Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,822,699	G→T	V100V (GTG→GTT)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,822,699	0	G	T	100.0%	272.3 / NA	78	V100V (GTG→GTT)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/40);  total (38/40)

TGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGC  >  NC_000913/3822566‑3822834                                                                                                                                      |                                                                                                                                        tGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGaaa                                                                                                                                   <  2:1377973/140‑1 (MQ=255)  ggCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAAc                                                                                                                                  <  2:762117/140‑1 (MQ=255)   gCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACt                                                                                                                                 >  2:896682/1‑140 (MQ=255)        gaTGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATa                                                                                                                            >  1:1247939/1‑140 (MQ=255)                   aCTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTc                                                                                                                 >  2:854730/1‑140 (MQ=255)                     tATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCcg                                                                                                               <  1:1295748/140‑1 (MQ=255)                     tATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCcg                                                                                                               <  2:207132/140‑1 (MQ=255)                     tATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCcg                                                                                                               <  2:497964/140‑1 (MQ=255)                      aTGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCcgc                                                                                                              <  2:347241/140‑1 (MQ=255)                       tGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCcgcg                                                                                                             >  2:1228986/1‑140 (MQ=255)                        gAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGa                                                                                                            <  2:283432/140‑1 (MQ=255)                         aaaCGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGAt                                                                                                           <  2:680115/140‑1 (MQ=255)                         aaaCGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGAt                                                                                                           >  1:217253/1‑140 (MQ=255)                                 aTGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATaagaaaga                                                                                                   >  1:1189114/1‑140 (MQ=255)                                 aTGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATaagaaaga                                                                                                   >  2:779488/1‑140 (MQ=255)                                   ggcggcGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTGTTGAGTAGAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAgg                                                                                                 >  2:705339/1‑140 (MQ=255)                                     cggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGgcg                                                                                               >  2:231639/1‑140 (MQ=255)                                      ggcgCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGgcgc                                                                                              >  2:1455354/1‑140 (MQ=255)                                       gcgcTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCa                                                                                             >  1:231771/1‑140 (MQ=255)                                               gcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGaaa                                                                                     <  2:959232/140‑1 (MQ=255)                                                     cGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTc                                                                               <  2:848865/140‑1 (MQ=255)                                                     cGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTc                                                                               >  1:1306787/1‑140 (MQ=255)                                                     cGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTc                                                                               >  1:965913/1‑140 (MQ=255)                                                     cGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTc                                                                               >  2:1420888/1‑140 (MQ=255)                                                      gTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCg                                                                              <  1:1459871/140‑1 (MQ=255)                                                      gTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCg                                                                              <  1:896682/140‑1 (MQ=255)                                                      gTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCg                                                                              <  2:217131/140‑1 (MQ=255)                                                       tGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGc                                                                             <  1:682193/140‑1 (MQ=255)                                                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCaa                                                                           <  2:833418/140‑1 (MQ=255)                                                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCaa                                                                           <  2:608964/140‑1 (MQ=255)                                                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCaa                                                                           <  1:776298/140‑1 (MQ=255)                                                         aTTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCATCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGACGTGAAGGCCGAAAACTTTCGCaa                                                                           <  1:1228386/140‑1 (MQ=255)                                                               gATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgatgat                                                                     >  1:1287927/1‑140 (MQ=255)                                                                 tACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTa                                                                   >  1:16845/1‑140 (MQ=255)                                                                   cTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATg                                                                 >  1:1402145/1‑140 (MQ=255)                                                                   cTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATg                                                                 >  2:1228386/1‑140 (MQ=255)                                                                    tCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATgg                                                                <  1:1004029/140‑1 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             <  2:1189114/140‑1 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             >  2:1016904/1‑140 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             >  2:1259694/1‑140 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             <  1:1420888/140‑1 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             <  2:787629/140‑1 (MQ=255)                                                                       cGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             <  2:591594/140‑1 (MQ=255)                                                                         ccACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCg                                                              >  2:1114587/1‑137 (MQ=255)                                                                         ccACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCg                                                              <  2:899929/137‑1 (MQ=255)                                                                         ccACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCg                                                              <  1:1114587/137‑1 (MQ=255)                                                                         ccACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCg                                                              >  1:899929/1‑137 (MQ=255)                                                                                     atatGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATc                                               >  2:780229/1‑140 (MQ=255)                                                                                      tatGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATcc                                              >  1:1309561/1‑140 (MQ=255)                                                                                        tGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCcgc                                            >  1:1227028/1‑140 (MQ=255)                                                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             >  1:565704/1‑122 (MQ=255)                                                                                         ggAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGa                                                             <  2:565704/122‑1 (MQ=255)                                                                                          gAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGt                                          >  2:109895/1‑140 (MQ=255)                                                                                            aCAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCa                                        <  2:524814/140‑1 (MQ=255)                                                                                             cAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCAt                                       <  2:231771/140‑1 (MQ=255)                                                                                              aGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAATCTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATc                                      <  2:736935/140‑1 (MQ=255)                                                                                              aGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATc                                      >  1:66454/1‑140 (MQ=255)                                                                                              aGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATc                                      >  1:195277/1‑140 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCAt                                       <  2:1016794/137‑1 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCAt                                       >  1:1016794/1‑137 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCt                                    >  1:1473620/1‑140 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCt                                    >  2:89978/1‑140 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCt                                    >  2:1066815/1‑140 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCt                                    >  2:743266/1‑140 (MQ=255)                                                                                                cTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTAATCCt                                    >  2:1478392/1‑140 (MQ=255)                                                                                                   tttGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCtcat                                 >  2:887241/1‑140 (MQ=255)                                                                                                               gTCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGa                     <  2:551341/140‑1 (MQ=255)                                                                                                                tCGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAc                    >  1:374825/1‑140 (MQ=255)                                                                                                                 cGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAcc                   <  1:1455354/140‑1 (MQ=255)                                                                                                                 cGCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAcc                   <  1:887241/140‑1 (MQ=255)                                                                                                                  gCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCg                  <  2:1332580/140‑1 (MQ=255)                                                                                                                  gCCGAGCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCg                  <  1:658765/140‑1 (MQ=255)                                                                                                                       gCTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAccc             >  2:144428/1‑140 (MQ=255)                                                                                                                        cTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAc                    >  2:1058919/1‑132 (MQ=255)                                                                                                                        cTGGTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGAc                    <  1:1058919/132‑1 (MQ=255)                                                                                                                           gTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCacaa         <  1:1066815/140‑1 (MQ=255)                                                                                                                           gTAGAGGGGGTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCacaa         <  2:620161/140‑1 (MQ=255)                                                                                                                                 ggggTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATgcg   <  2:1309561/140‑1 (MQ=255)                                                                                                                                 ggggTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATgcg   <  2:1247939/140‑1 (MQ=255)                                                                                                                                  gggTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATgcgc  >  2:922569/1‑140 (MQ=255)                                                                                                                                  gggTTTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATgcac  <  2:1473620/140‑3 (MQ=255)                                                                                                                                      |                                                                                                                                        TGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGC  >  NC_000913/3822566‑3822834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑