Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,696	C→A	A688A (GCC→GCA)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,696	0	C	A	100.0%	58.8 / NA	18	A688A (GCC→GCA)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/10);  total (8/10)

GCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAAC  >  NC_000913/3224578‑3224828                                                                                                                       |                                                                                                                                     gcgACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTAt                                                                                                                 >  1:217027/1‑140 (MQ=255) gcgACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTAt                                                                                                                 >  2:342278/1‑140 (MQ=255)             cGAACAGCGAAGCCCCCTTGCAGATCGAGTTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATGCCCGCCCCCGCTACAGCGAAGCAGGACACCCTATCGCCCCTTATCAGTTACCGCTg                                                                                                     <  2:334280/140‑1 (MQ=255)             cGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTg                                                                                                     <  2:140050/140‑1 (MQ=255)             cGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTg                                                                                                     <  2:263801/140‑1 (MQ=255)             cGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTg                                                                                                     <  1:134287/140‑1 (MQ=255)             cGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTg                                                                                                     <  1:80452/140‑1 (MQ=255)                            ccTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACcgcg                                                                                      <  1:42077/140‑1 (MQ=255)                                           tGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTc                                                                       <  1:431106/140‑1 (MQ=255)                                                       aCGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAaataat                                                           <  1:54629/140‑1 (MQ=255)                                                                     ttCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGAc                                             >  2:247894/1‑140 (MQ=255)                                                                                tACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACg                                  >  1:99831/1‑140 (MQ=255)                                                                                tACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACg                                  <  2:390400/140‑1 (MQ=255)                                                                                  cGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGAt                                >  2:227025/1‑140 (MQ=255)                                                                                  cGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGAt                                >  1:317454/1‑140 (MQ=255)                                                                                        ccAAAGATTCCCGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTg                          >  1:262912/1‑140 (MQ=255)                                                                                                 cccGCACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCAcc                 >  2:426674/1‑140 (MQ=255)                                                                                                     cACCCGCTACAGCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTc             <  2:262912/140‑1 (MQ=255)                                                                                                                gCGAAGCAGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAAc  <  2:99831/140‑1 (MQ=255)                                                                                                                       |                                                                                                                                     GCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAAC  >  NC_000913/3224578‑3224828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑