Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	663,547	C→A	S139S (TCG→TCT)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase DacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	663,547	0	C	A	100.0%	336.8 / NA	94	S139S (TCG→TCT)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (50/44);  total (50/44)

CAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTT  >  NC_000913/663411‑663683                                                                                                                                         |                                                                                                                                         cAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGAt                                                                                                                                       >  1:605009/1‑140 (MQ=255) cAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGAt                                                                                                                                       >  2:2017714/1‑140 (MQ=255) cAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGAt                                                                                                                                       >  1:319688/1‑140 (MQ=255)   gCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     <  2:1124798/140‑1 (MQ=255)   gCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     <  1:25044/140‑1 (MQ=255)   gCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     <  2:418977/140‑1 (MQ=255)      tCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     >  1:229502/1‑137 (MQ=255)      tCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     <  2:229502/137‑1 (MQ=255)      tCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAg                                                                                                                                  >  2:1536448/1‑140 (MQ=255)        cAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGt                                                                                                                                <  2:538300/140‑1 (MQ=255)           aCCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGt                                                                                                                                <  2:1840552/137‑1 (MQ=255)           aCCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGt                                                                                                                                >  1:1840552/1‑137 (MQ=255)                gTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     <  2:1323854/127‑1 (MQ=255)                gTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTg                                                                                                                                     >  1:1323854/1‑127 (MQ=255)                   ccGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCgg                                                                                                                     <  1:2017714/140‑1 (MQ=255)                     gTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGAt                                                                                                                   >  2:1844907/1‑140 (MQ=255)                     gTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGAt                                                                                                                   >  1:1517548/1‑140 (MQ=255)                      tCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATc                                                                                                                  >  2:153166/1‑140 (MQ=255)                      tCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATc                                                                                                                  <  1:153166/140‑1 (MQ=255)                       cTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATc                                                                                                                  <  2:710218/139‑1 (MQ=255)                       cTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATc                                                                                                                  >  1:710218/1‑139 (MQ=255)                       cTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCa                                                                                                                 >  2:846967/1‑140 (MQ=255)                       cTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCa                                                                                                                 >  2:274719/1‑140 (MQ=255)                        tGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAg                                                                                                                <  2:283307/140‑1 (MQ=255)                             gTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTgag                                                                                                           <  1:340735/140‑1 (MQ=255)                             gTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTgag                                                                                                           <  1:1923765/140‑1 (MQ=255)                                ggTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGaaa                                                                                                        >  2:2056492/1‑140 (MQ=255)                                  tATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAAcc                                                                                                      >  1:1406466/1‑140 (MQ=255)                                    tttttCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCgg                                                                                                    >  2:1099273/1‑140 (MQ=255)                                     ttttCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGa                                                                                                   <  1:1099273/140‑1 (MQ=255)                                       ttCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAAc                                                                                                 >  2:84239/1‑140 (MQ=255)                                                 gTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGccc                                                                                       >  2:572306/1‑140 (MQ=255)                                                    cGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGt                                                                                    <  2:828365/140‑1 (MQ=255)                                                    cGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGt                                                                                    <  1:572306/140‑1 (MQ=255)                                                    cGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGt                                                                                    <  2:1865648/140‑1 (MQ=255)                                                      ttAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGttt                                                                                  >  2:1421064/1‑140 (MQ=255)                                                      ttAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGttt                                                                                  >  1:316872/1‑140 (MQ=255)                                                       tAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTg                                                                                 <  2:1406466/140‑1 (MQ=255)                                                          cGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAgg                                                                              <  1:1558181/140‑1 (MQ=255)                                                          cGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAgg                                                                              >  1:2043154/1‑140 (MQ=255)                                                          cGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAgg                                                                              >  1:1511648/1‑140 (MQ=255)                                                              gCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACa                                                                          >  2:459896/1‑140 (MQ=255)                                                              gCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACa                                                                          >  2:1524282/1‑140 (MQ=255)                                                              gCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACa                                                                          >  2:1422160/1‑140 (MQ=255)                                                                tGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGGACATc                                                                        <  1:1729925/140‑1 (MQ=255)                                                                tGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATc                                                                        <  2:277252/140‑1 (MQ=255)                                                                  ttCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGcc                                                                                        >  1:1078448/1‑122 (MQ=255)                                                                  ttCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGcc                                                                                        <  2:1078448/122‑1 (MQ=255)                                                                   tcatcaAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGc                                                                     >  2:926540/1‑140 (MQ=255)                                                                       caAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  1:274719/140‑1 (MQ=255)                                                                       caAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  2:1517548/140‑1 (MQ=255)                                                                       caAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  1:1815656/140‑1 (MQ=255)                                                                       caAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  1:414684/140‑1 (MQ=255)                                                                         aGCCAACAAAAGCGTCCTGGCTACCAGCGGGAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaagaa                                                               >  2:683967/1‑140 (MQ=255)                                                                         aGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATc                                                                        >  1:1997419/1‑131 (MQ=255)                                                                         aGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATc                                                                        <  2:1997419/131‑1 (MQ=255)                                                                         aGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaagaa                                                               >  2:237840/1‑140 (MQ=255)                                                                            caacaaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  2:1615161/135‑1 (MQ=255)                                                                            caacaaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 <  1:1887607/135‑1 (MQ=255)                                                                            caacaaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 >  2:1887607/1‑135 (MQ=255)                                                                            caacaaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCgaag                                                                 >  1:1615161/1‑135 (MQ=255)                                                                               caaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCg                                                                    >  2:845561/1‑129 (MQ=255)                                                                               caaAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCg                                                                    <  1:845561/129‑1 (MQ=255)                                                                                 aaaGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTaaa                                                       <  1:1524282/140‑1 (MQ=255)                                                                                    gCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAacac                                                    <  2:605009/140‑1 (MQ=255)                                                                                    gCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAacac                                                    >  1:2039157/1‑140 (MQ=255)                                                                                       tCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCgg                                                 >  1:1889452/1‑140 (MQ=255)                                                                                       tCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCgg                                                 >  1:386204/1‑140 (MQ=255)                                                                                         cTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGt                                               <  2:1927904/140‑1 (MQ=255)                                                                                         cTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGt                                               <  2:319688/140‑1 (MQ=255)                                                                                         cTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGt                                               <  2:386204/140‑1 (MQ=255)                                                                                          tGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGtt                                              >  2:506619/1‑140 (MQ=255)                                                                                            gCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTAc                                            >  1:1532294/1‑140 (MQ=255)                                                                                               aCCAGCGGGAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCgg                                         <  1:683967/140‑1 (MQ=255)                                                                                               aCCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCgg                                         >  2:1710417/1‑140 (MQ=255)                                                                                                ccAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGt                                        <  1:1690215/140‑1 (MQ=255)                                                                                                  aGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTgg                                      >  2:175660/1‑140 (MQ=255)                                                                                                      gCAAAATCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCa                                  >  2:534355/1‑140 (MQ=255)                                                                                                          aaTCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcg                              <  2:316872/140‑1 (MQ=255)                                                                                                          aaTCGGCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcg                              <  1:1844907/140‑1 (MQ=255)                                                                                                              ggCCATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcgtcgt                          >  1:515538/1‑140 (MQ=255)                                                                                                              ggCCATGGCGACACAAGCATCATTACCAGATTGCAGGTTAATACCACGGATCAGCTGAGAAACCGGAACCTGCAAGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcgtcgt                          >  1:1707653/1‑140 (MQ=255)                                                                                                                 cATGGCGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGc                       >  2:753469/1‑140 (MQ=255)                                                                                                                      cGACACAAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATa                  <  1:506619/140‑1 (MQ=255)                                                                                                                          acaAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGa              >  1:168492/1‑140 (MQ=255)                                                                                                                           caAGCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGAc             <  1:459896/140‑1 (MQ=255)                                                                                                                              gCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcgtc                            <  2:899090/122‑1 (MQ=255)                                                                                                                              gCATCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGcgtc                            >  1:899090/1‑122 (MQ=255)                                                                                                                                aTCGTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAAt        >  2:1181061/1‑140 (MQ=255)                                                                                                                                   gTTACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAg     >  1:408301/1‑140 (MQ=255)                                                                                                                                    ttACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGt    <  2:465088/140‑1 (MQ=255)                                                                                                                                    ttACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGt    >  2:1916412/1‑140 (MQ=255)                                                                                                                                    ttACCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTAAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGt    <  1:1421064/140‑1 (MQ=255)                                                                                                                                      aCCAGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGttt  >  2:725747/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGCATCCAGACCATGTACCGTCTGGAAGTGGGTATTTTTCAGGCCCAGTGCGTTAACGTAGCTGTTCATCAAGCCAACAAAAGCGTCCTGGCTACCAGCGGCAAAATCGGCCATGGCGACACAAGCATCGTTACCCGATTGCAGGTTAATACCGCGGATCAGCTGAGAAACCGGAACCTGCATGCCCGGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTT  >  NC_000913/663411‑663683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑