Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	939,768	G→T	G114V (GGT→GTT)	serS →	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	939,768	0	G	T	100.0%	307.5 / NA	86	G114V (GGT→GTT)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/44);  total (42/44)

GAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGGTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTG  >  NC_000913/939632‑939902                                                                                                                                         |                                                                                                                                       gAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTaa                                                                                                                                     >  1:1258133/1‑140 (MQ=255) gAGCCTTTACGTCTAGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTaa                                                                                                                                     >  1:1161070/1‑140 (MQ=255)   gCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAg                                                                                                                                   >  2:910615/1‑140 (MQ=255)   gCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAg                                                                                                                                   >  1:159413/1‑140 (MQ=255)   gCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAg                                                                                                                                   >  2:1075401/1‑140 (MQ=255)              tGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGAc                                                                                                                        <  1:207670/140‑1 (MQ=255)              tGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGAc                                                                                                                        >  2:276109/1‑140 (MQ=255)               ggAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAACGACa                                                                                                                       <  2:899609/140‑1 (MQ=255)                gAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACaa                                                                                                                      >  1:1400329/1‑140 (MQ=255)                  aGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACg                                                                                                                    <  1:910615/140‑1 (MQ=255)                    tGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGtt                                                                                                                  >  2:1154215/1‑140 (MQ=255)                     gAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTg                                                                                                                 >  2:371722/1‑140 (MQ=255)                     gAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTg                                                                                                                 <  1:1413669/140‑1 (MQ=255)                     gAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTg                                                                                                                 <  2:1161070/140‑1 (MQ=255)                       aCAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGaa                                                                                                               <  1:1007015/140‑1 (MQ=255)                       aCAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGaa                                                                                                               <  1:100048/140‑1 (MQ=255)                          aaCTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTc                                                                                                            <  2:1400329/140‑1 (MQ=255)                          aaCTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTc                                                                                                            >  2:650300/1‑140 (MQ=255)                           aCTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCa                                                                                                           >  1:954943/1‑140 (MQ=255)                            cTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAg                                                                                                          >  1:346378/1‑140 (MQ=255)                             tGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGc                                                                                                         <  2:1256391/140‑1 (MQ=255)                             tGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGc                                                                                                         <  1:1052223/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGTCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  1:436137/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCGGCCg                                                                                                       <  1:845678/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  2:346378/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  2:117551/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       >  1:1193319/1‑140 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  1:446934/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       >  2:102185/1‑140 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  1:99123/140‑1 (MQ=255)                               ggCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCg                                                                                                       <  1:713794/140‑1 (MQ=255)                                     gagCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTgggg                                                                                                 <  2:1453043/140‑1 (MQ=255)                                             tGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCcgc                                                                                         >  1:798384/1‑140 (MQ=255)                                                agcaAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCTATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTg                                                                                      >  1:562435/1‑140 (MQ=255)                                                    aaaGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGtt                                                                                  <  1:1011860/140‑1 (MQ=255)                                                     aaGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGttt                                                                                 <  1:303081/140‑1 (MQ=255)                                                       gCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGa                                                                               <  1:350385/140‑1 (MQ=255)                                                       gCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGa                                                                               >  2:1387502/1‑140 (MQ=255)                                                       gCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAAATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGa                                                                               >  1:637190/1‑140 (MQ=255)                                                          gAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACtt                                                                            <  1:1075401/140‑1 (MQ=255)                                                          gAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACtt                                                                            >  1:1025460/1‑140 (MQ=255)                                                            gCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTg                                                                          >  1:786838/1‑140 (MQ=255)                                                            gCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTg                                                                          >  1:454812/1‑140 (MQ=255)                                                                     tttACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTg                                                                 <  2:475911/140‑1 (MQ=255)                                                                     tttACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTg                                                                 >  1:706026/1‑140 (MQ=255)                                                                     tttACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTg                                                                 >  2:1201553/1‑140 (MQ=255)                                                                       tACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGAc                                                               <  1:1185271/140‑1 (MQ=255)                                                                            gCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGt                                                          >  2:1258270/1‑140 (MQ=255)                                                                              tGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGa                                                        >  2:985787/1‑140 (MQ=255)                                                                              tGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGa                                                        >  2:627160/1‑140 (MQ=255)                                                                              tGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGa                                                        >  2:693653/1‑140 (MQ=255)                                                                              tGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGa                                                        >  1:446749/1‑140 (MQ=255)                                                                               gAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGAc                                                       >  2:48390/1‑140 (MQ=255)                                                                               gAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGAc                                                       >  2:105414/1‑140 (MQ=255)                                                                                aaaTTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACg                                                      <  1:1258270/140‑1 (MQ=255)                                                                                 aaatCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGc                                                     <  2:1386257/137‑1 (MQ=255)                                                                                    tCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTgg                                                  <  1:1044220/140‑1 (MQ=255)                                                                                    tCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTgg                                                  <  1:820890/140‑1 (MQ=255)                                                                                     cgcgATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTggg                                                 >  1:580869/1‑140 (MQ=255)                                                                                      gcgATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGa                                                                >  2:1390554/1‑124 (MQ=255)                                                                                      gcgATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGa                                                                <  1:1390554/124‑1 (MQ=255)                                                                                      gcgATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGt                                                <  2:947969/140‑1 (MQ=255)                                                                                      gcgATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGt                                                <  1:878354/140‑1 (MQ=255)                                                                                                gcTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCAct                                      <  1:371722/140‑1 (MQ=255)                                                                                                gcTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCAct                                      <  2:897716/140‑1 (MQ=255)                                                                                                gcTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCAct                                      <  2:711389/140‑1 (MQ=255)                                                                                                    aCCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTgg                                  >  2:1069226/1‑140 (MQ=255)                                                                                                     ccATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGc                                 <  2:562435/140‑1 (MQ=255)                                                                                                        tCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTc                              <  1:1154215/140‑1 (MQ=255)                                                                                                               cctgcctgCAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTg                       <  1:370934/140‑1 (MQ=255)                                                                                                                  gcctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAg                    >  1:630165/1‑140 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   <  2:585225/140‑1 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   >  1:10848/1‑140 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   <  2:798384/140‑1 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   >  2:288364/1‑140 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   >  2:238267/1‑140 (MQ=255)                                                                                                                   cctgcAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGc                   >  1:1359867/1‑140 (MQ=255)                                                                                                                       cAGATGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCa               <  2:706026/140‑1 (MQ=255)                                                                                                                          aTGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGtt            <  1:655266/140‑1 (MQ=255)                                                                                                                          aTGAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGtt            >  1:41497/1‑140 (MQ=255)                                                                                                                            gAAGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTaa          >  2:110316/1‑140 (MQ=255)                                                                                                                             aaGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAg         <  1:276109/140‑1 (MQ=255)                                                                                                                             aaGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAg         <  1:693653/140‑1 (MQ=255)                                                                                                                              aGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGc        <  2:630165/140‑1 (MQ=255)                                                                                                                              aGTGCCGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGc        <  1:102185/140‑1 (MQ=255)                                                                                                                                  ccGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGAc    >  2:612144/1‑140 (MQ=255)                                                                                                                                  ccGGTAGTTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGAc    >  1:370926/1‑140 (MQ=255)                                                                                                                                    ggtagttaAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTg  >  1:1375141/1‑140 (MQ=255)                                                                                                                                    ggtagttaAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTg  >  2:958605/1‑140 (MQ=255)                                                                                                                                    ggtagttaAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTg  >  2:97851/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GAGCCTTTACGTCTGGAAGTGAACAAACTGGGCGAAGAGCTGGATGCAGCAAAAGCCGAGCTGGATGCTTTACAGGCTGAAATTCGCGATATCGCGCTGACCATCCCTAACCTGCCTGCAGATGAAGTGCCGGTAGGTAAAGACGAAAATGACAACGTTGAAGTCAGCCGCTGGGGTACCCCGCGTGAGTTTGACTTTGAAGTTCGTGACCACGTGACGCTGGGTGAAATGCACTCTGGCCTCGACTTTGCAGCTGCAGTTAAGCTGACTG  >  NC_000913/939632‑939902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑