Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,448	C→A	A21E (GCG→GAG)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,448	0	C	A	100.0%	76.9 / NA	24	A21E (GCG→GAG)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/13);  total (11/13)

CAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGAC  >  NC_000913/70318‑70570                                                                                                                                   |                                                                                                                           cAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAAc                                                                                                                   >  1:149031/1‑140 (MQ=255)  aGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACg                                                                                                                  >  2:266106/1‑140 (MQ=255)   gTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGc                                                                                                                 <  1:349813/140‑1 (MQ=255)                    tGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATAGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGt                                                                                                >  1:3836/1‑140 (MQ=255)                                  ttGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttttt                                                                                  >  2:116705/1‑140 (MQ=255)                                       ttcttcTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcg                                                                             >  1:438375/1‑140 (MQ=255)                                         cttctCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgac                                                                           <  2:438375/140‑1 (MQ=255)                                          ttctCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc                                                                          <  1:146120/140‑1 (MQ=255)                                            ctctGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga                                                                        <  2:149031/140‑1 (MQ=255)                                               tGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGaccgaccg                                                                     <  1:109234/140‑1 (MQ=255)                                                gAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGc                                                                    >  1:262943/1‑140 (MQ=255)                                                  aTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTg                                                                  <  1:266106/140‑1 (MQ=255)                                                        ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATg                                                            <  1:445634/140‑1 (MQ=255)                                                          aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGaa                                                          <  1:18915/140‑1 (MQ=255)                                                             aTGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAgg                                                       >  2:22807/1‑140 (MQ=255)                                                                    gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATAtt                                                <  2:264713/140‑1 (MQ=255)                                                                      aTGGCTGAAGCGCATAATCATCCCCTGCTGCCGGGATAATCGTTTAACGCCGATCTGGTGGAGGGGTTAACGCCGATTGAGGCCAGCGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATtct                                              >  2:353350/1‑140 (MQ=255)                                                                       tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATtctc                                             >  2:372241/1‑140 (MQ=255)                                                                              aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCa                                      >  1:171816/1‑140 (MQ=255)                                                                                        gATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCgg                            <  1:22807/140‑1 (MQ=255)                                                                                                          tACTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCA‑GGGGTGGTGAAAAAt         <  2:436071/140‑1 (MQ=255)                                                                                                            cTCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATc        <  1:1594/140‑1 (MQ=255)                                                                                                             tCGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCa       <  2:4549/140‑1 (MQ=255)                                                                                                              cGTTTAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAg      >  1:182711/1‑140 (MQ=255)                                                                                                                  tAACGCCCATCTGGTGGAGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGAc  >  1:379253/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                           CAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGAC  >  NC_000913/70318‑70570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑