Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,007	C→A	A341E (GCG→GAG)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,007	0	C	A	100.0%	122.8 / NA	36	A341E (GCG→GAG)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/20);  total (16/20)

TAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCG  >  NC_000913/421879‑422139                                                                                                                                 |                                                                                                                                     tAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTg                                                                                                                           <  2:2066/140‑1 (MQ=255)   aCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCg                                                                                                                         <  2:285662/140‑1 (MQ=255)    cAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCgg                                                                                                                        <  2:54082/140‑1 (MQ=255)     aGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgt                                                                                                                       <  2:8535/140‑1 (MQ=255)     aGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgt                                                                                                                       <  2:214469/140‑1 (MQ=255)       tGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgtgt                                                                                                                     >  1:169811/1‑140 (MQ=255)        gATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTa                                                                                                                    >  1:16402/1‑140 (MQ=255)          tGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGACGACTACCGAGCTGCTGTTTGCGGTGTATc                                                                                                                  >  2:217365/1‑140 (MQ=255)            tATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTg                                                                                                                <  1:382388/140‑1 (MQ=255)              tttGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGaa                                                                                                              >  2:196294/1‑140 (MQ=255)                 ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTTGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTa                                                                                                           >  2:105846/1‑140 (MQ=255)                 ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCg                                                                                                                         <  1:190906/126‑1 (MQ=255)                 ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGACGACTACCGAGCTGCTGTTTGCg                                                                                                                         >  2:190906/1‑126 (MQ=255)                        gCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATg                                                                                                    >  2:12083/1‑140 (MQ=255)                        gCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATg                                                                                                    >  2:163426/1‑140 (MQ=255)                            tATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCgg                                                                                                >  2:251434/1‑140 (MQ=255)                                  ccACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACg                                                                                          <  1:12083/140‑1 (MQ=255)                                    aCGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTc                                                                                        <  2:197905/140‑1 (MQ=255)                                          tGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTg                                                                                  >  2:428082/1‑140 (MQ=255)                                                agCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGAtc                                                                            >  1:81661/1‑140 (MQ=255)                                                     ggCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGAtcgcttc                                                                       >  2:194628/1‑140 (MQ=255)                                                          cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  <  1:310764/140‑1 (MQ=255)                                                          cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  1:306433/1‑140 (MQ=255)                                                          cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  2:12743/1‑140 (MQ=255)                                                          cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  2:381283/1‑140 (MQ=255)                                                                      tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  2:285293/140‑1 (MQ=255)                                                                      tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  1:163426/140‑1 (MQ=255)                                                                      tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  2:306433/140‑1 (MQ=255)                                                                           gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtg                                                 <  1:12743/140‑1 (MQ=255)                                                                           gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtg                                                 <  2:169811/140‑1 (MQ=255)                                                                            cAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgt                                                <  2:268542/140‑1 (MQ=255)                                                                                aCGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggt                                            <  1:304578/140‑1 (MQ=255)                                                                                 cgtcgcgtcgCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggtg                                           <  2:451043/140‑1 (MQ=255)                                                                                   tcgcgtcgcgGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggtgtg                                         <  2:81661/140‑1 (MQ=255)                                                                                                    gTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGt                        <  1:105846/140‑1 (MQ=255)                                                                                                                          aCTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCg  >  1:273228/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                     TAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCG  >  NC_000913/421879‑422139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑