Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,201,797	G→T	intergenic (‑109/‑129)	zraP ← / → zraS	zinc responsive, periplasmic protein with chaperone activity/sensory histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,201,797	0	G	T	100.0%	81.8 / NA	24	intergenic (‑109/‑129)	zraP/zraS	zinc responsive, periplasmic protein with chaperone activity/sensory histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/11);  total (13/11)

GGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTT  >  NC_000913/4201665‑4201932                                                                                                                                     |                                                                                                                                        ggCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCctgctg                                                                                                                                  >  1:150840/1‑140 (MQ=255)       tttCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTa                                                                                                                            <  2:417447/140‑1 (MQ=255)        ttCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAg                                                                                                                           <  2:260968/140‑1 (MQ=255)                                      gTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                             <  2:401822/140‑1 (MQ=255)                                      gTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                             <  1:331528/140‑1 (MQ=255)                                      gTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGc                                                                                             <  2:191343/140‑1 (MQ=255)                                                 aCTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTAc                                                                                  >  2:295317/1‑140 (MQ=255)                                                               aTCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgc                                                                    <  1:211644/140‑1 (MQ=255)                                                                 cTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAgcgc                                                                  <  1:478/140‑1 (MQ=255)                                                                            aCGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGAt                                                       >  2:125226/1‑140 (MQ=255)                                                                                                cGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAg                                   >  1:148542/1‑140 (MQ=255)                                                                                                 gggTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGa                                  <  1:136370/140‑1 (MQ=255)                                                                                                  ggTAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTTTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGaa                                 >  1:196149/1‑140 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTGGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAg                               >  1:442658/1‑140 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctg                                          <  1:400584/129‑1 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTctg                                          >  2:400584/1‑129 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAg                               >  1:215845/1‑140 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAg                               >  2:35245/1‑140 (MQ=255)                                                                                                    tAATCTGGCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAg                               >  2:42744/1‑140 (MQ=255)                                                                                                           gCATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTGGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAg                        <  2:442658/140‑1 (MQ=255)                                                                                                            cATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGc                       >  1:343252/1‑140 (MQ=255)                                                                                                            cATGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGc                       >  2:471625/1‑140 (MQ=255)                                                                                                             aTGATAAACGAGTAAAAATGACTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCa                      <  1:173832/140‑1 (MQ=255)                                                                                                                                 aCTCTCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGtt  >  2:206148/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        GGCAATTTTCGTGTTCCGTTTCATGGTTAATCCTCCAGTGGTTGTCTTACTTCGGGTATTGCATCTTCCGTGCCAACGATGAAACGCTGATATGACGGGTAATCTGGCATGATAAACGAGTAAAAATGACTCGCCTGCTGCGGGTAGCGAGTCATTTTTACTCATTGAAACTTAAGCCTTTGTGTTACAGCGCAGGGTAAGCGCTGATAAAAGATGGCATGATTTCTGCTGTCAGAAAGGGATGAGCAGGCAAAGAAGAAGATGCGTT  >  NC_000913/4201665‑4201932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑