Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	703,519	G→A	A31A (GCC→GCT)	nagB ←	glucosamine‑6‑phosphate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	703,519	0	G	A	100.0%	58.5 / NA	18	A31A (GCC→GCT)	nagB	glucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

GACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCGGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCGG  >  NC_000913/703383‑703648                                                                                                                                         |                                                                                                                                  gACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCa                                                                                                                                >  2:450077/1‑140 (MQ=255)  aCCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATcagcag                                                                                                                               <  2:286653/140‑1 (MQ=255)            tCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGaa                                                                                                                     <  1:428570/140‑1 (MQ=255)                               cAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACg                                                                                                  >  2:450185/1‑140 (MQ=255)                                                aCCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCa                                                                                 >  2:336271/1‑140 (MQ=255)                                                         gCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGAc                                                                        <  1:361028/140‑1 (MQ=255)                                                         gCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGAc                                                                        <  1:450185/140‑1 (MQ=255)                                                               tGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTTCCGACCTGttt                                                                  >  2:117258/1‑139 (MQ=255)                                                               tGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTc                                                                  >  1:142643/1‑140 (MQ=255)                                                                    ttCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCgg                                                             >  1:506886/1‑140 (MQ=255)                                                                            aCGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAATGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAgg                                                     <  1:217257/140‑1 (MQ=255)                                                                            aCGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAgg                                                     <  2:506886/140‑1 (MQ=255)                                                                                 ttATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGAt                                                <  1:230260/140‑1 (MQ=255)                                                                                  tataGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGGCCTGTTCAGCGGTAGTCAGGGGGATc                                               <  1:465006/140‑1 (MQ=255)                                                                                    taGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAg                                             <  1:450077/140‑1 (MQ=255)                                                                                       gtggtCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGtct                                          >  2:40542/1‑140 (MQ=255)                                                                                          gtCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCat                                       >  2:459367/1‑140 (MQ=255)                                                                                                ggCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCa                                 <  1:383085/140‑1 (MQ=255)                                                                                                                           aCAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTg      <  2:142643/140‑1 (MQ=255)                                                                                                                               aCGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCgg  >  1:172448/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  GACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCGGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCGG  >  NC_000913/703383‑703648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑