Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,061,296	G→T	A213E (GCA→GAA)	nac ←	DNA‑binding transcriptional dual regulator Nac

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,061,296	0	G	T	100.0%	117.8 / NA	35	A213E (GCA→GAA)	nac	DNA‑binding transcriptional dual regulator Nac
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/17);  total (18/17)

TGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTCC  >  NC_000913/2061159‑2061426                                                                                                                                          |                                                                                                                                   tGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTcc                                                                                                                                  >  2:368140/1‑140 (MQ=255) tGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTcc                                                                                                                                  >  2:340251/1‑140 (MQ=255)   gTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGt                                                                                                                                >  2:442961/1‑140 (MQ=255)         cGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCGCCAATAACTTTTTCCGTGAGTCg                                                                                                                          >  1:173616/1‑140 (MQ=255)           tGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGcc                                                                                                                        >  1:137627/1‑140 (MQ=255)                        tttACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGc                                                                                                           >  2:186860/1‑140 (MQ=255)                               cgcCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCtcatca                                                                                                    >  1:191600/1‑140 (MQ=255)                                 ccACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAac                                                                                                  >  2:83782/1‑140 (MQ=255)                                    caTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACg                                                                                               <  1:442961/140‑1 (MQ=255)                                    caTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACg                                                                                               <  1:481084/140‑1 (MQ=255)                                     aTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGa                                                                                              <  1:171937/140‑1 (MQ=255)                                               cgcgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg                                                                                    <  2:464557/140‑1 (MQ=255)                                               cgcgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg                                                                                    >  1:264842/1‑140 (MQ=255)                                                 cgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCa                                                                                  <  1:265800/140‑1 (MQ=255)                                                 cgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCa                                                                                  <  1:368140/140‑1 (MQ=255)                                                 cgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCa                                                                                  <  2:23635/140‑1 (MQ=255)                                                            ggTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACGCGAAGTCTAATAGCACTGTAATCACt                                                                       <  1:452169/140‑1 (MQ=255)                                                            ggTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACt                                                                       <  1:352204/140‑1 (MQ=255)                                                            ggTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACt                                                                       <  2:191600/140‑1 (MQ=255)                                                              tAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgg                                                                     <  1:340251/140‑1 (MQ=255)                                                                           cccATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGtt                                                        >  1:135582/1‑140 (MQ=255)                                                                           cccATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGtt                                                        >  1:255478/1‑140 (MQ=255)                                                                              aTGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCAt                                                     <  2:255478/140‑1 (MQ=255)                                                                                     tGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCa                                              <  2:53557/140‑1 (MQ=255)                                                                                      ggCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCaa                                             >  2:227181/1‑140 (MQ=255)                                                                                          aTCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGc                                         >  2:398326/1‑140 (MQ=255)                                                                                             gCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCAtt                                      <  2:186652/140‑1 (MQ=255)                                                                                                    tAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCa                               <  1:83782/140‑1 (MQ=255)                                                                                                             cAATAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGc                      >  1:253559/1‑140 (MQ=255)                                                                                                              aaTAGACTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGcc                     <  2:250099/140‑1 (MQ=255)                                                                                                                   aCTCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCggg                >  2:444955/1‑140 (MQ=255)                                                                                                                     tCAATTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCa              <  1:374739/140‑1 (MQ=255)                                                                                                                       aaTTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAAt            >  2:419769/1‑140 (MQ=255)                                                                                                                       aaTTTCACCAATAACTTTTTCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAAt            >  1:325950/1‑140 (MQ=255)                                                                                                                                 aTAACTTTTTCCGTGAGTCGCCGTAGGTAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTcc  >  1:422192/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   TGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTCC  >  NC_000913/2061159‑2061426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑