Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	52,410	C→A	Q7H (CAG→CAT)	rsmA ←	16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	52,410	0	C	A	100.0%	239.0 / NA	70	Q7H (CAG→CAT)	rsmA	16S rRNA m(6)2A1518,m(6)2A1519 dimethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/41);  total (29/41)

CAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGC  >  NC_000913/52278‑52542                                                                                                                                     |                                                                                                                                     cAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGa                                                                                                                               <  2:1189784/140‑1 (MQ=255)  aaTGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGAc                                                                                                                              <  1:944867/140‑1 (MQ=255)   aTGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACt                                                                                                                             <  1:1364913/140‑1 (MQ=255)    tGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTc                                                                                                                            >  1:607490/1‑140 (MQ=255)    tGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTc                                                                                                                            >  1:713801/1‑140 (MQ=255)    tGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTc                                                                                                                            >  2:421848/1‑140 (MQ=255)       cgccAGACTGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGat                                                                                                                         <  1:1431547/140‑1 (MQ=255)       cgccAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGat                                                                                                                         <  1:663575/140‑1 (MQ=255)       cgccAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGat                                                                                                                         <  1:375134/140‑1 (MQ=255)         ccAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGatta                                                                                                                       <  2:494897/140‑1 (MQ=255)              ccGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCAt                                                                                                                  <  1:1057266/140‑1 (MQ=255)              ccGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCAt                                                                                                                  <  2:1069681/140‑1 (MQ=255)              ccGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCAt                                                                                                                  <  1:1300049/140‑1 (MQ=255)                   gCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGgt                                                                                                             <  1:765976/140‑1 (MQ=255)                            gACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGGTCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAAt                                                                                                    >  2:1482754/1‑140 (MQ=255)                                   gCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTg                                                                                             <  1:780471/140‑1 (MQ=255)                                       ggCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATgg                                                                                         <  2:607490/140‑1 (MQ=255)                                       ggCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATgg                                                                                         <  2:713801/140‑1 (MQ=255)                                        gCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGc                                                                                        >  2:1379611/1‑140 (MQ=255)                                         cccTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCg                                                                                       <  1:1067264/140‑1 (MQ=255)                                                 cGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAg                                                                               <  2:1065086/140‑1 (MQ=255)                                                    gTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAgcgc                                                                            <  2:919711/140‑1 (MQ=255)                                                       aaTGGCAGACACAATACTGTCGGTCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGt                                                                         <  1:1482754/140‑1 (MQ=255)                                                        aTGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGta                                                                        >  1:411625/1‑140 (MQ=255)                                                              gACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATaaaa                                                                  >  2:1251417/1‑140 (MQ=255)                                                                  caATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGc                                                              >  1:790619/1‑140 (MQ=255)                                                                   aaTACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGcc                                                             <  1:635789/140‑1 (MQ=255)                                                                     tACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGa                                                           >  1:309784/1‑140 (MQ=255)                                                                        tGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACAt                                                        >  1:1495287/1‑140 (MQ=255)                                                                         gTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATc                                                       >  1:52808/1‑140 (MQ=255)                                                                          tCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCg                                                      >  2:914201/1‑140 (MQ=255)                                                                          tCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCg                                                      >  2:1128908/1‑140 (MQ=255)                                                                          tCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCg                                                      >  1:312731/1‑140 (MQ=255)                                                                             aTCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCt                                                   >  1:523153/1‑140 (MQ=255)                                                                               cACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCttt                                                 >  1:710354/1‑140 (MQ=255)                                                                                 cGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGc                                               <  1:1520635/140‑1 (MQ=255)                                                                                 cGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGc                                               <  1:1379611/140‑1 (MQ=255)                                                                                 cGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGc                                               <  2:312731/140‑1 (MQ=255)                                                                                 cGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGc                                               >  2:1520635/1‑140 (MQ=255)                                                                                     cTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACg                                           <  1:723472/140‑1 (MQ=255)                                                                                        aTCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTcc                                        >  1:854641/1‑140 (MQ=255)                                                                                        aTCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTcc                                        >  2:796565/1‑140 (MQ=255)                                                                                        aTCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTcc                                        >  2:1206414/1‑140 (MQ=255)                                                                                        aTCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTcc                                        >  1:624628/1‑140 (MQ=255)                                                                                               agaAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGt                                 >  2:1156848/1‑140 (MQ=255)                                                                                                 aaaGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTc                               <  2:854641/140‑1 (MQ=255)                                                                                                  aaGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCa                              >  2:92572/1‑140 (MQ=255)                                                                                                   aGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCaa                             <  2:52808/140‑1 (MQ=255)                                                                                                       ttGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAgcgc                         >  1:484628/1‑140 (MQ=255)                                                                                                        tGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGcgcg                        <  2:1495287/140‑1 (MQ=255)                                                                                                        tGCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGcgcg                        <  2:790619/140‑1 (MQ=255)                                                                                                         gCCCGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCgg                       <  1:1128908/140‑1 (MQ=255)                                                                                                           ccGAAGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTg                     <  2:411625/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  1:1156848/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  2:345491/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  2:624628/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  2:710354/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  1:914201/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  1:92572/140‑1 (MQ=255)                                                                                                              aaGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  2:830734/140‑1 (MQ=255)                                                                                                               aGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGt                 >  2:479016/1‑140 (MQ=255)                                                                                                               aGCGTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGt                 >  2:613284/1‑140 (MQ=255)                                                                                                                  gTTTACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGt              <  2:523153/140‑1 (MQ=255)                                                                                                                   tttACGGGCTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTc             <  2:484628/140‑1 (MQ=255)                                                                                                                           cTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  <  2:1514489/127‑1 (MQ=255)                                                                                                                           cTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCg                  >  1:1514489/1‑127 (MQ=255)                                                                                                                           cTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGa       <  1:1069732/138‑1 (MQ=255)                                                                                                                           cTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGa       >  2:1069732/1‑138 (MQ=255)                                                                                                                           cTAAGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGAtg     >  1:1475928/1‑140 (MQ=255)                                                                                                                              aGTGGCCATGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGc  <  1:421848/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     CAATGCCGCCAGACCGGGGCCGATTTCGACCATCGCCTGGCCCTTTTGCGGGTTAATGGCAGACACAATACTGTCGATCACGAACTGATCGTTGAGAAAGTTTTGCCCGAAGCGTTTACGGGCTAAGTGGCCCTGGTGGACTCGATTATTCATTGGGTGTTAACAATCATTTTGATGGCGAGATTAAGCGCCGTAATAAAACTGCCGACATCGGCTTTGCCACGTCCCGCCAGTTCAAGCGCGGTGCCGTGGTCCACTGATGTGC  >  NC_000913/52278‑52542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑