Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,868,026	+C	coding (299/333 nt)	yidQ →	DUF1375 domain‑containing putative lipoprotein YidQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,868,025	1	.	C	100.0%	71.2 / NA	21	A100P (GCG→CCG)	yidQ	DUF1375 domain‑containing putative lipoprotein YidQ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (9/12);  total (9/12)

CTAAGTCGCTGGCGATTCTCGATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATG‑CGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAAT  >  NC_000913/3867888‑3868159                                                                                                                                           |                                                                                                                                       cTAAGTCGCTGGCGATTCTCGATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATG‑cc                                                                                                                                      <  1:453800/140‑2 (MQ=255)                ttCTCGATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGc                                                                                                                        >  2:390209/1‑140 (MQ=255)                   tcGATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCCCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAg                                                                                                                     <  2:436664/140‑1 (MQ=255)                     gATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGaa                                                                                                                   <  1:132398/140‑1 (MQ=255)                     gATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGaa                                                                                                                   <  2:297002/140‑1 (MQ=255)                          gCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCt                                                                                                              >  2:115134/1‑140 (MQ=255)                              tttACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATa                                                                                                          >  2:220751/1‑140 (MQ=255)                                   cGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGAt                                                                                                           >  1:403703/1‑134 (MQ=255)                                   cGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGAt                                                                                                           <  2:403703/134‑1 (MQ=255)                                       gtgATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTa                                                                                                 >  1:251112/1‑140 (MQ=255)                                                     tttACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCgg                                                                                   <  2:289670/140‑1 (MQ=255)                                                     tttACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCgg                                                                                   <  1:220751/140‑1 (MQ=255)                                                            ccGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCca                                                                            >  1:416636/1‑140 (MQ=255)                                                            ccGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCca                                                                            >  2:175956/1‑140 (MQ=255)                                                                gggATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGc                                                                        <  2:36499/140‑1 (MQ=255)                                                                                aGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTtccatcc                                                        <  2:416636/140‑1 (MQ=255)                                                                                    aGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACgc                                                    <  1:434178/140‑1 (MQ=255)                                                                                                  gcgcgTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCAt                                      <  1:115134/140‑1 (MQ=255)                                                                                                         gAAAAAAGCGAAGCCAACGCGCAAGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCga                               <  2:268818/140‑1 (MQ=255)                                                                                                                                aGCCACCAATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAg        >  1:255217/1‑140 (MQ=255)                                                                                                                                   caccaATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTg     <  2:395213/140‑1 (MQ=255)                                                                                                                                      caATGCCGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAAt  >  1:3277/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       CTAAGTCGCTGGCGATTCTCGATATGCCGTTTACCGCTGTGATGGACACGCTTTTACTGCCGTGGGATGTGTTCCGCAAAGACAGCTCGGTGCGCTCGCGCGTTGAAAAAAGCGAAGCCAACGCGCAAGCCACCAATG‑CGGTTATTCCACCGGCCAGAATGCCTGATAACTGATTTAGCGATCAGTTTCGGTGATCCACAGCTGGCCGCCTTCCATCCACGCCAGCCACTGACCATCCGGCGAGAAGACCACGGCGTCCGCAGAAGGTGAAT  >  NC_000913/3867888‑3868159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑