Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,587,684	T→G	L17F (TTA→TTC)	ydeQ ←	putative fimbrial adhesin protein YdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,587,684	0	T	G	100.0%	106.2 / NA	30	L17F (TTA→TTC)	ydeQ	putative fimbrial adhesin protein YdeQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/17);  total (13/17)

AGACAAGTCTACAACCAGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATTAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAGGTGTAGA  >  NC_000913/1587552‑1587809                                                                                                                                     |                                                                                                                              agaCAAGTCTACAACCAGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATagaa                                                                                                                        >  2:424/1‑140 (MQ=255)      aGTCTACAACCAGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATaa                                                                                                                   >  1:128650/1‑140 (MQ=255)                 aGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTCCCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAg                                                                                                        <  1:255030/140‑1 (MQ=255)                 aGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAg                                                                                                        <  1:93909/140‑1 (MQ=255)                 aGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAg                                                                                                        <  1:312220/140‑1 (MQ=255)                  gATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAAc                                                                                                          >  2:387377/1‑137 (MQ=255)                  gATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAAc                                                                                                          <  1:387377/137‑1 (MQ=255)                  gATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAgg                                                                                                       >  2:43141/1‑140 (MQ=255)                  gATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAg                                                                                                        <  1:335005/139‑1 (MQ=255)                  gATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAg                                                                                                        >  2:335005/1‑139 (MQ=255)                             ggCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAGCTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACt                                                                                            >  2:193523/1‑140 (MQ=255)                               cTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGa                                                                                          <  1:229457/140‑1 (MQ=255)                                          ggTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATCCTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTAccc                                                                               <  1:284348/140‑1 (MQ=255)                                          ggTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTAccc                                                                               <  1:200793/140‑1 (MQ=255)                                            tCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCAt                                                                             >  1:157236/1‑140 (MQ=255)                                                          aaCAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAgg                                                               >  1:45460/1‑140 (MQ=255)                                                                 gTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTaaaaa                                                        <  1:461179/140‑1 (MQ=255)                                                                           cTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTaaa                                              >  1:330928/1‑140 (MQ=255)                                                                           cTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTaaa                                              >  1:199026/1‑140 (MQ=255)                                                                              cAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAaag                                           <  2:92793/140‑1 (MQ=255)                                                                                      gACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTaa                                   >  2:320462/1‑140 (MQ=255)                                                                                                 gACATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAaatcgaat                        <  1:86617/140‑1 (MQ=255)                                                                                                  aCATTACAACTGAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAaatcgaatc                       <  2:263848/140‑1 (MQ=255)                                                                                                             gAAGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGc            >  1:476358/1‑140 (MQ=255)                                                                                                              aaGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGcc           <  1:424/140‑1 (MQ=255)                                                                                                              aaGGCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGcc           <  1:320462/140‑1 (MQ=255)                                                                                                                ggCAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAg         >  2:35598/1‑140 (MQ=255)                                                                                                                  cAAAACCTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAGgt       <  1:43141/140‑1 (MQ=255)                                                                                                                  cAAAAACTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAGgt       <  2:199026/140‑1 (MQ=255)                                                                                                                       aCTTTGCCTGCCATGAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAGGTGTAGa  <  2:470554/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                              AGACAAGTCTACAACCAGATTCTGGCCCGGCTGTATTACAGGGTCGAGATTAACATAAACAGAGGTTGTACCTGCTCCAATACTTGACCCGCCATCGACATTACAACTGAAGGCAAAAACTTTGCCTGCCATTAATAGAAGATAAATACCGAACAGGACCTTTATACTGATTGTTTTACCCATGATATATCCTAAGGTTAAAAATTGATTTAAAAAGAAGGCTAAGAAAATCGAATCGACGTTATTGCCAGGTGTAGA  >  NC_000913/1587552‑1587809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑