575408/140‑1 (MQ=255)

                                                                tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTcccc                                                                       <  1:729715/140‑1 (MQ=255)

                                                                      cgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGt                                                                 <  2:575260/140‑1 (MQ=255)

                                                                      cgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGt                                                                 >  1:196506/1‑140 (MQ=255)

                                                                        tCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTa                                                               >  1:1020940/1‑140 (MQ=255)

                                                                              aTGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTgg                                                         <  1:768927/140‑1 (MQ=255)

                                                                              aTGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTgg                                                         <  2:1274272/140‑1 (MQ=255)

                                                                                 tgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTggggg                                                      <  2:1178859/140‑1 (MQ=255)

                                                                                    tGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGgaga                                                   <  2:952327/140‑1 (MQ=255)

                                                                                     ggAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagat                                                  >  2:1966166/1‑140 (MQ=255)

                                                                                        agagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagataga                                               <  1:1928559/140‑1 (MQ=255)

                                                                                         gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              <  2:1145905/140‑1 (MQ=255)

                                                                                         gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              <  2:1258009/140‑1 (MQ=255)

                                                                                         gagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              >  1:1150869/1‑140 (MQ=255)

                                                                                           gTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTa                                            >  2:95897/1‑140 (MQ=255)

                                                                                           gTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTa                                            >  1:1011567/1‑140 (MQ=255)

                                                                                            tACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              <  2:231135/137‑1 (MQ=255)

                                                                                            tACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              >  1:231135/1‑137 (MQ=255)

                                                                                            tACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAg                                           >  2:1899216/1‑140 (MQ=255)

                                                                                               cATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTg                                        <  1:263943/140‑1 (MQ=255)

                                                                                                 tGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagatagat                                              <  2:1511419/132‑1 (MQ=255)

                                                                                                 tGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGagataga                                               >  1:1687403/1‑131 (MQ=255)

                                                                                                 tGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTa                                      >  1:100230/1‑140 (MQ=255)

                                                                                                 tGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGAAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTa                                      >  1:1403820/1‑140 (MQ=255)

                                                                                                   cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACa                                    >  1:931381/1‑140 (MQ=255)

                                                                                                   cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACa                                    >  1:1576442/1‑140 (MQ=255)

                                                                                                   cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCACCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACa                                    >  2:1571525/1‑140 (MQ=255)

                                                                                                    tATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGGCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGt                                          <  1:1307217/133‑1 (MQ=255)

                                                                                                      tttttGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTa                                 >  1:2059547/1‑140 (MQ=255)

                                                                                                        tttGCTGAGCGGATGATTCAGCACCCGCCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTAcc                               >  2:413308/1‑140 (MQ=255)

                                                                                                           gCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACg                            >  2:771210/1‑140 (MQ=255)

                                                                                                            cTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGa                           >  2:279418/1‑140 (MQ=255)

                                                                                                               aGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGAttt                        >  2:1939700/1‑140 (MQ=255)

                                                                                                                gCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGAtttt                       >  1:339783/1‑140 (MQ=255)

                                                                                                                gCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGAtttt                       >  2:1614842/1‑140 (MQ=255)

                                                                                                                gCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGAtttt                       >  1:1647172/1‑140 (MQ=255)

                                                                                                                gCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGAtttt                       >  1:1159989/1‑140 (MQ=255)

                                                                                                                         tCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCt              <  1:1939700/140‑1 (MQ=255)

                                                                                                                         tCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCt              <  1:1698054/140‑1 (MQ=255)

                                                                                                                         tCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCt              <  2:1647172/140‑1 (MQ=255)

                                                                                                                         tCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGCGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCt              <  1:1386112/140‑1 (MQ=255)

                                                                                                                         tCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGCTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCt              >  2:132186/1‑140 (MQ=255)

                                                                                                                           aGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCa            >  1:1298723/1‑140 (MQ=255)

                                                                                                                               cccGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCattat        <  2:1159989/140‑1 (MQ=255)

                                                                                                                                ccGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCattatt       >  2:1596179/1‑140 (MQ=255)

                                                                                                                                  ggcggTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTg     >  2:818236/1‑140 (MQ=255)

                                                                                                                                  ggcggTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTg     >  2:592502/1‑140 (MQ=255)

                                                                                                                                  ggcggTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTg     >  1:1816118/1‑140 (MQ=255)

                                                                                                                                    cggTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGcc   <  1:785312/140‑1 (MQ=255)

                                                                                                                                     ggTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGccc  <  2:1150869/140‑1 (MQ=255)

CAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCACGATTTTGACTCGGCTCATTATTTGCCC  >  NC_000913/19430‑19702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`