Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,248,334	G→T	G122C (GGC→TGC)	lamB →	maltose outer membrane channel/phage lambda receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,248,334	0	G	T	100.0%	109.4 / NA	32	G122C (GGC→TGC)	lamB	maltose outer membrane channel/phage lambda receptor protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/15);  total (17/15)

AAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGTAAACTCTCT  >  NC_000913/4248200‑4248465                                                                                                                                       |                                                                                                                                    aaGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTcc                                                                                                                                <  2:49767/140‑1 (MQ=255) aaGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTcc                                                                                                                                >  2:318842/1‑140 (MQ=255)     gggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTccacca                                                                                                                            >  1:340076/1‑140 (MQ=255)     gggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTccacc                                                                                                                             >  2:341927/1‑139 (MQ=255)     gggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTccacc                                                                                                                             <  1:341927/139‑1 (MQ=255)      ggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCAt                                                                                                                           >  1:205421/1‑140 (MQ=255)      ggCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCAt                                                                                                                           <  1:227365/140‑1 (MQ=255)                  ttCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTaa                                                                                                               <  2:258133/140‑1 (MQ=255)                    cTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAgc                                                                                                             >  1:344972/1‑140 (MQ=255)                           gACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTa                                                                                                      >  1:252927/1‑140 (MQ=255)                             cacTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTAcc                                                                                                    <  2:340076/140‑1 (MQ=255)                                        ccTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGAc                                                                                         <  1:111613/140‑1 (MQ=255)                                            ttCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTc                                                                                     >  2:425239/1‑140 (MQ=255)                                             tCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCa                                                                                    <  1:193856/140‑1 (MQ=255)                                                 tCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATg                                                                                >  2:401821/1‑140 (MQ=255)                                                 tCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATg                                                                                >  2:150322/1‑140 (MQ=255)                                                           gAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACtttt                                                                      <  1:318842/140‑3 (MQ=255)                                                                   gggAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTgg                                                              >  2:440247/1‑140 (MQ=255)                                                                    ggAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATc                                                                             <  1:362140/124‑1 (MQ=255)                                                                    ggAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATc                                                                             >  2:362140/1‑124 (MQ=255)                                                                     gAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGa                                                            <  2:371304/140‑1 (MQ=255)                                                                      aaGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGat                                                           <  2:205421/140‑1 (MQ=255)                                                                                       ttCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGc                                          <  1:425239/140‑1 (MQ=255)                                                                                           gTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGt                                      <  2:171871/140‑1 (MQ=255)                                                                                                         cAGGGTAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGa                        >  2:443868/1‑140 (MQ=255)                                                                                                           gggCAAAAACCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATg                      >  2:110851/1‑140 (MQ=255)                                                                                                                 aaaCCTGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCt                <  1:396126/140‑1 (MQ=255)                                                                                                                      tGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGt           >  2:261181/1‑140 (MQ=255)                                                                                                                      tGATCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGt           >  1:180706/1‑140 (MQ=255)                                                                                                                         tCGAATGGCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGTaaa        <  1:110851/140‑1 (MQ=255)                                                                                                                               ggCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGTAAActctct  >  1:183684/1‑140 (MQ=255)                                                                                                                               ggCTGCCATGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGTAAActctct  >  1:383217/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    AAGAGGGCGATAAGAGCTTCTATTTCGACACTAACGTGGCCTATTCCGTCGCACAACAGAATGACTGGGAAGCTACCGATCCGGCCTTCCGTGAAGCAAACGTGCAGGGTAAAAACCTGATCGAATGGCTGCCAGGCTCCACCATCTGGGCAGGTAAGCGCTTCTACCAACGTCATGACGTTCATATGATCGACTTCTACTACTGGGATATTTCTGGTCCTGGTGCCGGTCTGGAAAACATCGATGTTGGCTTCGGTAAACTCTCT  >  NC_000913/4248200‑4248465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑