Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,006,045	C→A	A61D (GCC→GAC)	recQ →	ATP‑dependent DNA helicase RecQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,006,045	0	C	A	100.0%	255.6 / NA	72	A61D (GCC→GAC)	recQ	ATP‑dependent DNA helicase RecQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/39);  total (33/39)

GGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTT  >  NC_000913/4005908‑4006178                                                                                                                                          |                                                                                                                                      ggTTTTACAAGAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACt                                                                                                                                     <  2:515371/140‑1 (MQ=255)    tttACAAGAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTAt                                                                                                                                  >  2:741556/1‑140 (MQ=255)           gAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTaaa                                                                                                                           <  2:698006/140‑1 (MQ=255)           gAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTaaa                                                                                                                           <  1:199159/140‑1 (MQ=255)             aaCCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACg                                                                                                                         >  2:424270/1‑140 (MQ=255)               ccTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGc                                                                                                                       <  2:989435/140‑1 (MQ=255)                 tttGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCt                                                                                                                     <  1:1007538/140‑1 (MQ=255)                 tttGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCt                                                                                                                     <  2:1078017/140‑1 (MQ=255)                    ggCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTAc                                                                                                                  <  2:1280604/140‑1 (MQ=255)                     gCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTAcc                                                                                                                 <  1:1100935/140‑1 (MQ=255)                     gCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTAcc                                                                                                                 <  1:68830/140‑1 (MQ=255)                       tACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGt                                                                                                               <  2:900756/140‑1 (MQ=255)                         ccAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTgg                                                                                                             <  1:566187/140‑1 (MQ=255)                          cAACAGGTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGgt                                                                                                            >  1:334207/1‑140 (MQ=255)                                   cGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCAcc                                                                                                   >  2:1445536/1‑140 (MQ=255)                                   cGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCAcc                                                                                                   >  2:410538/1‑140 (MQ=255)                                   cGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCAcc                                                                                                   >  1:960791/1‑140 (MQ=255)                                   cGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCAcc                                                                                                   >  1:740180/1‑140 (MQ=255)                                   cGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCAcc                                                                                                   >  2:651431/1‑140 (MQ=255)                                    gCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCg                                                                                                  <  1:181856/140‑1 (MQ=255)                                         ggCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGAt                                                                                             <  1:1066402/140‑1 (MQ=255)                                          gCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGAtt                                                                                            >  2:526636/1‑140 (MQ=255)                                             aGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCg                                                                                         <  1:60602/140‑1 (MQ=255)                                             aGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCg                                                                                         <  1:651431/140‑1 (MQ=255)                                              ggAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGt                                                                                        <  1:91221/140‑1 (MQ=255)                                               gaagaaATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGtt                                                                                       <  2:358439/140‑1 (MQ=255)                                                   aaaTTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgatg                                                                                   <  2:650775/140‑1 (MQ=255)                                                   aaaTTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgatg                                                                                   <  1:741556/140‑1 (MQ=255)                                                    aaTTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgatga                                                                                  >  2:1191815/1‑140 (MQ=255)                                                    aaTTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgatga                                                                                  <  2:1106730/140‑1 (MQ=255)                                                        aTCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGa                                                                              <  1:925523/140‑1 (MQ=255)                                                         tcaacacTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             >  2:1379355/4‑140 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgat                                                                                    <  1:33181/133‑1 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTtgat                                                                                    >  2:33181/1‑133 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             >  1:36618/1‑140 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             <  2:36618/140‑1 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             <  2:1078849/140‑1 (MQ=255)                                                         tCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             >  1:1358590/1‑140 (MQ=255)                                                           gACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCa                                                                           <  1:410538/140‑1 (MQ=255)                                                           gACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCa                                                                           <  2:1172402/140‑1 (MQ=255)                                                             cacTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAgg                                                                         >  2:547859/1‑140 (MQ=255)                                                             cacTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAgg                                                                         <  2:105552/140‑1 (MQ=255)                                                              acTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGt                                                                        <  1:804390/140‑1 (MQ=255)                                                              acTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGt                                                                        >  1:749257/1‑140 (MQ=255)                                                                tgtgCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTgg                                                                      >  1:655513/1‑140 (MQ=255)                                                                  tgCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             >  2:1387383/1‑131 (MQ=255)                                                                  tgCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             <  1:1387383/131‑1 (MQ=255)                                                                  tgCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGAt                                                                    >  1:443162/1‑140 (MQ=255)                                                                  tgCTTTCCGGCCGCGATTGCCACGTCGTCATCCCCACTGGAGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGAt                                                                    >  2:1002087/1‑140 (MQ=255)                                                                   gCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATc                                                                   >  2:519072/1‑140 (MQ=255)                                                                       tCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACt                                                               >  1:1217149/1‑140 (MQ=255)                                                                       tCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACt                                                               >  1:225086/1‑140 (MQ=255)                                                                          ggCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             >  2:325531/1‑123 (MQ=255)                                                                          ggCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAt                                                                             <  1:325531/123‑1 (MQ=255)                                                                          ggCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCa                                                            >  2:376776/1‑140 (MQ=255)                                                                            ccGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAg                                                          >  1:820138/1‑140 (MQ=255)                                                                                          tcgtcATGCCCTCTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTCCCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcg                                            <  2:899162/140‑1 (MQ=255)                                                                                          tcgtcATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcg                                            >  2:1430035/1‑140 (MQ=255)                                                                                          tcgtcATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcg                                            >  2:1064697/1‑140 (MQ=255)                                                                                               aTGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcggcggc                                       <  1:1191815/140‑1 (MQ=255)                                                                                               aTGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcggcggc                                       <  1:701802/140‑1 (MQ=255)                                                                                               aTGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTggcggcggc                                       >  2:530412/1‑140 (MQ=255)                                                                                                  cccACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTg                                    >  2:1423413/1‑140 (MQ=255)                                                                                                     aCTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCt                                 >  1:168739/1‑140 (MQ=255)                                                                                                       tggtggCGGAAAATCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTa                               >  2:1449749/1‑140 (MQ=255)                                                                                                                   aTCCCTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCaaa                   <  2:225086/140‑1 (MQ=255)                                                                                                                      ccTTTGCTATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAAccc                <  1:1423413/140‑1 (MQ=255)                                                                                                                             tATCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTAGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACa         <  1:1379355/140‑1 (MQ=255)                                                                                                                              aTCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCa                     <  2:618530/127‑1 (MQ=255)                                                                                                                              aTCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCa                     >  1:618530/1‑127 (MQ=255)                                                                                                                               tCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACa         <  2:268548/138‑1 (MQ=255)                                                                                                                               tCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACa         >  1:268548/1‑138 (MQ=255)                                                                                                                               tCAAATCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGc       >  2:1218593/1‑140 (MQ=255)                                                                                                                                    tCCCTGACTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACtt  <  1:547859/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCGCCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAATCCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGATCAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTT  >  NC_000913/4005908‑4006178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑