Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,643	C→A	A34E (GCG→GAG)	nrfG →	putative formate‑dependent nitrite reductase complex subunit NrfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,643	0	C	A	100.0%	122.2 / NA	36	A34E (GCG→GAG)	nrfG	putative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/12);  total (24/12)

TGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTAC  >  NC_000913/4293506‑4293777                                                                                                                                          |                                                                                                                                       tgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCaggagg                                                                                                                                      >  1:282619/1‑140 (MQ=255) tgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCaggagg                                                                                                                                      >  1:280153/1‑140 (MQ=255)   aTGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTa                                                                                                                                    >  1:483106/1‑140 (MQ=255)   aTGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTa                                                                                                                                    >  1:312820/1‑140 (MQ=255)           gATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGa                                                                                                                            <  1:100103/140‑1 (MQ=255)                    gCGAGTGAGGGCGAAGCGATGAAATAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTAtcagcg                                                                                                                   >  1:224970/1‑140 (MQ=255)                          gAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTAtcagcgtcagcg                                                                                                             <  2:280153/140‑1 (MQ=255)                          gAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTAtcagcgtcagcg                                                                                                             <  2:132198/140‑1 (MQ=255)                                  aGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGc                                                                                                     >  2:473814/1‑140 (MQ=255)                                               cccAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGGACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTgc                                                                                        >  1:128192/1‑140 (MQ=255)                                                         cGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCaa                                                                              >  2:154818/1‑140 (MQ=255)                                                                  tGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGaa                                                                     >  2:465531/1‑140 (MQ=255)                                                                      gACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAgcgc                                                                 >  2:349646/1‑140 (MQ=255)                                                                           cacTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCtt                                                            >  1:248996/1‑140 (MQ=255)                                                                                    tatTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTg                                                   <  1:473814/140‑1 (MQ=255)                                                                                        gATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGc                                                  <  1:187157/137‑1 (MQ=255)                                                                                        gATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGc                                                  >  2:187157/1‑137 (MQ=255)                                                                                         aTGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGa                                              >  2:141716/1‑140 (MQ=255)                                                                                          tGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGAt                                             >  2:411532/1‑140 (MQ=255)                                                                                            gTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATaa                                           >  1:170381/1‑140 (MQ=255)                                                                                            gTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATaa                                           >  1:234223/1‑140 (MQ=255)                                                                                              aTTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATaaaa                                         >  1:144858/1‑140 (MQ=255)                                                                                                ttCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATc                                       >  2:105563/1‑140 (MQ=255)                                                                                                ttCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATc                                       >  1:485268/1‑140 (MQ=255)                                                                                                   tctGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCcgc                                    >  1:168450/1‑140 (MQ=255)                                                                                                       tgtCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTa                                <  2:128192/140‑1 (MQ=255)                                                                                                       tgtCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTa                                <  2:144858/140‑1 (MQ=255)                                                                                                         tCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAAt                              <  2:282619/140‑1 (MQ=255)                                                                                                           ggCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATcc                            >  2:15203/1‑140 (MQ=255)                                                                                                            gCAGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCca                           >  2:448148/1‑140 (MQ=255)                                                                                                              aGTTATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCcaca                         <  1:105563/140‑1 (MQ=255)                                                                                                                ttATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACaaa                       <  1:349646/140‑1 (MQ=255)                                                                                                                 tATCTGTTAAGCCCAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACaaaa                      >  1:234812/1‑140 (MQ=255)                                                                                                                             ccAAAATGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTg          >  1:277099/1‑140 (MQ=255)                                                                                                                               aaaaTGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTggg        >  2:510167/1‑140 (MQ=255)                                                                                                                                 aaTGGCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCg      >  2:225011/1‑140 (MQ=255)                                                                                                                                     gCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTAc  <  2:170381/140‑1 (MQ=255)                                                                                                                                     gCAGGAGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTAc  <  1:141716/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTAC  >  NC_000913/4293506‑4293777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑